Cytophagaceae bacterium

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Cytophagia; Cytophagales; Cytophagaceae; unclassified Cytophagaceae

Average proteome isoelectric point is 6.75

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1132 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4Q3JNA4|A0A4Q3JNA4_9BACT SusC/RagA family TonB-linked outer membrane protein (Fragment) OS=Cytophagaceae bacterium OX=2026729 GN=EOO37_01905 PE=4 SV=1
MM1 pKa = 7.56TNFTSWGHH9 pKa = 4.76TEE11 pKa = 4.33EE12 pKa = 4.24PTVTQFEE19 pKa = 4.31QQLGFKK25 pKa = 10.59LPTDD29 pKa = 3.6YY30 pKa = 11.21RR31 pKa = 11.84SFLLQNNGGSFKK43 pKa = 10.75KK44 pKa = 10.29QVFFVDD50 pKa = 4.87DD51 pKa = 4.76LKK53 pKa = 11.21QDD55 pKa = 2.84IMLGVLYY62 pKa = 10.55GITNEE67 pKa = 4.12KK68 pKa = 10.75SVDD71 pKa = 3.53LTIASWMEE79 pKa = 3.81EE80 pKa = 4.15YY81 pKa = 10.95GDD83 pKa = 4.06EE84 pKa = 4.19LQEE87 pKa = 4.23DD88 pKa = 4.36ALIIGADD95 pKa = 3.61PGGGMLLYY103 pKa = 9.25ITAGEE108 pKa = 4.22DD109 pKa = 2.88MGIYY113 pKa = 9.96YY114 pKa = 9.32WDD116 pKa = 3.69HH117 pKa = 6.42AHH119 pKa = 6.86FFSQSSEE126 pKa = 4.36EE127 pKa = 3.98EE128 pKa = 4.18GNTYY132 pKa = 10.02FVAPSFAAFCALLTDD147 pKa = 4.16YY148 pKa = 11.16KK149 pKa = 10.68PVASS153 pKa = 4.25

Molecular weight: 17.2 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4Q3JNH5|A0A4Q3JNH5_9BACT Uncharacterized protein OS=Cytophagaceae bacterium OX=2026729 GN=EOO37_05115 PE=4 SV=1
MM1 pKa = 7.64PAYY4 pKa = 10.67SLDD7 pKa = 3.09LRR9 pKa = 11.84TRR11 pKa = 11.84VVAAYY16 pKa = 7.41RR17 pKa = 11.84QPGARR22 pKa = 11.84QATVAALFGVSVSFLKK38 pKa = 10.79KK39 pKa = 10.06LLRR42 pKa = 11.84HH43 pKa = 5.46EE44 pKa = 4.38RR45 pKa = 11.84QTGSLAPKK53 pKa = 8.52PASGGRR59 pKa = 11.84ARR61 pKa = 11.84YY62 pKa = 9.6LDD64 pKa = 4.28AEE66 pKa = 4.18AQAWLVAFMGQQPDD80 pKa = 3.17ATLAEE85 pKa = 5.12LSQAWQAQGGRR96 pKa = 11.84PVGQTCLWQVLDD108 pKa = 3.53EE109 pKa = 4.61HH110 pKa = 6.77KK111 pKa = 10.69LRR113 pKa = 11.84RR114 pKa = 11.84KK115 pKa = 9.59KK116 pKa = 10.67KK117 pKa = 8.21PARR120 pKa = 3.75

Molecular weight: 13.25 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1132

0

1132

216817

20

839

191.5

21.32

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.821 ± 0.099

0.801 ± 0.025

5.165 ± 0.055

5.803 ± 0.083

4.495 ± 0.065

6.895 ± 0.08

2.217 ± 0.047

5.197 ± 0.067

5.17 ± 0.077

10.924 ± 0.091

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.044 ± 0.032

3.857 ± 0.064

4.573 ± 0.052

4.541 ± 0.062

5.537 ± 0.062

5.477 ± 0.068

5.857 ± 0.065

6.831 ± 0.066

1.133 ± 0.034

3.66 ± 0.056
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski