Amino acid dipepetide frequency for Aspergillus nomiae NRRL 13137

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
8.275AlaAla: 8.275 ± 0.054
1.132AlaCys: 1.132 ± 0.013
4.092AlaAsp: 4.092 ± 0.027
4.856AlaGlu: 4.856 ± 0.034
3.208AlaPhe: 3.208 ± 0.024
5.621AlaGly: 5.621 ± 0.037
1.787AlaHis: 1.787 ± 0.021
4.418AlaIle: 4.418 ± 0.031
3.729AlaLys: 3.729 ± 0.027
7.872AlaLeu: 7.872 ± 0.043
1.968AlaMet: 1.968 ± 0.018
2.888AlaAsn: 2.888 ± 0.022
4.403AlaPro: 4.403 ± 0.04
3.31AlaGln: 3.31 ± 0.026
4.718AlaArg: 4.718 ± 0.031
6.998AlaSer: 6.998 ± 0.041
5.126AlaThr: 5.126 ± 0.032
5.472AlaVal: 5.472 ± 0.032
1.188AlaTrp: 1.188 ± 0.018
2.28AlaTyr: 2.28 ± 0.021
0.0AlaXaa: 0.0 ± 0.0
Cys
1.015CysAla: 1.015 ± 0.014
0.252CysCys: 0.252 ± 0.007
0.707CysAsp: 0.707 ± 0.011
0.625CysGlu: 0.625 ± 0.011
0.568CysPhe: 0.568 ± 0.011
0.945CysGly: 0.945 ± 0.014
0.36CysHis: 0.36 ± 0.009
0.787CysIle: 0.787 ± 0.012
0.497CysLys: 0.497 ± 0.01
1.407CysLeu: 1.407 ± 0.017
0.29CysMet: 0.29 ± 0.008
0.464CysAsn: 0.464 ± 0.01
0.68CysPro: 0.68 ± 0.013
0.487CysGln: 0.487 ± 0.008
0.818CysArg: 0.818 ± 0.013
0.998CysSer: 0.998 ± 0.013
0.743CysThr: 0.743 ± 0.012
0.854CysVal: 0.854 ± 0.014
0.214CysTrp: 0.214 ± 0.006
0.392CysTyr: 0.392 ± 0.009
0.0CysXaa: 0.0 ± 0.0
Asp
4.465AspAla: 4.465 ± 0.027
0.639AspCys: 0.639 ± 0.011
3.79AspAsp: 3.79 ± 0.037
4.107AspGlu: 4.107 ± 0.037
2.147AspPhe: 2.147 ± 0.02
3.927AspGly: 3.927 ± 0.031
1.291AspHis: 1.291 ± 0.013
3.228AspIle: 3.228 ± 0.023
2.24AspLys: 2.24 ± 0.024
5.173AspLeu: 5.173 ± 0.033
1.259AspMet: 1.259 ± 0.016
1.907AspAsn: 1.907 ± 0.019
3.36AspPro: 3.36 ± 0.025
1.944AspGln: 1.944 ± 0.02
3.077AspArg: 3.077 ± 0.025
4.121AspSer: 4.121 ± 0.031
3.074AspThr: 3.074 ± 0.022
3.657AspVal: 3.657 ± 0.026
0.896AspTrp: 0.896 ± 0.013
1.681AspTyr: 1.681 ± 0.018
0.0AspXaa: 0.0 ± 0.0
Glu
5.109GluAla: 5.109 ± 0.034
0.67GluCys: 0.67 ± 0.011
3.909GluAsp: 3.909 ± 0.036
5.158GluGlu: 5.158 ± 0.047
1.95GluPhe: 1.95 ± 0.018
3.593GluGly: 3.593 ± 0.029
1.411GluHis: 1.411 ± 0.016
3.091GluIle: 3.091 ± 0.023
3.529GluLys: 3.529 ± 0.029
5.224GluLeu: 5.224 ± 0.033
1.397GluMet: 1.397 ± 0.018
2.35GluAsn: 2.35 ± 0.024
2.767GluPro: 2.767 ± 0.04
2.527GluGln: 2.527 ± 0.025
3.79GluArg: 3.79 ± 0.03
4.352GluSer: 4.352 ± 0.034
3.523GluThr: 3.523 ± 0.027
3.53GluVal: 3.53 ± 0.024
0.898GluTrp: 0.898 ± 0.012
1.752GluTyr: 1.752 ± 0.018
0.0GluXaa: 0.0 ± 0.0
Phe
3.059PheAla: 3.059 ± 0.02
0.6PheCys: 0.6 ± 0.012
2.271PheAsp: 2.271 ± 0.021
2.084PheGlu: 2.084 ± 0.024
1.685PhePhe: 1.685 ± 0.021
2.858PheGly: 2.858 ± 0.024
0.987PheHis: 0.987 ± 0.015
1.952PheIle: 1.952 ± 0.019
1.446PheLys: 1.446 ± 0.017
3.753PheLeu: 3.753 ± 0.031
0.809PheMet: 0.809 ± 0.011
1.468PheAsn: 1.468 ± 0.015
2.046PhePro: 2.046 ± 0.021
1.473PheGln: 1.473 ± 0.017
2.016PheArg: 2.016 ± 0.023
3.065PheSer: 3.065 ± 0.024
2.244PheThr: 2.244 ± 0.02
2.464PheVal: 2.464 ± 0.022
0.684PheTrp: 0.684 ± 0.012
1.216PheTyr: 1.216 ± 0.017
0.0PheXaa: 0.0 ± 0.0
Gly
5.163GlyAla: 5.163 ± 0.037
0.943GlyCys: 0.943 ± 0.014
3.578GlyAsp: 3.578 ± 0.03
3.531GlyGlu: 3.531 ± 0.028
2.866GlyPhe: 2.866 ± 0.024
5.272GlyGly: 5.272 ± 0.041
1.694GlyHis: 1.694 ± 0.017
3.717GlyIle: 3.717 ± 0.027
3.272GlyLys: 3.272 ± 0.03
6.339GlyLeu: 6.339 ± 0.033
1.544GlyMet: 1.544 ± 0.018
2.549GlyAsn: 2.549 ± 0.022
3.295GlyPro: 3.295 ± 0.025
2.599GlyGln: 2.599 ± 0.022
3.938GlyArg: 3.938 ± 0.028
5.648GlySer: 5.648 ± 0.039
3.985GlyThr: 3.985 ± 0.026
4.548GlyVal: 4.548 ± 0.031
1.21GlyTrp: 1.21 ± 0.013
2.247GlyTyr: 2.247 ± 0.02
0.0GlyXaa: 0.0 ± 0.0
His
1.846HisAla: 1.846 ± 0.018
0.362HisCys: 0.362 ± 0.007
1.355HisAsp: 1.355 ± 0.017
1.347HisGlu: 1.347 ± 0.016
0.951HisPhe: 0.951 ± 0.013
1.75HisGly: 1.75 ± 0.018
0.862HisHis: 0.862 ± 0.014
1.322HisIle: 1.322 ± 0.017
0.854HisLys: 0.854 ± 0.013
2.337HisLeu: 2.337 ± 0.02
0.518HisMet: 0.518 ± 0.009
0.858HisAsn: 0.858 ± 0.013
1.716HisPro: 1.716 ± 0.019
0.967HisGln: 0.967 ± 0.015
1.598HisArg: 1.598 ± 0.017
1.922HisSer: 1.922 ± 0.022
1.318HisThr: 1.318 ± 0.017
1.461HisVal: 1.461 ± 0.016
0.379HisTrp: 0.379 ± 0.008
0.737HisTyr: 0.737 ± 0.011
0.0HisXaa: 0.0 ± 0.0
Ile
4.338IleAla: 4.338 ± 0.033
0.819IleCys: 0.819 ± 0.015
2.903IleAsp: 2.903 ± 0.023
2.855IleGlu: 2.855 ± 0.022
2.122IlePhe: 2.122 ± 0.024
3.402IleGly: 3.402 ± 0.027
1.307IleHis: 1.307 ± 0.015
2.662IleIle: 2.662 ± 0.027
2.045IleLys: 2.045 ± 0.019
4.909IleLeu: 4.909 ± 0.036
1.078IleMet: 1.078 ± 0.015
1.854IleAsn: 1.854 ± 0.021
3.253IlePro: 3.253 ± 0.023
2.032IleGln: 2.032 ± 0.021
2.852IleArg: 2.852 ± 0.021
4.058IleSer: 4.058 ± 0.027
2.918IleThr: 2.918 ± 0.026
3.393IleVal: 3.393 ± 0.029
0.761IleTrp: 0.761 ± 0.011
1.568IleTyr: 1.568 ± 0.018
0.0IleXaa: 0.0 ± 0.0
Lys
3.972LysAla: 3.972 ± 0.026
0.537LysCys: 0.537 ± 0.01
2.693LysAsp: 2.693 ± 0.023
3.239LysGlu: 3.239 ± 0.03
1.381LysPhe: 1.381 ± 0.016
2.881LysGly: 2.881 ± 0.025
1.076LysHis: 1.076 ± 0.014
2.141LysIle: 2.141 ± 0.019
2.851LysLys: 2.851 ± 0.038
3.955LysLeu: 3.955 ± 0.027
0.923LysMet: 0.923 ± 0.012
1.649LysAsn: 1.649 ± 0.019
2.542LysPro: 2.542 ± 0.025
1.811LysGln: 1.811 ± 0.02
3.139LysArg: 3.139 ± 0.029
3.314LysSer: 3.314 ± 0.025
2.614LysThr: 2.614 ± 0.022
2.772LysVal: 2.772 ± 0.024
0.657LysTrp: 0.657 ± 0.011
1.385LysTyr: 1.385 ± 0.014
0.0LysXaa: 0.0 ± 0.0
Leu
7.91LeuAla: 7.91 ± 0.039
1.32LeuCys: 1.32 ± 0.017
5.251LeuAsp: 5.251 ± 0.035
5.588LeuGlu: 5.588 ± 0.035
3.556LeuPhe: 3.556 ± 0.029
6.186LeuGly: 6.186 ± 0.036
2.366LeuHis: 2.366 ± 0.021
4.221LeuIle: 4.221 ± 0.029
4.087LeuLys: 4.087 ± 0.03
8.968LeuLeu: 8.968 ± 0.048
1.85LeuMet: 1.85 ± 0.018
3.313LeuAsn: 3.313 ± 0.024
5.594LeuPro: 5.594 ± 0.032
4.002LeuGln: 4.002 ± 0.029
5.867LeuArg: 5.867 ± 0.035
7.79LeuSer: 7.79 ± 0.048
4.972LeuThr: 4.972 ± 0.029
5.721LeuVal: 5.721 ± 0.034
1.263LeuTrp: 1.263 ± 0.015
2.598LeuTyr: 2.598 ± 0.025
0.0LeuXaa: 0.0 ± 0.0
Met
2.166MetAla: 2.166 ± 0.022
0.266MetCys: 0.266 ± 0.007
1.238MetAsp: 1.238 ± 0.015
1.298MetGlu: 1.298 ± 0.013
0.751MetPhe: 0.751 ± 0.012
1.48MetGly: 1.48 ± 0.017
0.483MetHis: 0.483 ± 0.009
1.044MetIle: 1.044 ± 0.014
0.981MetLys: 0.981 ± 0.012
1.92MetLeu: 1.92 ± 0.019
0.554MetMet: 0.554 ± 0.01
0.81MetAsn: 0.81 ± 0.014
1.175MetPro: 1.175 ± 0.014
0.862MetGln: 0.862 ± 0.011
1.24MetArg: 1.24 ± 0.014
1.858MetSer: 1.858 ± 0.017
1.304MetThr: 1.304 ± 0.016
1.393MetVal: 1.393 ± 0.016
0.265MetTrp: 0.265 ± 0.006
0.55MetTyr: 0.55 ± 0.009
0.0MetXaa: 0.0 ± 0.0
Asn
3.101AsnAla: 3.101 ± 0.023
0.485AsnCys: 0.485 ± 0.009
1.962AsnAsp: 1.962 ± 0.019
2.044AsnGlu: 2.044 ± 0.019
1.355AsnPhe: 1.355 ± 0.016
2.93AsnGly: 2.93 ± 0.025
0.87AsnHis: 0.87 ± 0.013
2.146AsnIle: 2.146 ± 0.019
1.483AsnLys: 1.483 ± 0.017
3.281AsnLeu: 3.281 ± 0.026
0.863AsnMet: 0.863 ± 0.011
1.455AsnAsn: 1.455 ± 0.017
2.49AsnPro: 2.49 ± 0.019
1.337AsnGln: 1.337 ± 0.016
1.958AsnArg: 1.958 ± 0.018
2.717AsnSer: 2.717 ± 0.024
2.228AsnThr: 2.228 ± 0.018
2.393AsnVal: 2.393 ± 0.021
0.587AsnTrp: 0.587 ± 0.01
1.11AsnTyr: 1.11 ± 0.015
0.0AsnXaa: 0.0 ± 0.0
Pro
4.752ProAla: 4.752 ± 0.04
0.573ProCys: 0.573 ± 0.009
3.215ProAsp: 3.215 ± 0.025
3.898ProGlu: 3.898 ± 0.038
2.172ProPhe: 2.172 ± 0.021
3.883ProGly: 3.883 ± 0.031
1.32ProHis: 1.32 ± 0.016
2.559ProIle: 2.559 ± 0.021
2.53ProLys: 2.53 ± 0.027
4.823ProLeu: 4.823 ± 0.032
1.059ProMet: 1.059 ± 0.016
2.176ProAsn: 2.176 ± 0.023
4.449ProPro: 4.449 ± 0.057
2.408ProGln: 2.408 ± 0.025
3.319ProArg: 3.319 ± 0.027
5.927ProSer: 5.927 ± 0.042
3.892ProThr: 3.892 ± 0.034
3.684ProVal: 3.684 ± 0.029
0.802ProTrp: 0.802 ± 0.013
1.555ProTyr: 1.555 ± 0.017
0.0ProXaa: 0.0 ± 0.0
Gln
3.352GlnAla: 3.352 ± 0.028
0.482GlnCys: 0.482 ± 0.01
2.07GlnAsp: 2.07 ± 0.018
2.481GlnGlu: 2.481 ± 0.021
1.341GlnPhe: 1.341 ± 0.014
2.492GlnGly: 2.492 ± 0.018
1.047GlnHis: 1.047 ± 0.017
1.949GlnIle: 1.949 ± 0.017
1.982GlnLys: 1.982 ± 0.018
3.593GlnLeu: 3.593 ± 0.029
0.885GlnMet: 0.885 ± 0.013
1.591GlnAsn: 1.591 ± 0.017
2.486GlnPro: 2.486 ± 0.027
2.242GlnGln: 2.242 ± 0.039
2.63GlnArg: 2.63 ± 0.022
3.238GlnSer: 3.238 ± 0.024
2.369GlnThr: 2.369 ± 0.019
2.247GlnVal: 2.247 ± 0.022
0.617GlnTrp: 0.617 ± 0.011
1.23GlnTyr: 1.23 ± 0.014
0.0GlnXaa: 0.0 ± 0.0
Arg
4.501ArgAla: 4.501 ± 0.027
0.743ArgCys: 0.743 ± 0.013
3.269ArgAsp: 3.269 ± 0.027
3.706ArgGlu: 3.706 ± 0.031
2.219ArgPhe: 2.219 ± 0.02
3.557ArgGly: 3.557 ± 0.028
1.556ArgHis: 1.556 ± 0.018
2.929ArgIle: 2.929 ± 0.022
3.227ArgLys: 3.227 ± 0.027
5.687ArgLeu: 5.687 ± 0.035
1.289ArgMet: 1.289 ± 0.017
2.238ArgAsn: 2.238 ± 0.019
3.369ArgPro: 3.369 ± 0.032
2.583ArgGln: 2.583 ± 0.024
4.872ArgArg: 4.872 ± 0.038
4.745ArgSer: 4.745 ± 0.035
3.234ArgThr: 3.234 ± 0.026
3.478ArgVal: 3.478 ± 0.024
0.99ArgTrp: 0.99 ± 0.013
1.771ArgTyr: 1.771 ± 0.019
0.0ArgXaa: 0.0 ± 0.0
Ser
6.493SerAla: 6.493 ± 0.034
0.953SerCys: 0.953 ± 0.013
4.298SerAsp: 4.298 ± 0.031
4.237SerGlu: 4.237 ± 0.028
3.178SerPhe: 3.178 ± 0.026
5.532SerGly: 5.532 ± 0.036
2.031SerHis: 2.031 ± 0.022
4.177SerIle: 4.177 ± 0.028
3.586SerLys: 3.586 ± 0.026
7.622SerLeu: 7.622 ± 0.04
1.719SerMet: 1.719 ± 0.019
2.983SerAsn: 2.983 ± 0.026
5.286SerPro: 5.286 ± 0.039
3.384SerGln: 3.384 ± 0.026
4.9SerArg: 4.9 ± 0.038
8.574SerSer: 8.574 ± 0.059
5.539SerThr: 5.539 ± 0.036
4.811SerVal: 4.811 ± 0.031
1.199SerTrp: 1.199 ± 0.015
2.171SerTyr: 2.171 ± 0.022
0.0SerXaa: 0.0 ± 0.0
Thr
5.133ThrAla: 5.133 ± 0.03
0.786ThrCys: 0.786 ± 0.011
2.947ThrAsp: 2.947 ± 0.026
3.22ThrGlu: 3.22 ± 0.026
2.291ThrPhe: 2.291 ± 0.023
4.29ThrGly: 4.29 ± 0.029
1.332ThrHis: 1.332 ± 0.016
3.173ThrIle: 3.173 ± 0.024
2.48ThrLys: 2.48 ± 0.022
5.376ThrLeu: 5.376 ± 0.033
1.253ThrMet: 1.253 ± 0.016
2.091ThrAsn: 2.091 ± 0.019
4.159ThrPro: 4.159 ± 0.034
2.094ThrGln: 2.094 ± 0.018
3.038ThrArg: 3.038 ± 0.022
5.19ThrSer: 5.19 ± 0.033
4.125ThrThr: 4.125 ± 0.039
3.975ThrVal: 3.975 ± 0.033
0.898ThrTrp: 0.898 ± 0.013
1.697ThrTyr: 1.697 ± 0.018
0.0ThrXaa: 0.0 ± 0.0
Val
5.275ValAla: 5.275 ± 0.035
0.892ValCys: 0.892 ± 0.013
3.815ValAsp: 3.815 ± 0.028
3.789ValGlu: 3.789 ± 0.031
2.589ValPhe: 2.589 ± 0.023
4.159ValGly: 4.159 ± 0.031
1.498ValHis: 1.498 ± 0.016
3.194ValIle: 3.194 ± 0.025
2.796ValLys: 2.796 ± 0.024
5.849ValLeu: 5.849 ± 0.033
1.353ValMet: 1.353 ± 0.016
2.312ValAsn: 2.312 ± 0.023
3.683ValPro: 3.683 ± 0.027
2.49ValGln: 2.49 ± 0.02
3.474ValArg: 3.474 ± 0.025
4.919ValSer: 4.919 ± 0.032
3.652ValThr: 3.652 ± 0.031
4.404ValVal: 4.404 ± 0.03
0.908ValTrp: 0.908 ± 0.013
1.893ValTyr: 1.893 ± 0.019
0.0ValXaa: 0.0 ± 0.0
Trp
1.175TrpAla: 1.175 ± 0.016
0.209TrpCys: 0.209 ± 0.006
0.927TrpAsp: 0.927 ± 0.014
0.876TrpGlu: 0.876 ± 0.012
0.578TrpPhe: 0.578 ± 0.01
0.98TrpGly: 0.98 ± 0.012
0.377TrpHis: 0.377 ± 0.009
0.827TrpIle: 0.827 ± 0.013
0.833TrpLys: 0.833 ± 0.012
1.449TrpLeu: 1.449 ± 0.014
0.382TrpMet: 0.382 ± 0.008
0.666TrpAsn: 0.666 ± 0.012
0.628TrpPro: 0.628 ± 0.012
0.585TrpGln: 0.585 ± 0.01
0.979TrpArg: 0.979 ± 0.012
1.075TrpSer: 1.075 ± 0.015
0.953TrpThr: 0.953 ± 0.015
0.94TrpVal: 0.94 ± 0.013
0.293TrpTrp: 0.293 ± 0.007
0.462TrpTyr: 0.462 ± 0.01
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.239TyrAla: 2.239 ± 0.022
0.441TyrCys: 0.441 ± 0.011
1.694TyrAsp: 1.694 ± 0.016
1.591TyrGlu: 1.591 ± 0.018
1.273TyrPhe: 1.273 ± 0.017
2.198TyrGly: 2.198 ± 0.025
0.803TyrHis: 0.803 ± 0.014
1.589TyrIle: 1.589 ± 0.019
1.086TyrLys: 1.086 ± 0.015
2.884TyrLeu: 2.884 ± 0.021
0.645TyrMet: 0.645 ± 0.009
1.173TyrAsn: 1.173 ± 0.016
1.617TyrPro: 1.617 ± 0.016
1.189TyrGln: 1.189 ± 0.015
1.739TyrArg: 1.739 ± 0.018
2.166TyrSer: 2.166 ± 0.02
1.737TyrThr: 1.737 ± 0.019
1.747TyrVal: 1.747 ± 0.017
0.486TyrTrp: 0.486 ± 0.009
1.009TyrTyr: 1.009 ± 0.013
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 11914 proteins (5973996 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski