Rhodobacter phage RcRhea

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Cronusvirus; Rhodobacter virus RcCronus

Average proteome isoelectric point is 6.52

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 45 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0K1LLZ8|A0A0K1LLZ8_9CAUD Tail protein OS=Rhodobacter phage RcRhea OX=1662332 GN=RCRHEA_14 PE=4 SV=1
MM1 pKa = 7.76IGGDD5 pKa = 3.24DD6 pKa = 3.17WGVFFDD12 pKa = 5.18PDD14 pKa = 3.85DD15 pKa = 4.43FADD18 pKa = 3.69SAVWDD23 pKa = 4.1TQAGEE28 pKa = 4.09FVEE31 pKa = 3.97IDD33 pKa = 3.98GIFEE37 pKa = 4.39AGRR40 pKa = 11.84EE41 pKa = 4.05VVLAGDD47 pKa = 3.88GAGISAIMPVLTVASDD63 pKa = 3.85SVPATAAQGDD73 pKa = 4.16DD74 pKa = 4.26LEE76 pKa = 4.96VRR78 pKa = 11.84DD79 pKa = 4.07KK80 pKa = 11.13NFRR83 pKa = 11.84VADD86 pKa = 4.28LQPDD90 pKa = 4.15GSGLTRR96 pKa = 11.84VILEE100 pKa = 4.23RR101 pKa = 11.84VV102 pKa = 3.26

Molecular weight: 10.76 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0K1LM45|A0A0K1LM45_9CAUD Terminase small subunit OS=Rhodobacter phage RcRhea OX=1662332 GN=RCRHEA_1 PE=4 SV=1
MM1 pKa = 7.22MMNSDD6 pKa = 4.56FEE8 pKa = 4.33AATIRR13 pKa = 11.84NGLDD17 pKa = 2.7VDD19 pKa = 4.15VILRR23 pKa = 11.84RR24 pKa = 11.84RR25 pKa = 11.84GLPILDD31 pKa = 4.21HH32 pKa = 6.79LNAAEE37 pKa = 3.92RR38 pKa = 11.84RR39 pKa = 11.84TVEE42 pKa = 3.95TYY44 pKa = 10.53SAIFEE49 pKa = 4.11AVAAGGAVMPRR60 pKa = 11.84DD61 pKa = 3.52RR62 pKa = 11.84LAGGGGGGPSGPSRR76 pKa = 11.84EE77 pKa = 4.22GRR79 pKa = 11.84QSRR82 pKa = 11.84AVDD85 pKa = 3.2QAAFLRR91 pKa = 11.84AMGAAISARR100 pKa = 11.84PVLVFGKK107 pKa = 10.06RR108 pKa = 11.84NPVEE112 pKa = 4.02VGPLRR117 pKa = 11.84FWHH120 pKa = 6.32SFTVDD125 pKa = 3.05GLSVRR130 pKa = 11.84GALEE134 pKa = 3.71RR135 pKa = 11.84FGVKK139 pKa = 10.11RR140 pKa = 11.84GPIVNAAVVAEE151 pKa = 4.52VKK153 pKa = 10.89AMAAAVAAAAAVTRR167 pKa = 11.84NPLAEE172 pKa = 3.94

Molecular weight: 18.0 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

45

0

45

11330

35

1301

251.8

26.92

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.395 ± 0.57

0.715 ± 0.122

6.408 ± 0.24

5.322 ± 0.326

3.583 ± 0.201

9.965 ± 0.518

1.695 ± 0.241

3.751 ± 0.175

3.38 ± 0.327

8.288 ± 0.211

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.021 ± 0.1

2.427 ± 0.204

5.905 ± 0.334

2.798 ± 0.171

7.396 ± 0.441

5.049 ± 0.311

5.455 ± 0.434

7.741 ± 0.257

1.968 ± 0.146

1.739 ± 0.185
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski