Gramella salexigens

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Flavobacteriia; Flavobacteriales; Flavobacteriaceae; Gramella

Average proteome isoelectric point is 6.26

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2591 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A1L3J5G0|A0A1L3J5G0_9FLAO Mechanosensitive ion channel protein OS=Gramella salexigens OX=1913577 GN=LPB144_08150 PE=3 SV=1
MM1 pKa = 8.1DD2 pKa = 4.13PTRR5 pKa = 11.84DD6 pKa = 3.22VDD8 pKa = 4.09RR9 pKa = 11.84DD10 pKa = 3.5GSPWFIDD17 pKa = 3.93CDD19 pKa = 4.8DD20 pKa = 4.47NNADD24 pKa = 5.34LNDD27 pKa = 3.88KK28 pKa = 11.42DD29 pKa = 4.59EE30 pKa = 6.1DD31 pKa = 3.67NDD33 pKa = 4.48NYY35 pKa = 10.12STCQGDD41 pKa = 5.13PDD43 pKa = 4.18DD44 pKa = 4.72QDD46 pKa = 4.07PNVYY50 pKa = 9.75PGSALICINQTVTAAQTSFICEE72 pKa = 4.11GEE74 pKa = 4.21TTINLGGSQTNVSYY88 pKa = 10.59YY89 pKa = 10.76LRR91 pKa = 11.84NNADD95 pKa = 3.03NSIIDD100 pKa = 3.81GPIAGTGSPISFNTGTISSNTTFNVYY126 pKa = 10.3SEE128 pKa = 4.74AGAKK132 pKa = 9.73GALDD136 pKa = 3.57FDD138 pKa = 5.5GIDD141 pKa = 3.87DD142 pKa = 4.25QVILDD147 pKa = 4.1GFDD150 pKa = 3.53LAGEE154 pKa = 4.15ANVTIEE160 pKa = 3.87AWVNPTDD167 pKa = 3.24VTGAHH172 pKa = 6.86KK173 pKa = 10.9GIISKK178 pKa = 9.45TNGDD182 pKa = 3.41GQFIFRR188 pKa = 11.84LEE190 pKa = 4.17NGHH193 pKa = 6.51PRR195 pKa = 11.84SVFYY199 pKa = 10.62TSTGDD204 pKa = 3.53LGSLSTQALTPGQWYY219 pKa = 9.43HH220 pKa = 6.0VVTTFDD226 pKa = 3.55GDD228 pKa = 3.3EE229 pKa = 3.86SRR231 pKa = 11.84IYY233 pKa = 11.03VNGVDD238 pKa = 3.89VTGAQYY244 pKa = 11.33NYY246 pKa = 10.2AASGGLKK253 pKa = 9.67TYY255 pKa = 7.47TASPAIIGRR264 pKa = 11.84ANPQEE269 pKa = 4.13FFDD272 pKa = 4.07GKK274 pKa = 10.04IGEE277 pKa = 4.11VRR279 pKa = 11.84VWNEE283 pKa = 3.47LKK285 pKa = 10.08TPAEE289 pKa = 3.83ITANMSSSYY298 pKa = 10.5TGTEE302 pKa = 3.69TGLVALYY309 pKa = 10.65KK310 pKa = 9.65MKK312 pKa = 10.08EE313 pKa = 4.13GKK315 pKa = 10.46GITVADD321 pKa = 3.73STGSFDD327 pKa = 3.69GTMEE331 pKa = 4.1NMDD334 pKa = 4.95PATDD338 pKa = 3.38WLTYY342 pKa = 10.92DD343 pKa = 4.18PDD345 pKa = 3.95CTLEE349 pKa = 4.15MTDD352 pKa = 4.15KK353 pKa = 10.7PSVTIAPIEE362 pKa = 4.27DD363 pKa = 3.44QVVTVAEE370 pKa = 4.4STLCPSNTGTTVNLEE385 pKa = 4.02TSQPDD390 pKa = 2.87ITYY393 pKa = 10.55YY394 pKa = 10.75LRR396 pKa = 11.84NDD398 pKa = 3.85ADD400 pKa = 4.21DD401 pKa = 5.1SIIDD405 pKa = 3.86GPVEE409 pKa = 4.03GGGAISFDD417 pKa = 3.54TGSITTTTNYY427 pKa = 10.0NVHH430 pKa = 6.78AVPTNGDD437 pKa = 3.9TSCSTQMSQTVAVTIEE453 pKa = 4.82DD454 pKa = 3.52NTAPTVIARR463 pKa = 11.84DD464 pKa = 3.28ITVQLDD470 pKa = 3.52EE471 pKa = 5.15NGQATITADD480 pKa = 3.31QVNNGSTDD488 pKa = 2.81NCTAIGNLTLSLDD501 pKa = 3.56KK502 pKa = 10.97STFSCADD509 pKa = 2.84ISSGGEE515 pKa = 3.87CGEE518 pKa = 4.01VSLLFNSSGIATGDD532 pKa = 3.09NNANVEE538 pKa = 4.26EE539 pKa = 4.84LGMNQLTMEE548 pKa = 4.48AWVKK552 pKa = 10.35PSSNVVNSVIRR563 pKa = 11.84KK564 pKa = 9.52AGDD567 pKa = 3.25YY568 pKa = 10.97DD569 pKa = 4.4LYY571 pKa = 10.69IYY573 pKa = 10.38DD574 pKa = 4.18GQLKK578 pKa = 10.76AEE580 pKa = 4.19VWYY583 pKa = 10.31KK584 pKa = 10.88AGSDD588 pKa = 2.79ASMYY592 pKa = 10.63QFTGPAITYY601 pKa = 7.31NAWSHH606 pKa = 5.05VAFVYY611 pKa = 10.85DD612 pKa = 3.49NTNPGVGYY620 pKa = 9.23FVVNGNTYY628 pKa = 9.68PSNGNAHH635 pKa = 6.31QIGSNGNLGIGSSTVYY651 pKa = 10.16GQSFNGLIDD660 pKa = 3.61EE661 pKa = 4.2VRR663 pKa = 11.84IWSVARR669 pKa = 11.84TAQNIQDD676 pKa = 4.18NLSGCITGSEE686 pKa = 4.34LGLEE690 pKa = 4.46AYY692 pKa = 10.53YY693 pKa = 10.41KK694 pKa = 9.38VQEE697 pKa = 4.38GSGSVLKK704 pKa = 10.87DD705 pKa = 3.68FSDD708 pKa = 3.61NGNDD712 pKa = 3.63LTIQGATWQQEE723 pKa = 4.12GGTTAGVPVTLTVGDD738 pKa = 4.06ANGNQSTATANVAVVDD754 pKa = 5.14IIAPIIRR761 pKa = 11.84TYY763 pKa = 11.44EE764 pKa = 4.7DD765 pKa = 3.13ILVEE769 pKa = 4.28LDD771 pKa = 3.57EE772 pKa = 5.15NGQASIRR779 pKa = 11.84GDD781 pKa = 3.5YY782 pKa = 10.89SVGPVAGKK790 pKa = 10.41LEE792 pKa = 4.29PQNSLSTTTPVDD804 pKa = 3.48CDD806 pKa = 3.63CPEE809 pKa = 4.44GYY811 pKa = 10.26VAVGYY816 pKa = 9.9EE817 pKa = 4.46GASGWILDD825 pKa = 4.74DD826 pKa = 3.97FRR828 pKa = 11.84LVCKK832 pKa = 10.17EE833 pKa = 3.67VLADD837 pKa = 3.84GGLGTEE843 pKa = 4.38TVEE846 pKa = 4.41TCFSGSRR853 pKa = 11.84TEE855 pKa = 4.07VTRR858 pKa = 11.84SDD860 pKa = 3.45MLTGNEE866 pKa = 3.96VLVGFEE872 pKa = 4.12VQDD875 pKa = 4.35GDD877 pKa = 4.12FQHH880 pKa = 6.18QLSSRR885 pKa = 11.84THH887 pKa = 6.02VGVKK891 pKa = 10.56GFGKK895 pKa = 10.57SLDD898 pKa = 3.48QVVAGNQNNIDD909 pKa = 3.65NTALAGIIGSGTGTTSLATTTNFAPAGHH937 pKa = 7.39AIVGMSVNQTSGYY950 pKa = 8.97SSSVSFKK957 pKa = 10.15YY958 pKa = 10.79APISSLLSIDD968 pKa = 4.08NGSTDD973 pKa = 3.0NCGIASITADD983 pKa = 3.43KK984 pKa = 11.49YY985 pKa = 11.56SFTCEE990 pKa = 3.9NIGEE994 pKa = 4.37NIVTITAMDD1003 pKa = 3.63VNGNEE1008 pKa = 4.01ASATAVVTVEE1018 pKa = 5.57DD1019 pKa = 3.68NTAPQAIVRR1028 pKa = 11.84NITVEE1033 pKa = 3.83LDD1035 pKa = 3.43ANGMASITPEE1045 pKa = 3.89MVDD1048 pKa = 3.62NGSSDD1053 pKa = 3.44NCATTEE1059 pKa = 3.86DD1060 pKa = 4.02LQLILDD1066 pKa = 4.0KK1067 pKa = 11.25SIFGCEE1073 pKa = 3.82DD1074 pKa = 3.32VVDD1077 pKa = 4.55KK1078 pKa = 11.33GVTPNLSALNFDD1090 pKa = 3.48GDD1092 pKa = 4.68DD1093 pKa = 3.69YY1094 pKa = 12.07VSIPDD1099 pKa = 3.69SPEE1102 pKa = 3.73LQLQGDD1108 pKa = 4.06MTIEE1112 pKa = 3.46AWFKK1116 pKa = 10.53VDD1118 pKa = 4.42EE1119 pKa = 4.56FTGDD1123 pKa = 2.95WVRR1126 pKa = 11.84VVGKK1130 pKa = 10.1GAGPEE1135 pKa = 4.41GPVGPRR1141 pKa = 11.84NYY1143 pKa = 10.58GLWYY1147 pKa = 9.82FPNGTWLFQQYY1158 pKa = 9.63GQGVQVAFNRR1168 pKa = 11.84PIDD1171 pKa = 3.57IGEE1174 pKa = 4.35WYY1176 pKa = 10.62HH1177 pKa = 5.28MAAVKK1182 pKa = 9.43TGNVAKK1188 pKa = 10.5LYY1190 pKa = 10.55INGEE1194 pKa = 4.41VVASNTGGTNPITSNDD1210 pKa = 3.82PLTIGYY1216 pKa = 10.28AGFHH1220 pKa = 6.66DD1221 pKa = 4.0HH1222 pKa = 7.5HH1223 pKa = 8.08IGQIDD1228 pKa = 4.13EE1229 pKa = 4.05VRR1231 pKa = 11.84LWNIARR1237 pKa = 11.84TDD1239 pKa = 3.67EE1240 pKa = 4.91EE1241 pKa = 4.33ILNDD1245 pKa = 3.43FTKK1248 pKa = 10.44TINPEE1253 pKa = 3.84TSGLLAYY1260 pKa = 11.03YY1261 pKa = 10.82NMEE1264 pKa = 4.23EE1265 pKa = 4.16ASGDD1269 pKa = 3.5ILNDD1273 pKa = 3.12QTVNGFNGDD1282 pKa = 3.46LQGFSQNEE1290 pKa = 3.72AWTGSSRR1297 pKa = 11.84RR1298 pKa = 11.84LGPVSTMLTVTDD1310 pKa = 3.99ASGNSSSSTAVVTVVDD1326 pKa = 5.05NIAATVVTQNITVALDD1342 pKa = 3.54EE1343 pKa = 5.2NGTATITPEE1352 pKa = 4.22MIDD1355 pKa = 3.82GGSSDD1360 pKa = 3.51NCEE1363 pKa = 3.93IASLEE1368 pKa = 4.02LDD1370 pKa = 3.54VTEE1373 pKa = 5.0FTCEE1377 pKa = 3.74NVGEE1381 pKa = 4.25NTVTLTAVDD1390 pKa = 3.93VNDD1393 pKa = 4.09NEE1395 pKa = 4.42SSATAVVTVEE1405 pKa = 4.14DD1406 pKa = 4.56NIPAEE1411 pKa = 4.2VLTQNITVQLDD1422 pKa = 3.6DD1423 pKa = 4.38MGMASITPEE1432 pKa = 4.12MIDD1435 pKa = 3.16NGSNDD1440 pKa = 3.21VCGIASMSLDD1450 pKa = 3.0ITDD1453 pKa = 4.58FTCEE1457 pKa = 3.85NVGEE1461 pKa = 4.14NMVTLTAVDD1470 pKa = 3.86VNDD1473 pKa = 4.09NEE1475 pKa = 4.38ASATATVIVEE1485 pKa = 4.21DD1486 pKa = 4.66NIAATVVTQNITVALDD1502 pKa = 3.54EE1503 pKa = 5.2NGTATITPEE1512 pKa = 4.17MIDD1515 pKa = 3.21NGSNDD1520 pKa = 3.16VCGIASLSLDD1530 pKa = 3.08ITDD1533 pKa = 4.57FTCEE1537 pKa = 3.84NVGEE1541 pKa = 4.25NTVTLTAVDD1550 pKa = 3.95VNDD1553 pKa = 4.09NEE1555 pKa = 4.38ASATATVIVEE1565 pKa = 4.15DD1566 pKa = 4.53KK1567 pKa = 10.68IAAEE1571 pKa = 4.1VLTQNITVQLDD1582 pKa = 3.59EE1583 pKa = 4.93MGMASITPEE1592 pKa = 4.21MIDD1595 pKa = 3.25NDD1597 pKa = 4.33SNDD1600 pKa = 3.24VCGIASLSLDD1610 pKa = 3.08ITDD1613 pKa = 4.57FTCEE1617 pKa = 3.84NVGEE1621 pKa = 4.25NTVTLTAVDD1630 pKa = 3.95VNDD1633 pKa = 4.09NEE1635 pKa = 4.38ASATATVIVEE1645 pKa = 4.15DD1646 pKa = 4.53KK1647 pKa = 10.68IAAEE1651 pKa = 4.1VLTQNITVQLDD1662 pKa = 3.76EE1663 pKa = 6.11LGMASITPEE1672 pKa = 4.12MIDD1675 pKa = 3.37NGSNDD1680 pKa = 3.24ACGIASMSLDD1690 pKa = 3.0ITDD1693 pKa = 4.58FTCEE1697 pKa = 3.84NVGEE1701 pKa = 4.25NTVTLTAVDD1710 pKa = 3.95VNDD1713 pKa = 4.09NEE1715 pKa = 4.38ASATATVIVEE1725 pKa = 4.15DD1726 pKa = 4.53KK1727 pKa = 10.68IAAEE1731 pKa = 4.1VLTQNITVQLDD1742 pKa = 3.6DD1743 pKa = 4.38MGMASITPEE1752 pKa = 4.02MINNGSNDD1760 pKa = 3.2VCGIASMSLDD1770 pKa = 3.0ITDD1773 pKa = 4.58FTCEE1777 pKa = 3.84NVGEE1781 pKa = 4.27NTVTLTVVDD1790 pKa = 4.24VNDD1793 pKa = 3.89NEE1795 pKa = 4.37ASATATVIVEE1805 pKa = 4.15DD1806 pKa = 4.53KK1807 pKa = 10.68IAAEE1811 pKa = 4.1VLTQNITVQLDD1822 pKa = 3.59EE1823 pKa = 4.93MGMASITPEE1832 pKa = 4.12MIDD1835 pKa = 3.37NGSNDD1840 pKa = 3.24ACGIASMSLDD1850 pKa = 3.0ITDD1853 pKa = 4.58FTCEE1857 pKa = 3.85NVGEE1861 pKa = 4.36NIVTLTAMDD1870 pKa = 3.84VNDD1873 pKa = 4.17NEE1875 pKa = 4.47ASATVIVTVEE1885 pKa = 3.97DD1886 pKa = 4.05KK1887 pKa = 10.84IAAEE1891 pKa = 4.1VLTQNITVQLDD1902 pKa = 3.59EE1903 pKa = 4.93MGMASITPEE1912 pKa = 4.12MIDD1915 pKa = 3.37NGSNDD1920 pKa = 2.95ACGIVSMSLDD1930 pKa = 3.15ITDD1933 pKa = 4.58FTCEE1937 pKa = 3.85NVGEE1941 pKa = 4.36NIVTLTAMDD1950 pKa = 3.84VNDD1953 pKa = 4.13NEE1955 pKa = 4.37ASATAIVTVEE1965 pKa = 4.06DD1966 pKa = 4.18KK1967 pKa = 10.84IAAEE1971 pKa = 4.47VITQNITVQLDD1982 pKa = 3.59EE1983 pKa = 4.93MGMASITPEE1992 pKa = 4.12MIDD1995 pKa = 3.37NGSNDD2000 pKa = 3.24ACGIASMSLDD2010 pKa = 3.69KK2011 pKa = 11.4SDD2013 pKa = 4.16FTCDD2017 pKa = 3.34HH2018 pKa = 6.62IGEE2021 pKa = 4.34NTVTLTAIDD2030 pKa = 3.21IHH2032 pKa = 8.19GNEE2035 pKa = 4.25SSATAIVSVEE2045 pKa = 4.09DD2046 pKa = 4.08VIAPTPVMDD2055 pKa = 3.88QLEE2058 pKa = 4.84PIYY2061 pKa = 10.64AEE2063 pKa = 4.52CIVEE2067 pKa = 4.21RR2068 pKa = 11.84TDD2070 pKa = 3.55VPTPLATDD2078 pKa = 3.2NCDD2081 pKa = 3.15IEE2083 pKa = 5.31ISATTDD2089 pKa = 3.1LVFPVTRR2096 pKa = 11.84QGSTMIRR2103 pKa = 11.84WKK2105 pKa = 11.03FEE2107 pKa = 4.03DD2108 pKa = 3.34SNANVTYY2115 pKa = 9.6QQQKK2119 pKa = 10.46IIIDD2123 pKa = 4.96DD2124 pKa = 3.44ITAPVPDD2131 pKa = 3.65IAEE2134 pKa = 4.38LEE2136 pKa = 4.42TVEE2139 pKa = 4.42VEE2141 pKa = 4.44CGVTAISAPTATDD2154 pKa = 2.93NCAGSVTGTTTDD2166 pKa = 3.45PLSYY2170 pKa = 10.45EE2171 pKa = 3.92EE2172 pKa = 4.07QGDD2175 pKa = 4.2YY2176 pKa = 11.0EE2177 pKa = 4.35ILWTFDD2183 pKa = 3.57DD2184 pKa = 4.83GNGNSTTQIQQVIVKK2199 pKa = 9.71DD2200 pKa = 3.47QTPPVALAKK2209 pKa = 10.52DD2210 pKa = 3.36ITVVLDD2216 pKa = 3.64PNDD2219 pKa = 3.39NVRR2222 pKa = 11.84INPEE2226 pKa = 4.49DD2227 pKa = 4.1IDD2229 pKa = 4.28DD2230 pKa = 4.25GSYY2233 pKa = 11.07DD2234 pKa = 3.65EE2235 pKa = 5.3CSEE2238 pKa = 4.08ISLSLDD2244 pKa = 2.86RR2245 pKa = 11.84DD2246 pKa = 4.01YY2247 pKa = 10.87FTSNGSYY2254 pKa = 10.23EE2255 pKa = 4.37VVLTVTDD2262 pKa = 3.83ALGNSSEE2269 pKa = 3.84ASARR2273 pKa = 11.84VIVVTNHH2280 pKa = 6.75PEE2282 pKa = 3.76TSNVHH2287 pKa = 4.77VVPTILKK2294 pKa = 7.92QSSMAKK2300 pKa = 9.59VIVPFRR2306 pKa = 11.84SRR2308 pKa = 11.84IIEE2311 pKa = 4.14VQVLEE2316 pKa = 4.45TEE2318 pKa = 4.22TNKK2321 pKa = 10.62YY2322 pKa = 10.31KK2323 pKa = 10.62IIRR2326 pKa = 11.84GNKK2329 pKa = 9.57LNEE2332 pKa = 3.89QQIDD2336 pKa = 3.33IAPFKK2341 pKa = 10.18GTLLVRR2347 pKa = 11.84IIDD2350 pKa = 3.47QDD2352 pKa = 3.65GRR2354 pKa = 11.84VHH2356 pKa = 6.86LKK2358 pKa = 10.68KK2359 pKa = 10.9LIALL2363 pKa = 4.36

Molecular weight:
250.43 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A1L3J3X5|A0A1L3J3X5_9FLAO Uncharacterized protein OS=Gramella salexigens OX=1913577 GN=LPB144_05140 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.59RR3 pKa = 11.84TFQPSKK9 pKa = 10.15RR10 pKa = 11.84KK11 pKa = 9.6RR12 pKa = 11.84KK13 pKa = 8.29NKK15 pKa = 9.34HH16 pKa = 4.03GFRR19 pKa = 11.84EE20 pKa = 4.2RR21 pKa = 11.84MASANGRR28 pKa = 11.84KK29 pKa = 9.04VLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.09GRR39 pKa = 11.84KK40 pKa = 7.97KK41 pKa = 10.73LSVSSEE47 pKa = 3.76NRR49 pKa = 11.84HH50 pKa = 4.47KK51 pKa = 10.5HH52 pKa = 4.49

Molecular weight:
6.23 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2591

0

2591

899170

44

5280

347.0

39.17

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.404 ± 0.049

0.694 ± 0.016

5.945 ± 0.063

7.408 ± 0.056

5.116 ± 0.044

6.619 ± 0.055

1.732 ± 0.027

7.72 ± 0.047

7.639 ± 0.077

9.274 ± 0.08

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.253 ± 0.031

5.806 ± 0.05

3.505 ± 0.031

3.207 ± 0.027

3.804 ± 0.047

6.655 ± 0.041

5.205 ± 0.1

6.027 ± 0.046

1.023 ± 0.018

3.965 ± 0.038

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski