Vibrio phage PVA1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Podoviridae; unclassified Podoviridae

Average proteome isoelectric point is 5.85

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 21 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|W8EIS3|W8EIS3_9CAUD Endonuclease domain protein OS=Vibrio phage PVA1 OX=1461743 GN=PVA1_42 PE=4 SV=1
MM1 pKa = 7.78SDD3 pKa = 3.22TFRR6 pKa = 11.84KK7 pKa = 9.4VVNFPVGIKK16 pKa = 8.74KK17 pKa = 8.08TAVASAAADD26 pKa = 3.52VVNLGEE32 pKa = 4.2VQALVAGLNLAKK44 pKa = 10.31EE45 pKa = 4.27AVAVDD50 pKa = 3.18THH52 pKa = 7.3DD53 pKa = 5.19NIDD56 pKa = 3.78LATGGLIVIDD66 pKa = 5.17GYY68 pKa = 8.01QTIDD72 pKa = 3.36GDD74 pKa = 3.74RR75 pKa = 11.84VLVRR79 pKa = 11.84EE80 pKa = 4.28QTDD83 pKa = 3.2SSQNGIYY90 pKa = 9.77IASSGAWSRR99 pKa = 11.84ATDD102 pKa = 3.46ADD104 pKa = 3.65EE105 pKa = 4.72AAEE108 pKa = 4.27LKK110 pKa = 10.77AKK112 pKa = 10.21TSVTVLNGPHH122 pKa = 6.69SGRR125 pKa = 11.84KK126 pKa = 8.94YY127 pKa = 10.4EE128 pKa = 4.03LQEE131 pKa = 4.17DD132 pKa = 4.27QPVVGTDD139 pKa = 2.99AQTWIVTSASSSAAVDD155 pKa = 3.59TTVDD159 pKa = 3.14TTNFDD164 pKa = 4.42RR165 pKa = 11.84INPASSNAQSALDD178 pKa = 3.86SVDD181 pKa = 4.27DD182 pKa = 4.4LLVTITDD189 pKa = 3.83TVDD192 pKa = 3.62SINGAGAGAQDD203 pKa = 3.89YY204 pKa = 7.77GTFTGSTLSDD214 pKa = 3.38NASGKK219 pKa = 9.85ILFQEE224 pKa = 4.65LEE226 pKa = 4.23TAHH229 pKa = 6.09EE230 pKa = 4.07QLVTDD235 pKa = 4.57LANDD239 pKa = 3.66RR240 pKa = 11.84FEE242 pKa = 4.56STVTTLTTGSTITFTHH258 pKa = 6.9NIGTQFLSGLKK269 pKa = 9.85VYY271 pKa = 10.91NVVEE275 pKa = 4.3GANEE279 pKa = 5.05DD280 pKa = 3.3ITHH283 pKa = 6.6TVTIVAVDD291 pKa = 3.77GNNVTVQNDD300 pKa = 3.79GDD302 pKa = 4.06PVDD305 pKa = 4.04VVVVCAKK312 pKa = 10.57

Molecular weight: 32.59 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|W8EIS8|W8EIS8_9CAUD Putative lysozyme family protein OS=Vibrio phage PVA1 OX=1461743 GN=PVA1_60 PE=4 SV=1
MM1 pKa = 6.75QQAADD6 pKa = 4.54EE7 pKa = 4.67IGLTYY12 pKa = 10.02NQVMYY17 pKa = 10.46IAGKK21 pKa = 9.29FKK23 pKa = 10.58ISFSHH28 pKa = 6.23KK29 pKa = 9.86GSRR32 pKa = 11.84HH33 pKa = 4.94YY34 pKa = 7.77MTKK37 pKa = 10.36LAEE40 pKa = 4.31CDD42 pKa = 3.08IPLIRR47 pKa = 11.84QLYY50 pKa = 9.18HH51 pKa = 6.89EE52 pKa = 4.91GTKK55 pKa = 10.28KK56 pKa = 10.23HH57 pKa = 5.62IIARR61 pKa = 11.84KK62 pKa = 8.68FDD64 pKa = 3.63VNVPVIEE71 pKa = 5.27KK72 pKa = 10.15ILSGANWSHH81 pKa = 5.62VKK83 pKa = 10.37

Molecular weight: 9.54 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

21

0

21

6223

72

694

296.3

33.05

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.645 ± 0.699

1.012 ± 0.191

6.942 ± 0.317

7.183 ± 0.486

3.375 ± 0.262

6.926 ± 0.465

1.398 ± 0.212

5.415 ± 0.197

7.087 ± 0.52

7.504 ± 0.246

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.989 ± 0.357

5.431 ± 0.354

3.567 ± 0.272

4.644 ± 0.373

4.516 ± 0.29

6.749 ± 0.293

5.769 ± 0.467

5.93 ± 0.493

1.269 ± 0.173

3.648 ± 0.411
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski