Clostridium sp. KLE 1755

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae; Clostridium; unclassified Clostridium

Average proteome isoelectric point is 6.46

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6954 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|U2BET2|U2BET2_9CLOT Recombination protein RecR OS=Clostridium sp. KLE 1755 OX=1226325 GN=recR PE=3 SV=1
MM1 pKa = 7.54KK2 pKa = 10.22KK3 pKa = 8.92MAAAVVAMVTALALTACAGGFDD25 pKa = 3.48ASKK28 pKa = 10.67YY29 pKa = 8.01VQGVLNNIYY38 pKa = 10.5LGDD41 pKa = 3.68SAAYY45 pKa = 9.3MEE47 pKa = 4.92MVDD50 pKa = 3.72ITAEE54 pKa = 4.03EE55 pKa = 3.87AKK57 pKa = 10.54EE58 pKa = 3.88EE59 pKa = 4.22YY60 pKa = 9.63EE61 pKa = 4.1QGVEE65 pKa = 4.06VEE67 pKa = 5.36ADD69 pKa = 3.79FFLQYY74 pKa = 10.8YY75 pKa = 10.56GLDD78 pKa = 3.5TVSDD82 pKa = 3.69EE83 pKa = 4.55VYY85 pKa = 10.63QEE87 pKa = 3.89IVDD90 pKa = 4.38MYY92 pKa = 9.72HH93 pKa = 6.61QIYY96 pKa = 10.03SKK98 pKa = 11.14SKK100 pKa = 9.5FEE102 pKa = 4.04VKK104 pKa = 10.14EE105 pKa = 3.86AVKK108 pKa = 11.0NGDD111 pKa = 3.71DD112 pKa = 3.92FNVEE116 pKa = 4.34VVISPIDD123 pKa = 3.69VIVNSEE129 pKa = 4.16DD130 pKa = 3.92AISAAVDD137 pKa = 3.41EE138 pKa = 5.99FIANANADD146 pKa = 4.0DD147 pKa = 4.28YY148 pKa = 12.05ADD150 pKa = 3.74EE151 pKa = 4.14QAVNDD156 pKa = 4.36ALAKK160 pKa = 10.56LVVDD164 pKa = 4.84TINGNMANLGYY175 pKa = 8.34QTEE178 pKa = 3.97KK179 pKa = 11.0SIIVKK184 pKa = 9.03VEE186 pKa = 3.48KK187 pKa = 10.31DD188 pKa = 3.07AQGYY192 pKa = 9.45YY193 pKa = 10.46GISDD197 pKa = 4.0DD198 pKa = 5.79AISALDD204 pKa = 3.46QDD206 pKa = 4.67MIAYY210 pKa = 9.3

Molecular weight: 22.91 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|U2C7P5|U2C7P5_9CLOT Transcriptional regulator GntR family OS=Clostridium sp. KLE 1755 OX=1226325 GN=HMPREF1548_06823 PE=4 SV=1
MM1 pKa = 7.67KK2 pKa = 8.73MTFQPKK8 pKa = 8.64NRR10 pKa = 11.84QRR12 pKa = 11.84NKK14 pKa = 8.02VHH16 pKa = 6.59GFRR19 pKa = 11.84ARR21 pKa = 11.84MSTPGGRR28 pKa = 11.84KK29 pKa = 8.8VLAARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.92GRR39 pKa = 11.84KK40 pKa = 8.91RR41 pKa = 11.84LSAA44 pKa = 3.96

Molecular weight: 5.08 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6954

0

6954

2014820

35

3511

289.7

32.6

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.68 ± 0.031

1.69 ± 0.014

5.243 ± 0.024

7.582 ± 0.034

4.52 ± 0.021

7.432 ± 0.031

1.724 ± 0.014

6.792 ± 0.032

5.999 ± 0.024

9.444 ± 0.032

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.108 ± 0.015

4.077 ± 0.017

3.686 ± 0.017

3.314 ± 0.017

5.037 ± 0.029

5.797 ± 0.023

5.033 ± 0.021

6.345 ± 0.022

1.198 ± 0.014

4.299 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski