Erwinia phage pEp_SNUABM_08

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.24

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 79 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5J6DAD4|A0A5J6DAD4_9CAUD Uncharacterized protein OS=Erwinia phage pEp_SNUABM_08 OX=2593268 GN=pEpSNUABM08_20 PE=4 SV=1
MM1 pKa = 7.71SIGLILSVCMPLVSGGDD18 pKa = 3.51DD19 pKa = 3.49CDD21 pKa = 4.43SYY23 pKa = 11.62IVDD26 pKa = 4.28VYY28 pKa = 11.52DD29 pKa = 3.68SVIPCVVEE37 pKa = 4.01MEE39 pKa = 4.67KK40 pKa = 10.69NPQKK44 pKa = 11.03LDD46 pKa = 3.5DD47 pKa = 5.16RR48 pKa = 11.84YY49 pKa = 10.59LSCAAVDD56 pKa = 3.58HH57 pKa = 6.31TVLIDD62 pKa = 3.62TRR64 pKa = 11.84DD65 pKa = 3.37HH66 pKa = 5.86RR67 pKa = 11.84TAAQIIADD75 pKa = 4.69LNAEE79 pKa = 4.4LPYY82 pKa = 10.32MGATKK87 pKa = 10.51

Molecular weight: 9.5 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5J6DAA0|A0A5J6DAA0_9CAUD Putative exonuclease OS=Erwinia phage pEp_SNUABM_08 OX=2593268 GN=pEpSNUABM08_52 PE=4 SV=1
MM1 pKa = 7.52KK2 pKa = 10.07PLLTCLALILAFVNLAVATLATPVVFAWQIIAGAWRR38 pKa = 11.84GFRR41 pKa = 11.84NASYY45 pKa = 11.06IMRR48 pKa = 11.84KK49 pKa = 8.16WFVTIIKK56 pKa = 10.28NYY58 pKa = 10.73LEE60 pKa = 5.01GIKK63 pKa = 10.05QLL65 pKa = 3.77

Molecular weight: 7.37 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

79

0

79

19524

29

1375

247.1

27.53

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.849 ± 0.536

0.891 ± 0.124

6.874 ± 0.286

6.028 ± 0.265

3.795 ± 0.177

7.288 ± 0.298

1.67 ± 0.181

5.142 ± 0.138

5.286 ± 0.295

7.667 ± 0.232

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.17 ± 0.163

4.379 ± 0.242

4.548 ± 0.332

4.364 ± 0.31

5.798 ± 0.228

5.276 ± 0.179

6.346 ± 0.292

6.597 ± 0.252

1.788 ± 0.14

3.242 ± 0.149
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski