Halomonas sp. A11-A

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Oceanospirillales; Halomonadaceae; Halomonas; unclassified Halomonas

Average proteome isoelectric point is 6.21

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3109 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A317NLK3|A0A317NLK3_9GAMM Amino acid ABC transporter substrate-binding protein (PAAT family) OS=Halomonas sp. A11-A OX=2183985 GN=DER72_10938 PE=3 SV=1
MM1 pKa = 7.62ILFPPLKK8 pKa = 8.91TLAAAVLVATSGFAVSSAQAEE29 pKa = 4.21DD30 pKa = 4.6FYY32 pKa = 11.47SGKK35 pKa = 7.74TLEE38 pKa = 4.35VVVPFGEE45 pKa = 4.9GGATYY50 pKa = 10.49VAAKK54 pKa = 9.94FLEE57 pKa = 4.57PFLEE61 pKa = 4.05KK62 pKa = 10.34HH63 pKa = 6.16LPGNPQVNVRR73 pKa = 11.84TRR75 pKa = 11.84PGGGSILGANWFQQNAEE92 pKa = 4.26PDD94 pKa = 3.64GEE96 pKa = 4.6TILFTTSSTSNPYY109 pKa = 9.55VLGMDD114 pKa = 3.21AVEE117 pKa = 4.2YY118 pKa = 10.54DD119 pKa = 4.01LAAMRR124 pKa = 11.84PAYY127 pKa = 9.87SLPFGAVIYY136 pKa = 8.44VSPRR140 pKa = 11.84TGIEE144 pKa = 3.76SSADD148 pKa = 3.22LHH150 pKa = 8.04SPDD153 pKa = 3.18TTLIYY158 pKa = 10.8GGIAAAASDD167 pKa = 4.63LPVLLAFEE175 pKa = 4.88LLEE178 pKa = 5.27LDD180 pKa = 3.7LRR182 pKa = 11.84AVLGFDD188 pKa = 3.19GRR190 pKa = 11.84GPARR194 pKa = 11.84LAFEE198 pKa = 4.57RR199 pKa = 11.84GEE201 pKa = 4.29TNIDD205 pKa = 3.65FQFTPAYY212 pKa = 8.04MSQVVDD218 pKa = 3.67MVEE221 pKa = 4.04AGSAVPIMSAGSVGDD236 pKa = 4.26DD237 pKa = 3.18GVLSARR243 pKa = 11.84DD244 pKa = 3.76LAFPDD249 pKa = 3.93YY250 pKa = 8.27PTVYY254 pKa = 9.81EE255 pKa = 4.52VYY257 pKa = 10.06EE258 pKa = 4.08EE259 pKa = 5.23LYY261 pKa = 10.88GEE263 pKa = 4.46PPSGVEE269 pKa = 3.15WDD271 pKa = 3.29AFQAIGATTFNFGLTAYY288 pKa = 9.89LPEE291 pKa = 4.42GTPDD295 pKa = 3.16EE296 pKa = 4.21VVEE299 pKa = 4.59IFEE302 pKa = 4.07QTIAAINDD310 pKa = 3.49DD311 pKa = 4.29PDD313 pKa = 3.68YY314 pKa = 11.39QEE316 pKa = 4.29QSQDD320 pKa = 3.41AIGGYY325 pKa = 10.43DD326 pKa = 4.68LLPASAVTDD335 pKa = 3.82SLSEE339 pKa = 3.9ALQPSDD345 pKa = 3.64EE346 pKa = 4.21VRR348 pKa = 11.84DD349 pKa = 3.83YY350 pKa = 11.71LRR352 pKa = 11.84TLLSEE357 pKa = 4.47KK358 pKa = 10.74YY359 pKa = 10.18DD360 pKa = 3.6VEE362 pKa = 4.29LL363 pKa = 5.22

Molecular weight: 38.85 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A317NU69|A0A317NU69_9GAMM ATP synthase epsilon chain OS=Halomonas sp. A11-A OX=2183985 GN=atpC PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.51RR3 pKa = 11.84TFQPSVLKK11 pKa = 10.34RR12 pKa = 11.84KK13 pKa = 9.1RR14 pKa = 11.84VHH16 pKa = 6.26GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.3NGRR28 pKa = 11.84AVLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.93GRR39 pKa = 11.84KK40 pKa = 8.85RR41 pKa = 11.84LSAA44 pKa = 3.96

Molecular weight: 5.15 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3109

0

3109

993874

26

1660

319.7

35.02

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.99 ± 0.061

0.888 ± 0.015

5.452 ± 0.039

7.045 ± 0.049

3.346 ± 0.028

8.381 ± 0.036

2.415 ± 0.02

4.38 ± 0.034

2.506 ± 0.036

11.845 ± 0.062

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.39 ± 0.024

2.254 ± 0.022

5.18 ± 0.037

3.587 ± 0.026

7.878 ± 0.048

4.92 ± 0.027

4.68 ± 0.028

7.225 ± 0.038

1.458 ± 0.021

2.179 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski