Herbaspirillum sp. YR522

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales; Oxalobacteraceae; Herbaspirillum; unclassified Herbaspirillum

Average proteome isoelectric point is 6.89

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4612 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|J2W9X1|J2W9X1_9BURK Putative pterin-4-alpha-carbinolamine dehydratase OS=Herbaspirillum sp. YR522 OX=1144342 GN=PMI40_00523 PE=3 SV=1
MM1 pKa = 7.15ATAPSPQANPGDD13 pKa = 4.23KK14 pKa = 9.18VTGPVRR20 pKa = 11.84VVMPDD25 pKa = 3.02QGGSFQLNAPRR36 pKa = 11.84GAVQKK41 pKa = 10.17VQVVDD46 pKa = 3.47VDD48 pKa = 4.73LVLHH52 pKa = 6.11MADD55 pKa = 3.19GTKK58 pKa = 10.33VVLAGGAMEE67 pKa = 5.75AMDD70 pKa = 5.38DD71 pKa = 3.49KK72 pKa = 11.73SKK74 pKa = 11.46VKK76 pKa = 10.54FSDD79 pKa = 3.62GSADD83 pKa = 3.29TGRR86 pKa = 11.84LLDD89 pKa = 3.9TVGKK93 pKa = 10.39IPLAPNDD100 pKa = 3.3QSRR103 pKa = 11.84VLNSDD108 pKa = 3.37PVAAQDD114 pKa = 3.48RR115 pKa = 11.84TGTTEE120 pKa = 3.64QYY122 pKa = 11.41GPGQGQGADD131 pKa = 3.14RR132 pKa = 11.84NAAPVASDD140 pKa = 3.9TLSQLAKK147 pKa = 10.5VIADD151 pKa = 3.51NSHH154 pKa = 6.38SVTGDD159 pKa = 3.0ATSQLAKK166 pKa = 10.72VIADD170 pKa = 3.41NSKK173 pKa = 10.77SVTNGAQTLSIPTQPTAGDD192 pKa = 3.71ASPVGGQVATNPNQNSSDD210 pKa = 4.04SEE212 pKa = 3.96PTRR215 pKa = 11.84PVDD218 pKa = 3.45VQVPPEE224 pKa = 4.38RR225 pKa = 11.84PPTTPSTVVTGPGSPTLSVNLVNLTTITQVGDD257 pKa = 3.31VLYY260 pKa = 10.74GSGGTPQSASDD271 pKa = 3.82GSNAVQFARR280 pKa = 11.84QVITAGDD287 pKa = 4.05DD288 pKa = 3.14VHH290 pKa = 6.89TIYY293 pKa = 11.18ASGNNSNTFIKK304 pKa = 10.37VFNVKK309 pKa = 10.13VGGDD313 pKa = 3.57VTLLTLTVMGVPSDD327 pKa = 3.69VTLLNGTNMGGGVYY341 pKa = 10.02QITIAPGQTEE351 pKa = 4.26FNLQFQYY358 pKa = 9.52NTQLANASSPVHH370 pKa = 5.71SQFDD374 pKa = 3.82LSFVTTMATSSGVLTLSDD392 pKa = 3.55TRR394 pKa = 11.84HH395 pKa = 5.07VAIKK399 pKa = 10.39DD400 pKa = 3.58ATSSADD406 pKa = 3.56LTYY409 pKa = 10.78LDD411 pKa = 5.36PSTGDD416 pKa = 3.16NVLIFPAQGVPHH428 pKa = 6.7EE429 pKa = 4.23VHH431 pKa = 6.59AGNGGVTIYY440 pKa = 10.95GSNANDD446 pKa = 3.94LLYY449 pKa = 10.99GGTGNDD455 pKa = 3.28IIYY458 pKa = 10.27GGVGSSSFFQGGAGADD474 pKa = 3.47QLVGSSTGNNTASYY488 pKa = 10.3AGSTQGVTIDD498 pKa = 4.14LNTGVGTGGDD508 pKa = 3.53AQGDD512 pKa = 3.86QLTNIQNLVGSANNDD527 pKa = 3.21TFVASGQVNRR537 pKa = 11.84FDD539 pKa = 4.19GGTGGSDD546 pKa = 3.15TVSYY550 pKa = 10.48AHH552 pKa = 6.6SASGVTVDD560 pKa = 4.46LVNNVGSGGDD570 pKa = 3.57AAGDD574 pKa = 3.6TYY576 pKa = 11.72VSIEE580 pKa = 4.01NVIGSTSNDD589 pKa = 2.96TFIASSAANSFTGNGGTDD607 pKa = 3.22TVSYY611 pKa = 10.55ALSTAGVTVNLKK623 pKa = 10.88SNLGSGGFAAGDD635 pKa = 3.86TYY637 pKa = 11.69NGIQNFVGSAYY648 pKa = 10.59DD649 pKa = 3.44DD650 pKa = 3.81TFVGNAAVNSFDD662 pKa = 4.34GGSGGNDD669 pKa = 2.89TVSYY673 pKa = 10.26AGSTAAVTVNLVTGRR688 pKa = 11.84GSNGDD693 pKa = 3.68AAGDD697 pKa = 3.64TYY699 pKa = 11.29IRR701 pKa = 11.84IQNVIGGDD709 pKa = 3.7GNDD712 pKa = 3.55TFVVGLDD719 pKa = 3.33SKK721 pKa = 11.31NFNGGGGTLNTVDD734 pKa = 3.91YY735 pKa = 9.84SGSSSAITANMLTNTVTRR753 pKa = 11.84TFEE756 pKa = 4.04PVNALTNIQKK766 pKa = 9.92IVGSSYY772 pKa = 10.85SDD774 pKa = 3.34TFIGGAGADD783 pKa = 3.39IFDD786 pKa = 4.65GGLAGSDD793 pKa = 3.37TVDD796 pKa = 3.52YY797 pKa = 10.94SAGAAVTVDD806 pKa = 4.31LFTGTGVGGNAQGDD820 pKa = 4.36TLVHH824 pKa = 5.63IQNVIGSSAGGNTFYY839 pKa = 11.43ASADD843 pKa = 3.41ANGFNGNSTSGNTVNYY859 pKa = 9.8SHH861 pKa = 6.99ATGAVIVDD869 pKa = 3.96LTNTRR874 pKa = 11.84DD875 pKa = 3.2NTLAQNFATGDD886 pKa = 3.58TFTNIQNVVGSVYY899 pKa = 10.95DD900 pKa = 4.0DD901 pKa = 5.29LIVATGQVNRR911 pKa = 11.84IDD913 pKa = 4.19GGLGNNTVSYY923 pKa = 9.29EE924 pKa = 4.01QALTAVKK931 pKa = 10.53VDD933 pKa = 3.91LATNQGSLGDD943 pKa = 4.08AAGDD947 pKa = 3.53TYY949 pKa = 11.81LNIQNAIGGAGNDD962 pKa = 3.26ILVGNASVNKK972 pKa = 9.69FDD974 pKa = 5.44GGLGSNTVSYY984 pKa = 10.07EE985 pKa = 3.81HH986 pKa = 6.6ATAAVKK992 pKa = 10.83VNLASGLGLSGDD1004 pKa = 3.96AAGDD1008 pKa = 3.64TYY1010 pKa = 12.0ANIQNAIGGAGDD1022 pKa = 4.5DD1023 pKa = 4.42IIVASAVANAIDD1035 pKa = 3.91GGGGSGNTVSYY1046 pKa = 9.8EE1047 pKa = 3.87NSSAGVKK1054 pKa = 10.04VDD1056 pKa = 4.08LFLGQGFNNDD1066 pKa = 3.62AAGDD1070 pKa = 3.93TYY1072 pKa = 11.69VRR1074 pKa = 11.84IQNAQGGNGDD1084 pKa = 3.67DD1085 pKa = 3.77TFVGNADD1092 pKa = 3.46VNNFEE1097 pKa = 5.0GGNGSNTVSYY1107 pKa = 9.22EE1108 pKa = 3.91HH1109 pKa = 7.01SSAAVKK1115 pKa = 10.47INLATGSGSLGDD1127 pKa = 3.84AAGDD1131 pKa = 3.17RR1132 pKa = 11.84YY1133 pKa = 11.56YY1134 pKa = 11.07NIQNAIGGLGNDD1146 pKa = 4.39TIVGSAATNAIDD1158 pKa = 4.53GGAGVNTVSYY1168 pKa = 9.78EE1169 pKa = 3.99NSTGAVKK1176 pKa = 10.85VNLATGQGLLGDD1188 pKa = 4.84ALNDD1192 pKa = 3.58TYY1194 pKa = 11.79VRR1196 pKa = 11.84IQNVTGGAGNDD1207 pKa = 3.43ILVGSADD1214 pKa = 3.51VNVIDD1219 pKa = 5.82GGLGNNTVSYY1229 pKa = 9.88EE1230 pKa = 4.15SSTGAVTIDD1239 pKa = 3.73LKK1241 pKa = 10.74QAQGLAGDD1249 pKa = 4.39AAGDD1253 pKa = 3.74TYY1255 pKa = 11.52FNIQNATGGAGDD1267 pKa = 4.22DD1268 pKa = 4.7LIVGSSAVNVIKK1280 pKa = 10.81GGAGVNTVSYY1290 pKa = 8.5ATSTAGVKK1298 pKa = 10.05VDD1300 pKa = 3.9LANNIGTGGDD1310 pKa = 3.67ANLDD1314 pKa = 3.61TYY1316 pKa = 11.69EE1317 pKa = 4.82NIQKK1321 pKa = 10.57VIGSALDD1328 pKa = 3.43DD1329 pKa = 3.74TFVGNGQLNEE1339 pKa = 4.09FWGGTGTNTVSYY1351 pKa = 9.0EE1352 pKa = 3.88ASTGAVTIDD1361 pKa = 3.97LGQQIGTAGDD1371 pKa = 3.48AAGDD1375 pKa = 4.23LYY1377 pKa = 11.31HH1378 pKa = 7.82DD1379 pKa = 4.25IQNATGGAGNDD1390 pKa = 4.21LIIGSAVANRR1400 pKa = 11.84IDD1402 pKa = 4.17GGAGNNTVSYY1412 pKa = 9.58EE1413 pKa = 3.96NSTAAVKK1420 pKa = 10.87VNLVSNQGSLGDD1432 pKa = 3.95AAGDD1436 pKa = 3.58TYY1438 pKa = 11.94VNIQNAIGGAGDD1450 pKa = 5.14DD1451 pKa = 3.39IFVGNGVANRR1461 pKa = 11.84FDD1463 pKa = 3.55GRR1465 pKa = 11.84AGSNTISYY1473 pKa = 8.73EE1474 pKa = 3.93SSGSVTVDD1482 pKa = 3.36LNLGTGTAGDD1492 pKa = 4.18ANGDD1496 pKa = 3.26IYY1498 pKa = 11.65LNIQNFLGGAADD1510 pKa = 3.85DD1511 pKa = 4.13TVVASSVANVIDD1523 pKa = 3.91GGGTSLHH1530 pKa = 6.0NRR1532 pKa = 11.84VSYY1535 pKa = 10.37AASTAAVTVDD1545 pKa = 3.95LNYY1548 pKa = 10.4TDD1550 pKa = 3.85GTGTSGGYY1558 pKa = 9.79AAGDD1562 pKa = 3.68KK1563 pKa = 10.72LSNIQDD1569 pKa = 3.93LTGSIYY1575 pKa = 10.82DD1576 pKa = 3.68DD1577 pKa = 3.67TFVASAAANRR1587 pKa = 11.84FDD1589 pKa = 4.11GGGTSSHH1596 pKa = 5.75NRR1598 pKa = 11.84VSYY1601 pKa = 10.08AASNAGVTVDD1611 pKa = 5.08LNYY1614 pKa = 10.45TDD1616 pKa = 3.85GTGTSGGYY1624 pKa = 9.78AAGDD1628 pKa = 3.58TLVNIQDD1635 pKa = 4.2LTGSAYY1641 pKa = 10.88NDD1643 pKa = 3.55LFVASAASNNFDD1655 pKa = 3.94GGAGSNTVSYY1665 pKa = 10.19AASTAAVTVNLSPSVGSGNGGYY1687 pKa = 10.36AAGDD1691 pKa = 3.63TFTNIQNVIGSAYY1704 pKa = 9.89ADD1706 pKa = 3.7TLIGYY1711 pKa = 7.51ATVGVSSVLTGGAGGDD1727 pKa = 3.7TLIGIAANRR1736 pKa = 11.84AYY1738 pKa = 7.34TTASYY1743 pKa = 10.35AGSSAGVTVDD1753 pKa = 4.62LNYY1756 pKa = 10.68SDD1758 pKa = 4.06GTGTSGGDD1766 pKa = 3.2ATGDD1770 pKa = 2.89RR1771 pKa = 11.84LSFINNLIGSSFNDD1785 pKa = 3.35TFVANAQANSFDD1797 pKa = 4.04GGAGTDD1803 pKa = 3.46TVSYY1807 pKa = 8.65ATSTSAVTVDD1817 pKa = 4.48LSVTDD1822 pKa = 4.24GSGTSGGYY1830 pKa = 9.56AAGDD1834 pKa = 2.94RR1835 pKa = 11.84FVNIEE1840 pKa = 3.98NLIGSAYY1847 pKa = 10.78NDD1849 pKa = 3.7VLKK1852 pKa = 10.91GAAGGNSVIVGGGGADD1868 pKa = 3.61NITGVGTGNYY1878 pKa = 9.62ASYY1881 pKa = 10.17VGSGAAVTIDD1891 pKa = 3.33LRR1893 pKa = 11.84TGVGTGGDD1901 pKa = 3.53AQGDD1905 pKa = 3.86QLNNIYY1911 pKa = 10.73NVIGSSFNDD1920 pKa = 3.38TFIASSQANVFVGGGGVDD1938 pKa = 3.37TVSYY1942 pKa = 10.08IGSTAGVTVDD1952 pKa = 4.69LSNTTGANTSGGYY1965 pKa = 9.82AAGDD1969 pKa = 3.71SFNGISNIVGSNFSDD1984 pKa = 3.23TFYY1987 pKa = 11.51ASAVANFFDD1996 pKa = 5.07GGSGTNTVNYY2006 pKa = 9.75ARR2008 pKa = 11.84STAGVTVDD2016 pKa = 4.4LFHH2019 pKa = 6.57NTGANGYY2026 pKa = 9.55AAGDD2030 pKa = 3.75TYY2032 pKa = 12.01ANIQNATGSAFADD2045 pKa = 3.72LFYY2048 pKa = 11.31ANSSGNAFVGGGGSDD2063 pKa = 3.29TVSYY2067 pKa = 10.82QYY2069 pKa = 10.82ATGSAITASLASGTGSGGDD2088 pKa = 3.51AAGDD2092 pKa = 3.76TYY2094 pKa = 11.63SGIFNLIGSASVNSTLTGDD2113 pKa = 3.88GNGNTLTALGTGTTNVLNAGNAASGQTDD2141 pKa = 4.46IINAQQGGMNTLYY2154 pKa = 10.72AGTGTSTFNVSAGGVNNGIVNASTGTDD2181 pKa = 3.68NLNHH2185 pKa = 7.09AYY2187 pKa = 10.13GAGSAGTTTLHH2198 pKa = 6.81FDD2200 pKa = 3.3NLAATLTLSKK2210 pKa = 10.37FSADD2214 pKa = 3.0ATGISVLDD2222 pKa = 3.76VSSGSNTNMLVRR2234 pKa = 11.84ATDD2237 pKa = 3.57VTSMGVNNVLTIKK2250 pKa = 8.17MTATEE2255 pKa = 4.78SIQIDD2260 pKa = 4.01TTDD2263 pKa = 2.83TAYY2266 pKa = 10.88SYY2268 pKa = 11.15LIFGNGDD2275 pKa = 3.55YY2276 pKa = 11.11VFYY2279 pKa = 10.84AAADD2283 pKa = 3.85TNHH2286 pKa = 5.4VNEE2289 pKa = 4.36VARR2292 pKa = 11.84IHH2294 pKa = 6.14IQLAAA2299 pKa = 3.95

Molecular weight: 227.72 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|J2VUT1|J2VUT1_9BURK Putative redox protein regulator of disulfide bond formation OS=Herbaspirillum sp. YR522 OX=1144342 GN=PMI40_00270 PE=4 SV=1
MM1 pKa = 7.65PWLLAVRR8 pKa = 11.84RR9 pKa = 11.84KK10 pKa = 9.79KK11 pKa = 9.93HH12 pKa = 5.92LRR14 pKa = 11.84LHH16 pKa = 5.01QHH18 pKa = 7.08LLPKK22 pKa = 10.3RR23 pKa = 11.84LPLPQHH29 pKa = 5.78QLPKK33 pKa = 10.47RR34 pKa = 11.84LPLPLLLLKK43 pKa = 10.46RR44 pKa = 11.84LPLLLLLLKK53 pKa = 10.42HH54 pKa = 6.44LLLLPPMQSQLTLHH68 pKa = 6.24LQSNRR73 pKa = 11.84KK74 pKa = 9.31IIFF77 pKa = 3.58

Molecular weight: 9.23 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4612

0

4612

1510888

12

5836

327.6

35.62

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.387 ± 0.048

0.838 ± 0.011

5.447 ± 0.032

4.948 ± 0.037

3.51 ± 0.024

8.184 ± 0.06

2.235 ± 0.018

4.922 ± 0.029

3.235 ± 0.034

10.752 ± 0.049

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.551 ± 0.019

2.951 ± 0.038

4.846 ± 0.034

4.496 ± 0.03

6.774 ± 0.045

5.731 ± 0.03

5.059 ± 0.04

7.45 ± 0.027

1.312 ± 0.015

2.372 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski