Nitrososphaera phage Pro-Nvie1

Taxonomy: Viruses; unclassified bacterial viruses

Average proteome isoelectric point is 5.85

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 30 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|F8K9P7|F8K9P7_9VIRU Uncharacterized protein nvie-Pro1-28 OS=Nitrososphaera phage Pro-Nvie1 OX=927392 GN=nvie-Pro1-28 PE=4 SV=1
MM1 pKa = 7.67RR2 pKa = 11.84KK3 pKa = 9.18IPGLDD8 pKa = 3.52NIVKK12 pKa = 10.45DD13 pKa = 3.9LAEE16 pKa = 4.26KK17 pKa = 10.35QNRR20 pKa = 11.84AQAEE24 pKa = 4.16DD25 pKa = 4.37GIEE28 pKa = 4.19DD29 pKa = 3.54LTLFRR34 pKa = 11.84QNRR37 pKa = 11.84VDD39 pKa = 3.44TDD41 pKa = 4.31AITYY45 pKa = 10.8ADD47 pKa = 4.2TEE49 pKa = 4.39SHH51 pKa = 6.49AANGDD56 pKa = 3.4RR57 pKa = 11.84TDD59 pKa = 3.76ADD61 pKa = 4.7GISLADD67 pKa = 3.63VEE69 pKa = 4.61TALAQNRR76 pKa = 11.84QQFDD80 pKa = 3.32ADD82 pKa = 3.42GDD84 pKa = 4.08FFCGSGAVINEE95 pKa = 3.83TDD97 pKa = 3.41KK98 pKa = 11.43PSGAFAGGIGNFNEE112 pKa = 4.06PDD114 pKa = 3.35QPAVADD120 pKa = 4.69FSDD123 pKa = 3.53AGG125 pKa = 3.58

Molecular weight: 13.28 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|F8K9N1|F8K9N1_9VIRU Uncharacterized protein nvie-Pro1-10 OS=Nitrososphaera phage Pro-Nvie1 OX=927392 GN=nvie-Pro1-10 PE=4 SV=1
MM1 pKa = 7.95ADD3 pKa = 3.89LKK5 pKa = 11.37SIFDD9 pKa = 3.36KK10 pKa = 11.02YY11 pKa = 10.31IRR13 pKa = 11.84EE14 pKa = 4.49GEE16 pKa = 4.22SGRR19 pKa = 11.84SNTNEE24 pKa = 3.83LLKK27 pKa = 11.01AIVDD31 pKa = 4.2EE32 pKa = 4.71IEE34 pKa = 3.91ALKK37 pKa = 10.67SKK39 pKa = 10.55RR40 pKa = 11.84RR41 pKa = 3.64

Molecular weight: 4.73 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

30

0

30

6061

41

594

202.0

22.42

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.701 ± 0.767

1.039 ± 0.285

6.418 ± 0.331

6.583 ± 0.375

3.663 ± 0.321

6.715 ± 0.45

1.65 ± 0.259

5.346 ± 0.334

5.643 ± 0.496

8.282 ± 0.402

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.475 ± 0.339

4.389 ± 0.345

3.696 ± 0.297

4.389 ± 0.356

5.89 ± 0.368

7.078 ± 0.387

6.138 ± 0.457

6.55 ± 0.399

1.006 ± 0.157

3.349 ± 0.269
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski