Bifidobacteriaceae bacterium NR015

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Bifidobacteriales; Bifidobacteriaceae; unclassified Bifidobacteriaceae (miscellaneous)

Average proteome isoelectric point is 6.42

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1751 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3E2CVS9|A0A3E2CVS9_9BIFI Multidrug DMT transporter permease OS=Bifidobacteriaceae bacterium NR015 OX=2021362 GN=CG399_06355 PE=4 SV=1
MM1 pKa = 7.9PYY3 pKa = 10.33VIAEE7 pKa = 4.09PCVDD11 pKa = 4.1VKK13 pKa = 11.38DD14 pKa = 3.94KK15 pKa = 11.56ACVDD19 pKa = 4.0EE20 pKa = 5.6CPVDD24 pKa = 4.37CIYY27 pKa = 11.15EE28 pKa = 4.18GDD30 pKa = 3.46RR31 pKa = 11.84TLYY34 pKa = 10.34INPNEE39 pKa = 4.32CVDD42 pKa = 4.51CGACEE47 pKa = 3.78PACPVEE53 pKa = 4.47AIFYY57 pKa = 10.5EE58 pKa = 4.91DD59 pKa = 5.32DD60 pKa = 5.09LPEE63 pKa = 4.41DD64 pKa = 3.78WEE66 pKa = 4.48WYY68 pKa = 9.7RR69 pKa = 11.84DD70 pKa = 3.48AAVDD74 pKa = 3.88YY75 pKa = 10.47FDD77 pKa = 5.63KK78 pKa = 11.47LGDD81 pKa = 3.74LGGATDD87 pKa = 4.59AGASGWDD94 pKa = 3.71EE95 pKa = 4.03ARR97 pKa = 11.84VAALPSRR104 pKa = 11.84SDD106 pKa = 3.23KK107 pKa = 11.25ASSS110 pKa = 3.01

Molecular weight: 12.02 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3E2CUN5|A0A3E2CUN5_9BIFI RNA methyltransferase OS=Bifidobacteriaceae bacterium NR015 OX=2021362 GN=CG399_06635 PE=4 SV=1
MM1 pKa = 7.44KK2 pKa = 9.6RR3 pKa = 11.84TFQPNNRR10 pKa = 11.84RR11 pKa = 11.84RR12 pKa = 11.84HH13 pKa = 4.83MKK15 pKa = 9.27HH16 pKa = 5.56GFRR19 pKa = 11.84VRR21 pKa = 11.84MRR23 pKa = 11.84TRR25 pKa = 11.84AGRR28 pKa = 11.84AVINRR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.06GRR39 pKa = 11.84KK40 pKa = 8.87SLSAA44 pKa = 3.86

Molecular weight: 5.35 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1751

0

1751

397942

23

2183

227.3

25.05

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.681 ± 0.067

1.102 ± 0.029

6.084 ± 0.076

6.055 ± 0.064

3.583 ± 0.046

6.757 ± 0.063

2.111 ± 0.031

6.194 ± 0.061

5.702 ± 0.079

8.762 ± 0.073

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.482 ± 0.031

4.748 ± 0.06

3.964 ± 0.04

3.538 ± 0.044

5.072 ± 0.064

7.113 ± 0.071

5.488 ± 0.045

7.508 ± 0.06

1.157 ± 0.024

2.899 ± 0.047
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski