Amino acid dipepetide frequency for Operophtera brumata (winter moth)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	6.92AlaAla: 6.92 ± 0.053	1.531AlaCys: 1.531 ± 0.043	3.532AlaAsp: 3.532 ± 0.026	4.234AlaGlu: 4.234 ± 0.034	2.311AlaPhe: 2.311 ± 0.022	4.305AlaGly: 4.305 ± 0.042	1.827AlaHis: 1.827 ± 0.022	3.398AlaIle: 3.398 ± 0.03	3.697AlaLys: 3.697 ± 0.036	6.891AlaLeu: 6.891 ± 0.051	1.596AlaMet: 1.596 ± 0.02	2.589AlaAsn: 2.589 ± 0.022	3.971AlaPro: 3.971 ± 0.04	2.568AlaGln: 2.568 ± 0.024	4.42AlaArg: 4.42 ± 0.044	5.202AlaSer: 5.202 ± 0.038	3.927AlaThr: 3.927 ± 0.033	4.725AlaVal: 4.725 ± 0.035	0.751AlaTrp: 0.751 ± 0.015	1.901AlaTyr: 1.901 ± 0.02	0.005AlaXaa: 0.005 ± 0.001
Cys	1.509CysAla: 1.509 ± 0.026	0.509CysCys: 0.509 ± 0.014	1.244CysAsp: 1.244 ± 0.023	1.237CysGlu: 1.237 ± 0.024	0.743CysPhe: 0.743 ± 0.019	1.49CysGly: 1.49 ± 0.047	0.541CysHis: 0.541 ± 0.015	1.099CysIle: 1.099 ± 0.031	1.189CysLys: 1.189 ± 0.024	1.829CysLeu: 1.829 ± 0.028	0.45CysMet: 0.45 ± 0.011	0.946CysAsn: 0.946 ± 0.022	1.127CysPro: 1.127 ± 0.045	0.718CysGln: 0.718 ± 0.022	1.266CysArg: 1.266 ± 0.047	1.702CysSer: 1.702 ± 0.043	1.217CysThr: 1.217 ± 0.034	1.434CysVal: 1.434 ± 0.034	0.271CysTrp: 0.271 ± 0.008	0.641CysTyr: 0.641 ± 0.015	0.001CysXaa: 0.001 ± 0.0
Asp	3.511AspAla: 3.511 ± 0.029	1.061AspCys: 1.061 ± 0.024	3.716AspAsp: 3.716 ± 0.041	4.092AspGlu: 4.092 ± 0.038	2.108AspPhe: 2.108 ± 0.024	3.176AspGly: 3.176 ± 0.028	1.209AspHis: 1.209 ± 0.016	3.433AspIle: 3.433 ± 0.029	3.503AspLys: 3.503 ± 0.033	4.872AspLeu: 4.872 ± 0.039	1.334AspMet: 1.334 ± 0.016	2.646AspAsn: 2.646 ± 0.029	2.534AspPro: 2.534 ± 0.04	1.599AspGln: 1.599 ± 0.016	2.641AspArg: 2.641 ± 0.027	4.301AspSer: 4.301 ± 0.034	3.097AspThr: 3.097 ± 0.026	3.743AspVal: 3.743 ± 0.028	0.629AspTrp: 0.629 ± 0.012	1.854AspTyr: 1.854 ± 0.021	0.001AspXaa: 0.001 ± 0.0
Glu	4.216GluAla: 4.216 ± 0.039	1.317GluCys: 1.317 ± 0.049	3.793GluAsp: 3.793 ± 0.038	5.297GluGlu: 5.297 ± 0.057	2.091GluPhe: 2.091 ± 0.02	3.082GluGly: 3.082 ± 0.027	1.581GluHis: 1.581 ± 0.024	3.697GluIle: 3.697 ± 0.034	4.585GluLys: 4.585 ± 0.052	5.91GluLeu: 5.91 ± 0.043	1.537GluMet: 1.537 ± 0.019	3.411GluAsn: 3.411 ± 0.03	2.968GluPro: 2.968 ± 0.031	2.499GluGln: 2.499 ± 0.024	3.954GluArg: 3.954 ± 0.038	4.408GluSer: 4.408 ± 0.027	3.779GluThr: 3.779 ± 0.04	3.859GluVal: 3.859 ± 0.035	0.691GluTrp: 0.691 ± 0.013	2.01GluTyr: 2.01 ± 0.019	0.003GluXaa: 0.003 ± 0.001
Phe	2.184PheAla: 2.184 ± 0.024	0.772PheCys: 0.772 ± 0.013	2.062PheAsp: 2.062 ± 0.023	2.126PheGlu: 2.126 ± 0.022	1.367PhePhe: 1.367 ± 0.019	2.207PheGly: 2.207 ± 0.021	0.922PheHis: 0.922 ± 0.014	2.104PheIle: 2.104 ± 0.022	2.211PheLys: 2.211 ± 0.022	3.277PheLeu: 3.277 ± 0.031	0.869PheMet: 0.869 ± 0.013	1.794PheAsn: 1.794 ± 0.021	1.541PhePro: 1.541 ± 0.019	1.339PheGln: 1.339 ± 0.016	1.862PheArg: 1.862 ± 0.024	2.814PheSer: 2.814 ± 0.024	2.16PheThr: 2.16 ± 0.021	2.35PheVal: 2.35 ± 0.027	0.417PheTrp: 0.417 ± 0.01	1.36PheTyr: 1.36 ± 0.019	0.0PheXaa: 0.0 ± 0.0
Gly	4.308GlyAla: 4.308 ± 0.042	1.074GlyCys: 1.074 ± 0.02	3.045GlyAsp: 3.045 ± 0.029	3.217GlyGlu: 3.217 ± 0.032	2.087GlyPhe: 2.087 ± 0.025	4.104GlyGly: 4.104 ± 0.051	1.478GlyHis: 1.478 ± 0.021	2.835GlyIle: 2.835 ± 0.029	3.288GlyLys: 3.288 ± 0.028	4.619GlyLeu: 4.619 ± 0.037	1.221GlyMet: 1.221 ± 0.019	2.357GlyAsn: 2.357 ± 0.027	2.474GlyPro: 2.474 ± 0.041	1.967GlyGln: 1.967 ± 0.027	3.279GlyArg: 3.279 ± 0.033	4.338GlySer: 4.338 ± 0.04	3.114GlyThr: 3.114 ± 0.03	3.785GlyVal: 3.785 ± 0.03	0.73GlyTrp: 0.73 ± 0.014	1.929GlyTyr: 1.929 ± 0.027	0.003GlyXaa: 0.003 ± 0.001
His	1.799HisAla: 1.799 ± 0.025	0.62HisCys: 0.62 ± 0.013	1.245HisAsp: 1.245 ± 0.015	1.418HisGlu: 1.418 ± 0.019	0.979HisPhe: 0.979 ± 0.013	1.466HisGly: 1.466 ± 0.021	0.972HisHis: 0.972 ± 0.022	1.449HisIle: 1.449 ± 0.017	1.439HisLys: 1.439 ± 0.018	2.476HisLeu: 2.476 ± 0.027	0.692HisMet: 0.692 ± 0.018	1.222HisAsn: 1.222 ± 0.019	1.353HisPro: 1.353 ± 0.022	1.01HisGln: 1.01 ± 0.018	1.586HisArg: 1.586 ± 0.021	2.032HisSer: 2.032 ± 0.023	1.541HisThr: 1.541 ± 0.02	1.658HisVal: 1.658 ± 0.021	0.335HisTrp: 0.335 ± 0.009	0.945HisTyr: 0.945 ± 0.015	0.0HisXaa: 0.0 ± 0.0
Ile	3.483IleAla: 3.483 ± 0.027	1.188IleCys: 1.188 ± 0.028	3.153IleAsp: 3.153 ± 0.029	3.484IleGlu: 3.484 ± 0.032	2.023IlePhe: 2.023 ± 0.026	2.678IleGly: 2.678 ± 0.027	1.34IleHis: 1.34 ± 0.016	3.123IleIle: 3.123 ± 0.034	3.637IleLys: 3.637 ± 0.031	4.787IleLeu: 4.787 ± 0.04	1.177IleMet: 1.177 ± 0.016	2.754IleAsn: 2.754 ± 0.029	2.722IlePro: 2.722 ± 0.027	2.095IleGln: 2.095 ± 0.027	2.66IleArg: 2.66 ± 0.021	4.093IleSer: 4.093 ± 0.031	3.373IleThr: 3.373 ± 0.028	3.491IleVal: 3.491 ± 0.032	0.522IleTrp: 0.522 ± 0.015	1.704IleTyr: 1.704 ± 0.021	0.001IleXaa: 0.001 ± 0.0
Lys	3.611LysAla: 3.611 ± 0.034	1.308LysCys: 1.308 ± 0.031	3.395LysAsp: 3.395 ± 0.037	4.588LysGlu: 4.588 ± 0.052	2.138LysPhe: 2.138 ± 0.024	2.711LysGly: 2.711 ± 0.029	1.653LysHis: 1.653 ± 0.023	3.728LysIle: 3.728 ± 0.034	5.192LysLys: 5.192 ± 0.06	5.755LysLeu: 5.755 ± 0.041	1.535LysMet: 1.535 ± 0.015	3.211LysAsn: 3.211 ± 0.032	3.26LysPro: 3.26 ± 0.041	2.495LysGln: 2.495 ± 0.026	3.672LysArg: 3.672 ± 0.034	4.548LysSer: 4.548 ± 0.038	3.786LysThr: 3.786 ± 0.035	3.699LysVal: 3.699 ± 0.033	0.648LysTrp: 0.648 ± 0.012	2.201LysTyr: 2.201 ± 0.021	0.001LysXaa: 0.001 ± 0.001
Leu	6.67LeuAla: 6.67 ± 0.048	1.951LeuCys: 1.951 ± 0.021	4.865LeuAsp: 4.865 ± 0.037	5.783LeuGlu: 5.783 ± 0.046	3.165LeuPhe: 3.165 ± 0.036	4.675LeuGly: 4.675 ± 0.039	2.579LeuHis: 2.579 ± 0.029	4.258LeuIle: 4.258 ± 0.036	6.047LeuLys: 6.047 ± 0.049	8.773LeuLeu: 8.773 ± 0.066	2.047LeuMet: 2.047 ± 0.023	4.271LeuAsn: 4.271 ± 0.036	4.904LeuPro: 4.904 ± 0.035	4.195LeuGln: 4.195 ± 0.034	5.541LeuArg: 5.541 ± 0.045	7.005LeuSer: 7.005 ± 0.044	5.096LeuThr: 5.096 ± 0.04	5.496LeuVal: 5.496 ± 0.042	0.978LeuTrp: 0.978 ± 0.016	2.805LeuTyr: 2.805 ± 0.03	0.003LeuXaa: 0.003 ± 0.001
Met	1.703MetAla: 1.703 ± 0.018	0.487MetCys: 0.487 ± 0.012	1.283MetAsp: 1.283 ± 0.017	1.55MetGlu: 1.55 ± 0.015	0.944MetPhe: 0.944 ± 0.015	1.168MetGly: 1.168 ± 0.018	0.56MetHis: 0.56 ± 0.012	1.087MetIle: 1.087 ± 0.015	1.535MetLys: 1.535 ± 0.019	2.084MetLeu: 2.084 ± 0.024	0.648MetMet: 0.648 ± 0.014	1.078MetAsn: 1.078 ± 0.016	1.186MetPro: 1.186 ± 0.016	0.934MetGln: 0.934 ± 0.016	1.272MetArg: 1.272 ± 0.018	1.855MetSer: 1.855 ± 0.02	1.327MetThr: 1.327 ± 0.018	1.33MetVal: 1.33 ± 0.017	0.283MetTrp: 0.283 ± 0.008	0.74MetTyr: 0.74 ± 0.014	0.0MetXaa: 0.0 ± 0.0
Asn	2.776AsnAla: 2.776 ± 0.034	0.932AsnCys: 0.932 ± 0.018	2.479AsnAsp: 2.479 ± 0.025	3.084AsnGlu: 3.084 ± 0.033	1.783AsnPhe: 1.783 ± 0.019	2.662AsnGly: 2.662 ± 0.034	1.1AsnHis: 1.1 ± 0.021	3.224AsnIle: 3.224 ± 0.024	3.318AsnLys: 3.318 ± 0.034	4.183AsnLeu: 4.183 ± 0.038	1.208AsnMet: 1.208 ± 0.019	2.773AsnAsn: 2.773 ± 0.03	2.162AsnPro: 2.162 ± 0.037	1.677AsnGln: 1.677 ± 0.023	2.19AsnArg: 2.19 ± 0.024	3.522AsnSer: 3.522 ± 0.032	2.805AsnThr: 2.805 ± 0.03	3.127AsnVal: 3.127 ± 0.028	0.484AsnTrp: 0.484 ± 0.011	1.709AsnTyr: 1.709 ± 0.022	0.0AsnXaa: 0.0 ± 0.0
Pro	4.111ProAla: 4.111 ± 0.037	0.908ProCys: 0.908 ± 0.061	2.813ProAsp: 2.813 ± 0.024	3.574ProGlu: 3.574 ± 0.035	1.667ProPhe: 1.667 ± 0.024	2.977ProGly: 2.977 ± 0.046	1.387ProHis: 1.387 ± 0.02	2.46ProIle: 2.46 ± 0.026	3.035ProLys: 3.035 ± 0.03	4.332ProLeu: 4.332 ± 0.039	1.042ProMet: 1.042 ± 0.018	2.187ProAsn: 2.187 ± 0.031	4.314ProPro: 4.314 ± 0.055	2.155ProGln: 2.155 ± 0.031	3.074ProArg: 3.074 ± 0.037	4.273ProSer: 4.273 ± 0.048	3.208ProThr: 3.208 ± 0.042	3.526ProVal: 3.526 ± 0.036	0.512ProTrp: 0.512 ± 0.012	1.615ProTyr: 1.615 ± 0.023	0.003ProXaa: 0.003 ± 0.001
Gln	2.618GlnAla: 2.618 ± 0.028	0.841GlnCys: 0.841 ± 0.021	1.802GlnAsp: 1.802 ± 0.02	2.409GlnGlu: 2.409 ± 0.028	1.368GlnPhe: 1.368 ± 0.015	1.836GlnGly: 1.836 ± 0.025	1.192GlnHis: 1.192 ± 0.017	2.021GlnIle: 2.021 ± 0.02	2.329GlnLys: 2.329 ± 0.029	3.825GlnLeu: 3.825 ± 0.032	0.979GlnMet: 0.979 ± 0.016	2.0GlnAsn: 2.0 ± 0.024	2.106GlnPro: 2.106 ± 0.029	2.062GlnGln: 2.062 ± 0.04	2.452GlnArg: 2.452 ± 0.027	2.704GlnSer: 2.704 ± 0.025	2.135GlnThr: 2.135 ± 0.026	2.253GlnVal: 2.253 ± 0.027	0.443GlnTrp: 0.443 ± 0.009	1.342GlnTyr: 1.342 ± 0.018	0.001GlnXaa: 0.001 ± 0.0
Arg	4.486ArgAla: 4.486 ± 0.042	1.248ArgCys: 1.248 ± 0.03	3.235ArgAsp: 3.235 ± 0.033	3.434ArgGlu: 3.434 ± 0.031	1.855ArgPhe: 1.855 ± 0.023	3.291ArgGly: 3.291 ± 0.033	1.764ArgHis: 1.764 ± 0.021	2.66ArgIle: 2.66 ± 0.023	3.591ArgLys: 3.591 ± 0.036	5.245ArgLeu: 5.245 ± 0.039	1.256ArgMet: 1.256 ± 0.013	2.583ArgAsn: 2.583 ± 0.021	3.037ArgPro: 3.037 ± 0.038	2.28ArgGln: 2.28 ± 0.026	4.716ArgArg: 4.716 ± 0.052	4.223ArgSer: 4.223 ± 0.036	3.04ArgThr: 3.04 ± 0.028	3.484ArgVal: 3.484 ± 0.03	0.685ArgTrp: 0.685 ± 0.015	1.872ArgTyr: 1.872 ± 0.021	0.004ArgXaa: 0.004 ± 0.001
Ser	5.201SerAla: 5.201 ± 0.036	1.552SerCys: 1.552 ± 0.047	4.398SerAsp: 4.398 ± 0.034	4.778SerGlu: 4.778 ± 0.035	2.739SerPhe: 2.739 ± 0.026	4.554SerGly: 4.554 ± 0.039	1.909SerHis: 1.909 ± 0.022	3.925SerIle: 3.925 ± 0.034	4.574SerLys: 4.574 ± 0.037	6.822SerLeu: 6.822 ± 0.048	1.685SerMet: 1.685 ± 0.019	3.593SerAsn: 3.593 ± 0.034	4.505SerPro: 4.505 ± 0.061	2.856SerGln: 2.856 ± 0.026	4.178SerArg: 4.178 ± 0.035	7.175SerSer: 7.175 ± 0.06	4.739SerThr: 4.739 ± 0.043	4.89SerVal: 4.89 ± 0.034	0.856SerTrp: 0.856 ± 0.013	2.334SerTyr: 2.334 ± 0.027	0.003SerXaa: 0.003 ± 0.001
Thr	4.088ThrAla: 4.088 ± 0.031	1.313ThrCys: 1.313 ± 0.033	3.163ThrAsp: 3.163 ± 0.031	3.771ThrGlu: 3.771 ± 0.04	2.164ThrPhe: 2.164 ± 0.024	3.192ThrGly: 3.192 ± 0.032	1.468ThrHis: 1.468 ± 0.021	3.177ThrIle: 3.177 ± 0.026	3.385ThrLys: 3.385 ± 0.034	5.349ThrLeu: 5.349 ± 0.042	1.287ThrMet: 1.287 ± 0.017	2.647ThrAsn: 2.647 ± 0.026	3.628ThrPro: 3.628 ± 0.029	2.172ThrGln: 2.172 ± 0.025	2.985ThrArg: 2.985 ± 0.03	4.796ThrSer: 4.796 ± 0.04	3.962ThrThr: 3.962 ± 0.069	3.949ThrVal: 3.949 ± 0.039	0.638ThrTrp: 0.638 ± 0.012	1.798ThrTyr: 1.798 ± 0.027	0.002ThrXaa: 0.002 ± 0.001
Val	4.544ValAla: 4.544 ± 0.038	1.521ValCys: 1.521 ± 0.033	3.42ValAsp: 3.42 ± 0.026	3.984ValGlu: 3.984 ± 0.039	2.422ValPhe: 2.422 ± 0.026	3.162ValGly: 3.162 ± 0.029	1.599ValHis: 1.599 ± 0.022	3.429ValIle: 3.429 ± 0.029	3.857ValLys: 3.857 ± 0.033	5.94ValLeu: 5.94 ± 0.043	1.421ValMet: 1.421 ± 0.019	2.883ValAsn: 2.883 ± 0.031	3.484ValPro: 3.484 ± 0.035	2.437ValGln: 2.437 ± 0.028	3.575ValArg: 3.575 ± 0.029	4.992ValSer: 4.992 ± 0.036	4.063ValThr: 4.063 ± 0.036	4.326ValVal: 4.326 ± 0.035	0.732ValTrp: 0.732 ± 0.017	2.009ValTyr: 2.009 ± 0.022	0.001ValXaa: 0.001 ± 0.0
Trp	0.682TrpAla: 0.682 ± 0.014	0.239TrpCys: 0.239 ± 0.007	0.631TrpAsp: 0.631 ± 0.015	0.639TrpGlu: 0.639 ± 0.011	0.425TrpPhe: 0.425 ± 0.01	0.604TrpGly: 0.604 ± 0.012	0.285TrpHis: 0.285 ± 0.008	0.579TrpIle: 0.579 ± 0.011	0.698TrpLys: 0.698 ± 0.012	1.153TrpLeu: 1.153 ± 0.018	0.296TrpMet: 0.296 ± 0.009	0.53TrpAsn: 0.53 ± 0.013	0.459TrpPro: 0.459 ± 0.011	0.45TrpGln: 0.45 ± 0.01	0.842TrpArg: 0.842 ± 0.015	0.846TrpSer: 0.846 ± 0.015	0.658TrpThr: 0.658 ± 0.013	0.648TrpVal: 0.648 ± 0.014	0.194TrpTrp: 0.194 ± 0.006	0.358TrpTyr: 0.358 ± 0.01	0.0TrpXaa: 0.0 ± 0.0
Tyr	1.877TyrAla: 1.877 ± 0.019	0.79TyrCys: 0.79 ± 0.014	1.784TyrAsp: 1.784 ± 0.022	1.986TyrGlu: 1.986 ± 0.023	1.361TyrPhe: 1.361 ± 0.021	1.909TyrGly: 1.909 ± 0.022	0.867TyrHis: 0.867 ± 0.015	1.801TyrIle: 1.801 ± 0.021	2.02TyrLys: 2.02 ± 0.025	2.981TyrLeu: 2.981 ± 0.03	0.774TyrMet: 0.774 ± 0.013	1.724TyrAsn: 1.724 ± 0.022	1.433TyrPro: 1.433 ± 0.024	1.209TyrGln: 1.209 ± 0.017	1.822TyrArg: 1.822 ± 0.021	2.437TyrSer: 2.437 ± 0.026	1.908TyrThr: 1.908 ± 0.023	2.029TyrVal: 2.029 ± 0.024	0.418TyrTrp: 0.418 ± 0.011	1.303TyrTyr: 1.303 ± 0.021	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.004XaaAla: 0.004 ± 0.001	0.002XaaCys: 0.002 ± 0.001	0.001XaaAsp: 0.001 ± 0.0	0.001XaaGlu: 0.001 ± 0.0	0.001XaaPhe: 0.001 ± 0.0	0.001XaaGly: 0.001 ± 0.001	0.001XaaHis: 0.001 ± 0.0	0.002XaaIle: 0.002 ± 0.001	0.001XaaLys: 0.001 ± 0.0	0.004XaaLeu: 0.004 ± 0.001	0.001XaaMet: 0.001 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.004XaaPro: 0.004 ± 0.001	0.001XaaGln: 0.001 ± 0.0	0.002XaaArg: 0.002 ± 0.001	0.002XaaSer: 0.002 ± 0.001	0.002XaaThr: 0.002 ± 0.001	0.002XaaVal: 0.002 ± 0.001	0.0XaaTrp: 0.0 ± 0.0	0.001XaaTyr: 0.001 ± 0.0	0.017XaaXaa: 0.017 ± 0.006

Statistics based on 16814 proteins (6022392 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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