Desulfurivibrio alkaliphilus (strain DSM 19089 / UNIQEM U267 / AHT2)

Taxonomy: cellular organisms; Bacteria; Proteobacteria; delta/epsilon subdivisions; Deltaproteobacteria; Desulfobacterales; Desulfobulbaceae; Desulfurivibrio; Desulfurivibrio alkaliphilus

Average proteome isoelectric point is 6.53

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2595 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|D6Z4S8|D6Z4S8_DESAT Beta-lactamase domain-containing protein OS=Desulfurivibrio alkaliphilus (strain DSM 19089 / UNIQEM U267 / AHT2) OX=589865 GN=DaAHT2_1873 PE=4 SV=1
MM1 pKa = 7.18SQRR4 pKa = 11.84PLLQPVVFLAALVCLLAVTFWSAPPATAAEE34 pKa = 4.32EE35 pKa = 4.36VATVVALRR43 pKa = 11.84GTAVAVDD50 pKa = 3.19AAGAEE55 pKa = 3.82RR56 pKa = 11.84ALAIKK61 pKa = 10.48APIHH65 pKa = 5.65RR66 pKa = 11.84QDD68 pKa = 3.83TIRR71 pKa = 11.84TGPRR75 pKa = 11.84GRR77 pKa = 11.84LQLMFTDD84 pKa = 3.63NTIISLGVNAEE95 pKa = 4.16LEE97 pKa = 4.02IAEE100 pKa = 4.4YY101 pKa = 10.56QWDD104 pKa = 3.86QQEE107 pKa = 4.82GEE109 pKa = 4.22LNTRR113 pKa = 11.84VNQGAFRR120 pKa = 11.84VMGGSVARR128 pKa = 11.84TSPEE132 pKa = 3.64KK133 pKa = 10.28FTTDD137 pKa = 2.92TPAATIGIRR146 pKa = 11.84GSMYY150 pKa = 10.44AGRR153 pKa = 11.84VSDD156 pKa = 3.68GALAVVFQGGTGIIVSNPAGTVEE179 pKa = 3.81ITTPGMGTRR188 pKa = 11.84VASRR192 pKa = 11.84TAAPTPPTVFTQEE205 pKa = 4.06DD206 pKa = 3.9LGALEE211 pKa = 5.0DD212 pKa = 4.06EE213 pKa = 4.84EE214 pKa = 4.74VDD216 pKa = 3.51EE217 pKa = 4.6EE218 pKa = 4.2AAEE221 pKa = 4.18EE222 pKa = 4.15EE223 pKa = 4.56TVEE226 pKa = 4.7DD227 pKa = 3.8EE228 pKa = 4.73TPAEE232 pKa = 4.18EE233 pKa = 4.16PAEE236 pKa = 4.24EE237 pKa = 4.19EE238 pKa = 4.34WEE240 pKa = 4.13DD241 pKa = 3.53AALDD245 pKa = 4.03EE246 pKa = 5.0PEE248 pKa = 4.09EE249 pKa = 4.11AAAPVAAAPAPITDD263 pKa = 3.8TAVDD267 pKa = 3.64QATEE271 pKa = 4.24TTTDD275 pKa = 3.23AAQDD279 pKa = 3.25EE280 pKa = 4.78WEE282 pKa = 4.28QTVVEE287 pKa = 4.81ADD289 pKa = 4.16PEE291 pKa = 4.31PTDD294 pKa = 4.65PDD296 pKa = 3.72PTDD299 pKa = 4.37PEE301 pKa = 5.56PIDD304 pKa = 4.6PEE306 pKa = 4.05PTDD309 pKa = 3.66PSEE312 pKa = 3.88AVYY315 pKa = 10.33SGRR318 pKa = 11.84GIEE321 pKa = 4.44IIYY324 pKa = 10.58SSMQEE329 pKa = 4.01EE330 pKa = 4.81VAVEE334 pKa = 3.93HH335 pKa = 6.82LVLTDD340 pKa = 3.82KK341 pKa = 10.9PSEE344 pKa = 4.02LTEE347 pKa = 4.14IEE349 pKa = 4.45AGLLEE354 pKa = 4.39YY355 pKa = 10.97NEE357 pKa = 4.32QSDD360 pKa = 4.17FSDD363 pKa = 3.92GEE365 pKa = 4.34YY366 pKa = 10.53SAVWSYY372 pKa = 11.82TDD374 pKa = 3.27ATQVEE379 pKa = 4.87EE380 pKa = 4.48WEE382 pKa = 4.51EE383 pKa = 4.27GDD385 pKa = 5.54DD386 pKa = 5.09FDD388 pKa = 5.32FQFTFVMVDD397 pKa = 3.21DD398 pKa = 5.35RR399 pKa = 11.84GEE401 pKa = 3.81FWLYY405 pKa = 8.31EE406 pKa = 3.92QWSDD410 pKa = 4.08TEE412 pKa = 4.37TDD414 pKa = 3.56EE415 pKa = 4.35SWTYY419 pKa = 8.82SEE421 pKa = 5.73KK422 pKa = 10.09MFFYY426 pKa = 10.67GSEE429 pKa = 4.35TPVDD433 pKa = 4.11LMPTSGGAIYY443 pKa = 10.38HH444 pKa = 6.14GFFTGASDD452 pKa = 3.84DD453 pKa = 3.67VDD455 pKa = 4.19YY456 pKa = 11.19GWEE459 pKa = 3.96EE460 pKa = 3.9FFGDD464 pKa = 5.03FSLAVNWEE472 pKa = 3.95TGKK475 pKa = 10.41VVGYY479 pKa = 10.17FIDD482 pKa = 4.25DD483 pKa = 3.41EE484 pKa = 5.07HH485 pKa = 8.35YY486 pKa = 10.5GRR488 pKa = 11.84PIPVYY493 pKa = 9.71LTGEE497 pKa = 4.08VDD499 pKa = 3.83GSSLSSVSIFGLDD512 pKa = 3.21YY513 pKa = 11.29DD514 pKa = 5.02DD515 pKa = 5.39YY516 pKa = 11.87GDD518 pKa = 4.11GALYY522 pKa = 10.11DD523 pKa = 3.46AWITTGSGTFAQFYY537 pKa = 7.12GTYY540 pKa = 8.79YY541 pKa = 10.37QGLGLVAEE549 pKa = 4.85GQSGSLYY556 pKa = 10.66YY557 pKa = 10.77DD558 pKa = 4.2DD559 pKa = 5.85AQEE562 pKa = 4.15DD563 pKa = 4.08WSVVGGGFRR572 pKa = 11.84DD573 pKa = 3.7PVFTLPEE580 pKa = 3.86QAASSTWRR588 pKa = 11.84GFVTAMEE595 pKa = 4.45INLVEE600 pKa = 4.4EE601 pKa = 4.86PGTPPEE607 pKa = 4.3LFRR610 pKa = 11.84STSAEE615 pKa = 3.98YY616 pKa = 10.74FEE618 pKa = 5.33LQIDD622 pKa = 4.1RR623 pKa = 11.84QAGMFSGQIIEE634 pKa = 4.76AEE636 pKa = 4.36SATTTGLSIGGLEE649 pKa = 3.94VAGFYY654 pKa = 11.08VSDD657 pKa = 3.6NFLAGEE663 pKa = 4.56LSSASDD669 pKa = 3.45SDD671 pKa = 3.79LQGALGGSGEE681 pKa = 4.35YY682 pKa = 10.63YY683 pKa = 10.4FYY685 pKa = 11.22DD686 pKa = 3.97FEE688 pKa = 6.94DD689 pKa = 3.84EE690 pKa = 4.42ADD692 pKa = 3.64YY693 pKa = 10.7FQIGEE698 pKa = 4.25LAEE701 pKa = 4.26HH702 pKa = 6.19VSWGYY707 pKa = 9.68WGAAFTGQDD716 pKa = 3.46EE717 pKa = 4.55MPYY720 pKa = 9.93LISPAGSFWVAGEE733 pKa = 4.11LTPADD738 pKa = 4.09TVLGMIDD745 pKa = 3.73AEE747 pKa = 4.48VTGTYY752 pKa = 10.16EE753 pKa = 4.2GGAEE757 pKa = 4.38GILIFEE763 pKa = 4.48GTMADD768 pKa = 4.41LYY770 pKa = 11.35NGQTTLNINFGDD782 pKa = 3.78ANINGSITFSEE793 pKa = 4.38IAEE796 pKa = 4.13LTIDD800 pKa = 4.15GSSSLGPDD808 pKa = 3.56GFAADD813 pKa = 3.68VTSVEE818 pKa = 4.33FFGGEE823 pKa = 3.74PTLASGGISGAFFGPNAEE841 pKa = 4.13SVGGNFHH848 pKa = 7.25AEE850 pKa = 3.64GGGAQFLGIFGGDD863 pKa = 2.89RR864 pKa = 3.28

Molecular weight: 92.64 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|D6Z0V9|D6Z0V9_DESAT Putative membrane protein insertion efficiency factor OS=Desulfurivibrio alkaliphilus (strain DSM 19089 / UNIQEM U267 / AHT2) OX=589865 GN=DaAHT2_2555 PE=3 SV=1
MM1 pKa = 7.74SKK3 pKa = 8.95RR4 pKa = 11.84TYY6 pKa = 10.22QPSNTKK12 pKa = 8.42RR13 pKa = 11.84HH14 pKa = 5.05RR15 pKa = 11.84THH17 pKa = 6.71GFRR20 pKa = 11.84VRR22 pKa = 11.84MSTKK26 pKa = 10.09NGRR29 pKa = 11.84AVINRR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84AKK38 pKa = 9.58GRR40 pKa = 11.84KK41 pKa = 8.66RR42 pKa = 11.84LAVV45 pKa = 3.41

Molecular weight: 5.37 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2595

0

2595

903674

30

2439

348.2

38.45

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.575 ± 0.06

1.186 ± 0.025

5.138 ± 0.033

6.861 ± 0.046

3.674 ± 0.022

7.965 ± 0.043

2.269 ± 0.026

5.096 ± 0.035

3.684 ± 0.044

11.524 ± 0.066

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.271 ± 0.019

2.951 ± 0.033

5.239 ± 0.038

4.172 ± 0.032

7.255 ± 0.045

4.895 ± 0.032

4.696 ± 0.03

6.791 ± 0.04

1.142 ± 0.017

2.614 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski