Bacillus phage 019DV002

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.23

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 83 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5J6T581|A0A5J6T581_9CAUD Major tail protein OS=Bacillus phage 019DV002 OX=2601653 GN=24 PE=4 SV=1
MM1 pKa = 6.92EE2 pKa = 4.4TAICSYY8 pKa = 10.5CGEE11 pKa = 4.26EE12 pKa = 4.18KK13 pKa = 10.76EE14 pKa = 5.31IVASDD19 pKa = 3.61NQGHH23 pKa = 6.25KK24 pKa = 10.22FCYY27 pKa = 9.93DD28 pKa = 3.14CMYY31 pKa = 11.2GDD33 pKa = 5.13DD34 pKa = 4.7PNIPVCPEE42 pKa = 3.11GDD44 pKa = 3.65AYY46 pKa = 11.17CEE48 pKa = 4.04EE49 pKa = 4.1QRR51 pKa = 11.84NGYY54 pKa = 9.32IDD56 pKa = 3.76SKK58 pKa = 10.51FMNMCYY64 pKa = 9.95EE65 pKa = 3.88VDD67 pKa = 3.46QRR69 pKa = 11.84EE70 pKa = 4.18AANDD74 pKa = 3.82GTEE77 pKa = 4.09SEE79 pKa = 4.73GTGEE83 pKa = 4.11EE84 pKa = 4.1PAPAEE89 pKa = 4.35DD90 pKa = 4.15SEE92 pKa = 4.54EE93 pKa = 4.33GAA95 pKa = 4.49

Molecular weight: 10.49 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5J6T7Q3|A0A5J6T7Q3_9CAUD Plasmid replication-relaxation protein OS=Bacillus phage 019DV002 OX=2601653 GN=70 PE=4 SV=1
MM1 pKa = 7.36NKK3 pKa = 9.63LRR5 pKa = 11.84TSKK8 pKa = 10.32LWWLGCGFTIKK19 pKa = 10.33YY20 pKa = 9.92RR21 pKa = 11.84PEE23 pKa = 3.69RR24 pKa = 11.84DD25 pKa = 3.04EE26 pKa = 4.24FVIRR30 pKa = 11.84TGVFGRR36 pKa = 11.84LRR38 pKa = 11.84IDD40 pKa = 3.14RR41 pKa = 11.84HH42 pKa = 4.94SMRR45 pKa = 11.84RR46 pKa = 11.84VWTEE50 pKa = 3.28KK51 pKa = 10.22KK52 pKa = 9.57FLRR55 pKa = 11.84GRR57 pKa = 11.84RR58 pKa = 11.84LVIEE62 pKa = 4.51GVGKK66 pKa = 10.31RR67 pKa = 11.84YY68 pKa = 10.03RR69 pKa = 11.84FRR71 pKa = 11.84CIWGSHH77 pKa = 5.09SYY79 pKa = 9.96KK80 pKa = 10.44AKK82 pKa = 9.69QFIMEE87 pKa = 4.28RR88 pKa = 11.84ASLKK92 pKa = 10.14EE93 pKa = 4.01VNN95 pKa = 3.82

Molecular weight: 11.62 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

83

0

83

16050

32

1031

193.4

21.93

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.685 ± 0.312

0.916 ± 0.131

5.751 ± 0.229

8.336 ± 0.439

4.231 ± 0.151

6.741 ± 0.289

1.688 ± 0.156

6.561 ± 0.275

7.956 ± 0.385

8.075 ± 0.287

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.822 ± 0.152

4.891 ± 0.233

3.159 ± 0.161

3.713 ± 0.194

4.361 ± 0.227

6.312 ± 0.253

6.056 ± 0.375

6.131 ± 0.187

1.29 ± 0.112

4.324 ± 0.248
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski