Paenibacillus rigui

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Paenibacillaceae; Paenibacillus

Average proteome isoelectric point is 6.43

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6159 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A229UKA0|A0A229UKA0_9BACL Aspartate ammonia-lyase OS=Paenibacillus rigui OX=554312 GN=aspA PE=3 SV=1
MM1 pKa = 7.07YY2 pKa = 10.54KK3 pKa = 10.39EE4 pKa = 4.27IADD7 pKa = 4.05EE8 pKa = 4.85DD9 pKa = 5.53LIDD12 pKa = 4.63CYY14 pKa = 11.7VNAVQMQLDD23 pKa = 3.93TDD25 pKa = 4.22FLGLLLYY32 pKa = 11.08AMEE35 pKa = 4.08QRR37 pKa = 11.84NLRR40 pKa = 11.84VSEE43 pKa = 3.9EE44 pKa = 3.93DD45 pKa = 4.97LYY47 pKa = 11.31FQYY50 pKa = 11.21KK51 pKa = 10.39LEE53 pKa = 4.11

Molecular weight: 6.4 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A229UP62|A0A229UP62_9BACL Cobalt ABC transporter ATP-binding protein OS=Paenibacillus rigui OX=554312 GN=CF651_16180 PE=4 SV=1
MM1 pKa = 7.9RR2 pKa = 11.84PTFKK6 pKa = 10.87PNVRR10 pKa = 11.84KK11 pKa = 9.84RR12 pKa = 11.84KK13 pKa = 8.89KK14 pKa = 8.64VHH16 pKa = 5.57GFRR19 pKa = 11.84KK20 pKa = 10.01RR21 pKa = 11.84MSTKK25 pKa = 10.18NGRR28 pKa = 11.84KK29 pKa = 8.87VLAARR34 pKa = 11.84RR35 pKa = 11.84LKK37 pKa = 10.2GRR39 pKa = 11.84KK40 pKa = 8.79VLSAA44 pKa = 4.05

Molecular weight: 5.19 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6159

0

6159

2066054

26

3348

335.5

37.35

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.393 ± 0.037

0.767 ± 0.009

5.014 ± 0.022

6.456 ± 0.037

4.056 ± 0.023

7.39 ± 0.035

2.203 ± 0.017

6.411 ± 0.029

5.394 ± 0.035

10.093 ± 0.04

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.792 ± 0.019

3.737 ± 0.028

4.187 ± 0.022

4.266 ± 0.023

4.936 ± 0.029

6.399 ± 0.03

5.551 ± 0.031

7.102 ± 0.025

1.34 ± 0.013

3.514 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski