Wheat dwarf virus

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Repensiviricetes; Geplafuvirales; Geminiviridae; Mastrevirus

Average proteome isoelectric point is 7.13

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|B9W0A3|B9W0A3_9GEMI Replication-associated protein OS=Wheat dwarf virus OX=10834 GN=C1-1 PE=3 SV=1
MM1 pKa = 7.66ASSSAPRR8 pKa = 11.84FRR10 pKa = 11.84VYY12 pKa = 10.78SKK14 pKa = 11.3YY15 pKa = 11.03LFLTYY20 pKa = 8.66PQCILEE26 pKa = 4.18PQFALEE32 pKa = 4.11SLRR35 pKa = 11.84TLLAKK40 pKa = 10.31YY41 pKa = 9.47EE42 pKa = 4.16PLYY45 pKa = 10.3IAAVRR50 pKa = 11.84EE51 pKa = 4.02LHH53 pKa = 6.85EE54 pKa = 5.61DD55 pKa = 4.13GSPHH59 pKa = 6.69LHH61 pKa = 5.96VLVQNKK67 pKa = 8.63LRR69 pKa = 11.84ASITNPHH76 pKa = 6.6ALNLRR81 pKa = 11.84MDD83 pKa = 4.12TSPFNIFHH91 pKa = 7.15PNIQAAKK98 pKa = 9.81DD99 pKa = 3.72CNQVRR104 pKa = 11.84DD105 pKa = 4.27YY106 pKa = 9.75ITKK109 pKa = 10.41EE110 pKa = 3.51VDD112 pKa = 3.0SDD114 pKa = 4.11VNTAEE119 pKa = 3.82WGTFIAVTTPGRR131 pKa = 11.84KK132 pKa = 9.53DD133 pKa = 2.9RR134 pKa = 11.84DD135 pKa = 3.22ADD137 pKa = 3.4MKK139 pKa = 10.91QIIEE143 pKa = 4.28SSSSRR148 pKa = 11.84EE149 pKa = 3.73EE150 pKa = 3.92FLSMVCHH157 pKa = 6.68RR158 pKa = 11.84FPFEE162 pKa = 3.34WSIRR166 pKa = 11.84LKK168 pKa = 10.83DD169 pKa = 3.77FEE171 pKa = 4.34YY172 pKa = 10.01TARR175 pKa = 11.84HH176 pKa = 6.38LFPDD180 pKa = 4.45PVATYY185 pKa = 9.27TPEE188 pKa = 3.93FPIEE192 pKa = 3.96SLMCHH197 pKa = 5.25EE198 pKa = 5.53TIEE201 pKa = 4.32SWKK204 pKa = 10.62NEE206 pKa = 3.76HH207 pKa = 6.69LYY209 pKa = 10.78SVSLEE214 pKa = 4.09SYY216 pKa = 9.72ILCTSTPADD225 pKa = 3.56KK226 pKa = 10.8AVSDD230 pKa = 4.57LEE232 pKa = 4.2WMDD235 pKa = 4.1DD236 pKa = 3.58YY237 pKa = 11.81SRR239 pKa = 11.84SHH241 pKa = 7.22RR242 pKa = 11.84DD243 pKa = 3.15GISPYY248 pKa = 10.27TSADD252 pKa = 3.27QQEE255 pKa = 4.38QEE257 pKa = 4.27RR258 pKa = 11.84LPGQGLL264 pKa = 3.44

Molecular weight: 30.45 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|B9W0A2|B9W0A2_9GEMI Replication-associated protein OS=Wheat dwarf virus OX=10834 GN=C1-2 PE=3 SV=1
MM1 pKa = 6.79VTNKK5 pKa = 10.32DD6 pKa = 3.24SRR8 pKa = 11.84GKK10 pKa = 10.54GKK12 pKa = 10.56RR13 pKa = 11.84KK14 pKa = 9.72LDD16 pKa = 3.67EE17 pKa = 5.03ADD19 pKa = 3.75SSGRR23 pKa = 11.84WKK25 pKa = 10.54GAVYY29 pKa = 10.24KK30 pKa = 10.38RR31 pKa = 11.84RR32 pKa = 11.84KK33 pKa = 7.79QAYY36 pKa = 8.07KK37 pKa = 10.14VVPVKK42 pKa = 10.61PPALCVYY49 pKa = 9.96RR50 pKa = 11.84YY51 pKa = 9.78NWLNSDD57 pKa = 3.29KK58 pKa = 11.2TNIVVGNTPRR68 pKa = 11.84VDD70 pKa = 5.69LITCFAAGKK79 pKa = 10.15ADD81 pKa = 3.89NNRR84 pKa = 11.84HH85 pKa = 4.81TNQTVLYY92 pKa = 8.93KK93 pKa = 10.21FNIQGTCYY101 pKa = 10.02VADD104 pKa = 3.51VSSIYY109 pKa = 10.38VGPIRR114 pKa = 11.84VYY116 pKa = 9.85HH117 pKa = 5.66WLVYY121 pKa = 10.26DD122 pKa = 4.73AEE124 pKa = 4.72PKK126 pKa = 10.08QATPDD131 pKa = 3.44STDD134 pKa = 2.73IFTMPWNLLPSSWTVQRR151 pKa = 11.84AWSHH155 pKa = 5.48RR156 pKa = 11.84FVVKK160 pKa = 10.48RR161 pKa = 11.84KK162 pKa = 7.68WCVNLVSDD170 pKa = 4.56GKK172 pKa = 10.89KK173 pKa = 10.21VGSKK177 pKa = 10.46SVDD180 pKa = 2.87PRR182 pKa = 11.84YY183 pKa = 10.85NYY185 pKa = 10.08VAGKK189 pKa = 10.32NIVDD193 pKa = 3.37ASKK196 pKa = 9.76FCKK199 pKa = 9.94GLRR202 pKa = 11.84VTTEE206 pKa = 3.21WMNTGDD212 pKa = 5.0GKK214 pKa = 10.85IGDD217 pKa = 3.88IKK219 pKa = 10.84KK220 pKa = 10.24GALYY224 pKa = 10.65LITCTRR230 pKa = 11.84GGVSGDD236 pKa = 3.49SASLSFEE243 pKa = 4.14VVCAYY248 pKa = 8.49THH250 pKa = 6.21ACYY253 pKa = 9.89FKK255 pKa = 11.01SIGIQQ260 pKa = 3.05

Molecular weight: 29.16 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4

0

4

961

90

347

240.3

27.49

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.556 ± 0.252

2.185 ± 0.325

6.243 ± 0.105

5.099 ± 1.456

4.579 ± 0.717

4.787 ± 1.252

2.706 ± 0.666

5.203 ± 0.283

5.931 ± 1.284

7.076 ± 0.827

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.977 ± 0.357

4.266 ± 0.585

5.931 ± 0.789

3.122 ± 0.491

5.931 ± 0.314

7.908 ± 0.786

6.452 ± 0.131

6.764 ± 1.454

2.185 ± 0.399

5.099 ± 0.23
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski