Microcystis aeruginosa (strain NIES-843)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Cyanobacteria/Melainabacteria group; Cyanobacteria; Oscillatoriophycideae; Chroococcales; Microcystaceae; Microcystis; Microcystis aeruginosa

Average proteome isoelectric point is 6.67

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5981 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|B0JI02|RL4_MICAN 50S ribosomal protein L4 OS=Microcystis aeruginosa (strain NIES-843) OX=449447 GN=rplD PE=3 SV=1
MM1 pKa = 7.21TIAVRR6 pKa = 11.84FLPDD10 pKa = 4.5DD11 pKa = 4.42IIIEE15 pKa = 4.32AEE17 pKa = 4.1TGEE20 pKa = 4.58LLLDD24 pKa = 3.6VAKK27 pKa = 10.58KK28 pKa = 10.59AGIDD32 pKa = 3.46IPTGCLMGSCHH43 pKa = 6.13ACEE46 pKa = 4.42VEE48 pKa = 5.45LDD50 pKa = 4.8DD51 pKa = 4.39GTQICSCITGIPGDD65 pKa = 3.79RR66 pKa = 11.84DD67 pKa = 3.58SLTVHH72 pKa = 7.11LYY74 pKa = 10.77SDD76 pKa = 4.43PLWW79 pKa = 5.13

Molecular weight: 8.48 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|B0JTF8|B0JTF8_MICAN Uncharacterized protein OS=Microcystis aeruginosa (strain NIES-843) OX=449447 GN=MAE_44390 PE=4 SV=1
MM1 pKa = 7.58RR2 pKa = 11.84LVKK5 pKa = 10.45RR6 pKa = 11.84MRR8 pKa = 11.84SLPRR12 pKa = 11.84TPVMLVKK19 pKa = 10.62NSKK22 pKa = 10.1PLMLVPFGRR31 pKa = 11.84LRR33 pKa = 11.84SARR36 pKa = 11.84VVPSMVRR43 pKa = 11.84LRR45 pKa = 11.84AVVATEE51 pKa = 4.33KK52 pKa = 10.67SASTRR57 pKa = 11.84VSIPAPPSTVSTPPPLVIRR76 pKa = 11.84SLLSPPLMLSSPSPPLRR93 pKa = 11.84VSLPALPIRR102 pKa = 11.84VSFKK106 pKa = 10.83LLPNRR111 pKa = 11.84LSLPLPPVRR120 pKa = 11.84LNLSIFLL127 pKa = 4.33

Molecular weight: 13.94 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5981

0

5981

1510946

29

4724

252.6

28.38

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.347 ± 0.041

1.07 ± 0.014

4.829 ± 0.021

6.531 ± 0.038

4.131 ± 0.023

6.494 ± 0.038

1.753 ± 0.015

7.225 ± 0.028

5.512 ± 0.033

11.281 ± 0.042

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.748 ± 0.015

4.526 ± 0.031

4.672 ± 0.026

5.06 ± 0.03

5.285 ± 0.031

6.439 ± 0.031

5.402 ± 0.038

5.976 ± 0.027

1.462 ± 0.017

3.257 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski