Microbacterium sorbitolivorans

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micrococcales; Microbacteriaceae; Microbacterium

Average proteome isoelectric point is 5.92

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2725 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A367Y1N4|A0A367Y1N4_9MICO Cyclic pyranopterin monophosphate synthase OS=Microbacterium sorbitolivorans OX=1867410 GN=moaC PE=3 SV=1
MM1 pKa = 7.7PLALTHH7 pKa = 7.31RR8 pKa = 11.84ITVALVAVVCGVSLLGGCTSSVPHH32 pKa = 6.56LADD35 pKa = 3.51PTEE38 pKa = 4.26TAALTVSPSDD48 pKa = 3.46TLTPTATPEE57 pKa = 3.78QKK59 pKa = 8.63QTNDD63 pKa = 3.38AADD66 pKa = 4.5PDD68 pKa = 3.9TWLVTEE74 pKa = 4.48SAIGPIEE81 pKa = 4.1IGADD85 pKa = 3.4FDD87 pKa = 4.16STLTTIRR94 pKa = 11.84STGVGTLDD102 pKa = 4.11CEE104 pKa = 4.38GVAYY108 pKa = 10.16GFAADD113 pKa = 3.6NAYY116 pKa = 10.31DD117 pKa = 3.31ILMIGDD123 pKa = 3.92RR124 pKa = 11.84EE125 pKa = 4.12GDD127 pKa = 3.19SGAISEE133 pKa = 5.25VSIGWNSDD141 pKa = 2.94TMGVGPRR148 pKa = 11.84TAEE151 pKa = 3.83ALGLGSTKK159 pKa = 10.76DD160 pKa = 3.42QVLAAYY166 pKa = 8.87EE167 pKa = 4.07DD168 pKa = 4.1AVEE171 pKa = 4.34EE172 pKa = 4.24DD173 pKa = 3.93SQIVGHH179 pKa = 6.15TYY181 pKa = 8.94VTIPDD186 pKa = 3.55KK187 pKa = 11.48DD188 pKa = 3.94GVSKK192 pKa = 10.91LVFGYY197 pKa = 10.52RR198 pKa = 11.84DD199 pKa = 3.89GYY201 pKa = 11.1DD202 pKa = 3.24GAVSVSVITGEE213 pKa = 3.99EE214 pKa = 3.78PAYY217 pKa = 9.85EE218 pKa = 4.23PCAA221 pKa = 3.79

Molecular weight: 22.79 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A367XZP8|A0A367XZP8_9MICO Phosphoglycerate dehydrogenase OS=Microbacterium sorbitolivorans OX=1867410 GN=DTO57_11210 PE=4 SV=1
MM1 pKa = 7.4GSVIKK6 pKa = 10.38KK7 pKa = 8.42RR8 pKa = 11.84RR9 pKa = 11.84KK10 pKa = 9.06RR11 pKa = 11.84MSKK14 pKa = 9.76KK15 pKa = 9.54KK16 pKa = 9.79HH17 pKa = 5.67RR18 pKa = 11.84KK19 pKa = 8.31LLRR22 pKa = 11.84KK23 pKa = 7.78TRR25 pKa = 11.84HH26 pKa = 3.65QRR28 pKa = 11.84RR29 pKa = 11.84NKK31 pKa = 9.78KK32 pKa = 9.85

Molecular weight: 4.1 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2725

0

2725

909936

21

1908

333.9

35.85

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.203 ± 0.075

0.478 ± 0.01

6.39 ± 0.046

6.13 ± 0.043

3.241 ± 0.026

8.894 ± 0.041

1.94 ± 0.021

5.018 ± 0.036

2.226 ± 0.035

9.706 ± 0.049

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.923 ± 0.02

2.156 ± 0.025

5.081 ± 0.036

2.78 ± 0.024

7.024 ± 0.056

5.773 ± 0.032

5.958 ± 0.033

8.469 ± 0.043

1.539 ± 0.02

2.071 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski