Brevibacterium celere

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micrococcales; Brevibacteriaceae; Brevibacterium

Average proteome isoelectric point is 5.81

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3344 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A366IR78|A0A366IR78_9MICO Mak_N_cap domain-containing protein OS=Brevibacterium celere OX=225845 GN=DFO65_101339 PE=4 SV=1
MM1 pKa = 7.47TFNPDD6 pKa = 2.96ADD8 pKa = 3.29ISGNTTSRR16 pKa = 11.84RR17 pKa = 11.84GRR19 pKa = 11.84TIGIAGGGVGVVGLLVFLVGPLLGIDD45 pKa = 3.43VTGLVGDD52 pKa = 4.49GMLEE56 pKa = 4.34GGDD59 pKa = 3.87SGQSSGEE66 pKa = 4.09SSLLEE71 pKa = 4.51CDD73 pKa = 3.17TGEE76 pKa = 4.2DD77 pKa = 3.43ANRR80 pKa = 11.84SVDD83 pKa = 3.31CRR85 pKa = 11.84MAGAQVVLDD94 pKa = 4.93DD95 pKa = 3.89YY96 pKa = 9.12WTQHH100 pKa = 4.49VEE102 pKa = 4.42GYY104 pKa = 9.85RR105 pKa = 11.84PPQMTIVDD113 pKa = 4.27GQTSTGCGTASNAVGPFYY131 pKa = 11.0CPADD135 pKa = 3.28QGVYY139 pKa = 9.79IDD141 pKa = 4.41PAFFDD146 pKa = 3.63LMRR149 pKa = 11.84EE150 pKa = 3.95RR151 pKa = 11.84FGASAGEE158 pKa = 3.92LAQLYY163 pKa = 10.02IVGHH167 pKa = 5.52EE168 pKa = 4.22WGHH171 pKa = 6.42HH172 pKa = 4.54IQNITGTMDD181 pKa = 3.07EE182 pKa = 4.94HH183 pKa = 7.0PNNGTGPDD191 pKa = 3.67SNGVRR196 pKa = 11.84MEE198 pKa = 4.16LQADD202 pKa = 4.87CYY204 pKa = 10.76AGAWLGEE211 pKa = 4.38VTTLTDD217 pKa = 4.06DD218 pKa = 3.49NGVPYY223 pKa = 10.79LEE225 pKa = 5.4APTEE229 pKa = 4.25VQLSDD234 pKa = 3.43ALNAAFVVGDD244 pKa = 4.27DD245 pKa = 4.99HH246 pKa = 7.18IQSQSGFVNPEE257 pKa = 3.94SFTHH261 pKa = 5.78GTSEE265 pKa = 4.76QRR267 pKa = 11.84QKK269 pKa = 10.69WFTQGYY275 pKa = 5.64EE276 pKa = 4.08TGLGSCDD283 pKa = 3.11SFAVRR288 pKa = 11.84GGEE291 pKa = 3.9LL292 pKa = 3.2

Molecular weight: 30.81 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A366ILR5|A0A366ILR5_9MICO Nitrite reductase/ring-hydroxylating ferredoxin subunit OS=Brevibacterium celere OX=225845 GN=DFO65_102195 PE=4 SV=1
MM1 pKa = 6.73TARR4 pKa = 11.84RR5 pKa = 11.84SPALPLLWAGLATIVAGAAMSLQGRR30 pKa = 11.84ANGILGPVLGHH41 pKa = 6.78AVFAALLSFATGLLLVGGALLLSARR66 pKa = 11.84SRR68 pKa = 11.84AAAVRR73 pKa = 11.84LFSLVRR79 pKa = 11.84TGALPRR85 pKa = 11.84WMLLGGCSGALVVIAQATTVPVMGIAMFTMAFVSGQVTGGLCVDD129 pKa = 4.32ATHH132 pKa = 7.2LPPGGRR138 pKa = 11.84QRR140 pKa = 11.84LTFFRR145 pKa = 11.84ILGVLVVLAALTLGAWEE162 pKa = 4.2RR163 pKa = 11.84LVLGVPLWAPLLPFASGALTALQQACNGRR192 pKa = 11.84IRR194 pKa = 11.84AATGSAVVATAVNFAVGFLLLLALTGVLLASGVRR228 pKa = 11.84WTGFPGPGQWWILLGGVLGVVFIAFTSLTVRR259 pKa = 11.84RR260 pKa = 11.84LGVLLLTLFSLFGNLAGALLLDD282 pKa = 4.62LAVPLPGSLVSTTTVLSAALVLAGIAVTLMPAAGSLRR319 pKa = 11.84GRR321 pKa = 11.84GSRR324 pKa = 11.84TRR326 pKa = 11.84GPRR329 pKa = 11.84RR330 pKa = 11.84GPSRR334 pKa = 11.84RR335 pKa = 11.84RR336 pKa = 11.84RR337 pKa = 11.84GTSRR341 pKa = 11.84GSRR344 pKa = 11.84GRR346 pKa = 11.84RR347 pKa = 11.84SAAGARR353 pKa = 11.84TRR355 pKa = 11.84SRR357 pKa = 3.25

Molecular weight: 36.59 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3344

0

3344

1135316

29

2868

339.5

36.43

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.534 ± 0.06

0.653 ± 0.01

6.269 ± 0.034

6.292 ± 0.042

3.229 ± 0.026

8.934 ± 0.04

2.16 ± 0.022

4.842 ± 0.033

2.186 ± 0.035

9.952 ± 0.046

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.959 ± 0.015

1.982 ± 0.023

5.358 ± 0.031

2.603 ± 0.021

7.146 ± 0.042

6.205 ± 0.03

6.091 ± 0.027

8.383 ± 0.037

1.351 ± 0.014

1.872 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski