Rubritalea squalenifaciens DSM 18772

Taxonomy: cellular organisms; Bacteria; PVC group; Verrucomicrobia; Verrucomicrobiae; Verrucomicrobiales; Rubritaleaceae; Rubritalea; Rubritalea squalenifaciens

Average proteome isoelectric point is 6.25

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3805 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A1M6ASZ7|A0A1M6ASZ7_9BACT N5-carboxyaminoimidazole ribonucleotide mutase OS=Rubritalea squalenifaciens DSM 18772 OX=1123071 GN=purE PE=3 SV=1
MM1 pKa = 7.27TPLIAFAQTLPDD13 pKa = 3.96SLVLNLDD20 pKa = 3.27TDD22 pKa = 4.5GNQTAEE28 pKa = 4.24TTVNLTKK35 pKa = 10.52RR36 pKa = 11.84SVRR39 pKa = 11.84APGVAYY45 pKa = 10.66YY46 pKa = 10.48RR47 pKa = 11.84FNGNSQTYY55 pKa = 8.14TLSPNDD61 pKa = 3.24IPQVRR66 pKa = 11.84TYY68 pKa = 10.41RR69 pKa = 11.84GTVAGDD75 pKa = 3.43PNVIVCATILPDD87 pKa = 3.6GTIKK91 pKa = 10.86ADD93 pKa = 3.91AFDD96 pKa = 3.87HH97 pKa = 6.19LKK99 pKa = 10.52GRR101 pKa = 11.84GYY103 pKa = 9.14VWQVSGYY110 pKa = 10.32DD111 pKa = 3.36VSASLNQGGAATANPTRR128 pKa = 11.84PEE130 pKa = 4.06GGMGEE135 pKa = 4.36LPMGVDD141 pKa = 3.16INYY144 pKa = 9.28HH145 pKa = 5.35RR146 pKa = 11.84FVNEE150 pKa = 3.92GASDD154 pKa = 4.28PDD156 pKa = 3.87LAWALTEE163 pKa = 4.46HH164 pKa = 6.54NFNIYY169 pKa = 10.65DD170 pKa = 3.72LMMGRR175 pKa = 11.84DD176 pKa = 3.22TGVAVSIATLVTRR189 pKa = 11.84EE190 pKa = 3.82NQEE193 pKa = 4.14YY194 pKa = 9.79YY195 pKa = 11.07VPTGSGNHH203 pKa = 5.73LNIIEE208 pKa = 4.75AEE210 pKa = 3.94WSDD213 pKa = 3.24AAKK216 pKa = 10.22PLRR219 pKa = 11.84NAAWEE224 pKa = 4.11QIFSYY229 pKa = 8.52KK230 pKa = 10.02TSVGLFGTGGYY241 pKa = 9.69AWQNRR246 pKa = 11.84IGKK249 pKa = 9.08NEE251 pKa = 4.23TINYY255 pKa = 7.07GQGAIGTQTLYY266 pKa = 10.95HH267 pKa = 6.81EE268 pKa = 5.02SGGHH272 pKa = 5.2NWNAHH277 pKa = 3.63HH278 pKa = 6.96WGYY281 pKa = 11.25GRR283 pKa = 11.84DD284 pKa = 3.5AMGSNRR290 pKa = 11.84PNHH293 pKa = 5.98GPYY296 pKa = 9.33NVEE299 pKa = 3.79RR300 pKa = 11.84VRR302 pKa = 11.84EE303 pKa = 3.93KK304 pKa = 10.88RR305 pKa = 11.84DD306 pKa = 3.13IKK308 pKa = 10.85IADD311 pKa = 3.74GTLLQPNGDD320 pKa = 4.26YY321 pKa = 10.53PSPAHH326 pKa = 6.78PYY328 pKa = 9.91SHH330 pKa = 7.57LDD332 pKa = 3.35LATTNVNQAVTISPLVNDD350 pKa = 3.89TDD352 pKa = 4.35FNGDD356 pKa = 3.75SISIVSFTATTANGGTVVQSGNDD379 pKa = 3.9LIYY382 pKa = 10.05TPPAGYY388 pKa = 9.71VGKK391 pKa = 10.22DD392 pKa = 3.05IIAYY396 pKa = 8.51TIEE399 pKa = 4.65DD400 pKa = 3.5NSGAGDD406 pKa = 3.81GGANLQAQDD415 pKa = 4.49LIFIEE420 pKa = 4.64VVNNGLTLKK429 pKa = 10.63FDD431 pKa = 4.41FNDD434 pKa = 3.06VSGASVSNSTGLGHH448 pKa = 6.62SAVLQSSNIEE458 pKa = 4.21SQLTTGPDD466 pKa = 3.49GYY468 pKa = 11.52ALVVPSGGLLVDD480 pKa = 4.37DD481 pKa = 4.87TNILPIPYY489 pKa = 8.86TGSTNNFYY497 pKa = 10.48PFDD500 pKa = 4.68SDD502 pKa = 4.91QMSQGNFFDD511 pKa = 5.7PMDD514 pKa = 4.67KK515 pKa = 10.69DD516 pKa = 3.68YY517 pKa = 11.42AICLWIKK524 pKa = 9.88MDD526 pKa = 5.08DD527 pKa = 4.15VITKK531 pKa = 10.46LDD533 pKa = 3.44ILDD536 pKa = 3.78KK537 pKa = 11.29YY538 pKa = 10.03KK539 pKa = 10.86AQEE542 pKa = 3.94RR543 pKa = 11.84STGFHH548 pKa = 6.4LFVEE552 pKa = 4.37NGVLKK557 pKa = 10.54ADD559 pKa = 3.83CGEE562 pKa = 4.01IEE564 pKa = 4.62GIYY567 pKa = 10.28NKK569 pKa = 10.36RR570 pKa = 11.84SITNGGAVTAGQWHH584 pKa = 6.29HH585 pKa = 6.12VALVFDD591 pKa = 4.38RR592 pKa = 11.84TADD595 pKa = 3.35QMKK598 pKa = 10.1IFLDD602 pKa = 4.73GAQTAQTALAAGSFIFHH619 pKa = 6.13GRR621 pKa = 11.84EE622 pKa = 3.43PLMIGSGSGGTMAVDD637 pKa = 3.96DD638 pKa = 4.2VRR640 pKa = 11.84LYY642 pKa = 9.08TQNLSSSDD650 pKa = 3.19LTGIMGEE657 pKa = 3.96ASNAVDD663 pKa = 4.54EE664 pKa = 4.64LALNNSLQTWWALDD678 pKa = 3.81DD679 pKa = 4.08NAGATASDD687 pKa = 3.37SSGNANAGALSGGAAWTTTAVDD709 pKa = 3.29GGGASFDD716 pKa = 3.95GVDD719 pKa = 5.02DD720 pKa = 5.29KK721 pKa = 11.64IKK723 pKa = 11.05LDD725 pKa = 3.86LAQSNVGAFTVAMWAKK741 pKa = 10.42SGADD745 pKa = 3.47GQGVYY750 pKa = 10.76SSVFSNRR757 pKa = 11.84TPNTGGTFQIDD768 pKa = 3.52MGNGFQYY775 pKa = 10.44RR776 pKa = 11.84GSQTSSFGAAPLDD789 pKa = 3.72QWVHH793 pKa = 5.7LAVVSDD799 pKa = 3.78GTDD802 pKa = 3.09TILYY806 pKa = 9.46YY807 pKa = 10.72NGEE810 pKa = 4.04IAKK813 pKa = 10.03VLSGVNDD820 pKa = 4.09DD821 pKa = 4.84LFNALVLGANRR832 pKa = 11.84NEE834 pKa = 3.86GDD836 pKa = 3.82FFQGEE841 pKa = 4.19IDD843 pKa = 3.8DD844 pKa = 3.85FCYY847 pKa = 9.74FDD849 pKa = 4.21RR850 pKa = 11.84ALNPSEE856 pKa = 3.6IQYY859 pKa = 10.44IKK861 pKa = 10.92SSLLKK866 pKa = 10.4NVAPSASDD874 pKa = 3.1DD875 pKa = 3.57TFAVVEE881 pKa = 4.11NASAGTSLGVVNASDD896 pKa = 4.18PNSGDD901 pKa = 3.72VVSHH905 pKa = 7.3AITGGNAAGLFAITAATGEE924 pKa = 4.31ITTTAPLDD932 pKa = 3.64YY933 pKa = 8.82EE934 pKa = 4.53TASSYY939 pKa = 11.54VLTVTVSDD947 pKa = 4.83DD948 pKa = 3.57GGLSDD953 pKa = 4.08TATITVNVTDD963 pKa = 4.03VANDD967 pKa = 3.69DD968 pKa = 3.93SDD970 pKa = 5.71ADD972 pKa = 3.82GLTDD976 pKa = 3.17EE977 pKa = 4.85WEE979 pKa = 4.17VANFGSVAATTGSADD994 pKa = 3.47SDD996 pKa = 3.65GDD998 pKa = 4.08GYY1000 pKa = 9.95TNAQEE1005 pKa = 4.19EE1006 pKa = 4.52ASATDD1011 pKa = 4.05PNNAASNPTTQVLAHH1026 pKa = 6.61WSLNEE1031 pKa = 3.79GAGLVATDD1039 pKa = 4.15SSVNGYY1045 pKa = 10.17DD1046 pKa = 3.52GQLTGGTAWTSSAVDD1061 pKa = 3.19AGGASFDD1068 pKa = 4.19GVDD1071 pKa = 4.66DD1072 pKa = 5.44RR1073 pKa = 11.84IDD1075 pKa = 3.49YY1076 pKa = 11.27ALTEE1080 pKa = 4.53TNLGAYY1086 pKa = 7.22TVAMWVKK1093 pKa = 10.38NGASGQSIYY1102 pKa = 11.38SSVFSNHH1109 pKa = 5.64TPNTSNTFQIDD1120 pKa = 3.18MGNGFNYY1127 pKa = 10.4RR1128 pKa = 11.84GTQTASFGAAPVGTWVHH1145 pKa = 6.12LAVVSDD1151 pKa = 3.87GTDD1154 pKa = 2.67TRR1156 pKa = 11.84LYY1158 pKa = 9.65YY1159 pKa = 10.81NGVLSQTLAGVNDD1172 pKa = 4.47DD1173 pKa = 4.83LFDD1176 pKa = 4.86TITLGTNRR1184 pKa = 11.84NEE1186 pKa = 4.11GNFFQGEE1193 pKa = 3.93VDD1195 pKa = 3.33EE1196 pKa = 5.65LYY1198 pKa = 11.07VYY1200 pKa = 10.68DD1201 pKa = 4.9AALNDD1206 pKa = 3.73EE1207 pKa = 5.71DD1208 pKa = 4.65ILALATPPAPNQPPVATNGSGSVAEE1233 pKa = 4.17NASVGTVVTTVVASDD1248 pKa = 4.36PDD1250 pKa = 3.76TGDD1253 pKa = 3.36TLSYY1257 pKa = 10.72SITAGNTGGAFAINSGTGEE1276 pKa = 4.13VTVAAGLDD1284 pKa = 3.79FEE1286 pKa = 6.33SISQYY1291 pKa = 10.74ILTVEE1296 pKa = 4.19VSDD1299 pKa = 5.48GSLTDD1304 pKa = 3.52TATVTIDD1311 pKa = 3.24VTNVNEE1317 pKa = 4.67APVANNGSGSVSEE1330 pKa = 4.47GASIGATVSTVSSSDD1345 pKa = 3.46PDD1347 pKa = 3.77TGDD1350 pKa = 3.14SATYY1354 pKa = 9.56TITAGNTGGAFVINSTSGAITTATGLDD1381 pKa = 3.96YY1382 pKa = 8.91EE1383 pKa = 4.72TTALYY1388 pKa = 10.95SLTVQVTDD1396 pKa = 4.22GGGLTDD1402 pKa = 3.64TATVTVNVTDD1412 pKa = 3.75VANDD1416 pKa = 3.69DD1417 pKa = 3.93SDD1419 pKa = 5.71ADD1421 pKa = 3.82GLTDD1425 pKa = 3.19EE1426 pKa = 5.01WEE1428 pKa = 4.13VSNFGSVAAQDD1439 pKa = 3.94GSGDD1443 pKa = 3.91ADD1445 pKa = 3.36SDD1447 pKa = 4.36GYY1449 pKa = 9.84TNAEE1453 pKa = 3.94EE1454 pKa = 4.32FAAGTDD1460 pKa = 3.6PSDD1463 pKa = 4.29PLSAPAPPDD1472 pKa = 3.71LVWTGAAGDD1481 pKa = 3.54GDD1483 pKa = 4.53FFNEE1487 pKa = 4.38ANWDD1491 pKa = 3.73SDD1493 pKa = 3.44ALTAGTQVPSADD1505 pKa = 3.36SVNVNQPLVASSLTVDD1521 pKa = 3.22SANFIGIGTVIPDD1534 pKa = 3.98GARR1537 pKa = 11.84IDD1539 pKa = 3.72ITNTTLTTSGSNGFNTTSSTLSEE1562 pKa = 4.32FNIGTGADD1570 pKa = 3.26VSFQFIANTTVSLAGDD1586 pKa = 3.43AVLEE1590 pKa = 4.16FRR1592 pKa = 11.84GGGNPVNGSTIDD1604 pKa = 3.69LAADD1608 pKa = 4.01FAGQVRR1614 pKa = 11.84FTSEE1618 pKa = 3.74TVASVLSEE1626 pKa = 4.14HH1627 pKa = 6.25MSKK1630 pKa = 8.48ITVAGQPAVNGVNAYY1645 pKa = 9.76VVDD1648 pKa = 4.14VNGVTVVSLEE1658 pKa = 4.15APNNAPVANDD1668 pKa = 3.24AAFTVAEE1675 pKa = 4.17NAGASATVGTVSVSDD1690 pKa = 4.29PDD1692 pKa = 5.53AGDD1695 pKa = 2.88SWTYY1699 pKa = 11.28AITAGNGGGEE1709 pKa = 4.05FAINSTTGEE1718 pKa = 4.07ITTTTSLDD1726 pKa = 3.53YY1727 pKa = 10.7EE1728 pKa = 4.66GASSYY1733 pKa = 11.51VLTVTVTDD1741 pKa = 3.81AGGLSDD1747 pKa = 3.74VATITVDD1754 pKa = 3.2VLNINEE1760 pKa = 4.16APVANSASGTVSEE1773 pKa = 4.79DD1774 pKa = 3.15VAVGVTVATVTSSDD1788 pKa = 3.4PDD1790 pKa = 3.79ANDD1793 pKa = 2.86TRR1795 pKa = 11.84TYY1797 pKa = 11.01AITAGNDD1804 pKa = 2.72GRR1806 pKa = 11.84FVINASTGEE1815 pKa = 4.03ITTVSALDD1823 pKa = 3.72YY1824 pKa = 7.19EE1825 pKa = 4.82TTNSYY1830 pKa = 11.33SLTVTVTDD1838 pKa = 4.16AGGLTATAMVAITVTDD1854 pKa = 3.8VVFEE1858 pKa = 5.34DD1859 pKa = 4.32YY1860 pKa = 11.13DD1861 pKa = 4.67SDD1863 pKa = 4.57SLDD1866 pKa = 4.49DD1867 pKa = 3.49NWEE1870 pKa = 3.76IANFGDD1876 pKa = 4.18TNTSDD1881 pKa = 3.39GTGDD1885 pKa = 3.6ADD1887 pKa = 5.38ADD1889 pKa = 3.82GLTDD1893 pKa = 3.78GEE1895 pKa = 4.57EE1896 pKa = 4.22YY1897 pKa = 10.9LAGSDD1902 pKa = 4.24PNSSDD1907 pKa = 3.15GDD1909 pKa = 3.43GDD1911 pKa = 3.89GFHH1914 pKa = 7.62DD1915 pKa = 3.71VLEE1918 pKa = 4.83INVGTDD1924 pKa = 3.03PMDD1927 pKa = 3.66ILDD1930 pKa = 4.86FPASEE1935 pKa = 4.21YY1936 pKa = 11.12DD1937 pKa = 5.19GLVLWWNLDD1946 pKa = 3.33EE1947 pKa = 4.66SAGASTVLDD1956 pKa = 3.45DD1957 pKa = 4.79TGNKK1961 pKa = 8.94MSGAVNGATLNGSEE1975 pKa = 4.52GSFDD1979 pKa = 3.8GSNDD1983 pKa = 4.39SIDD1986 pKa = 4.26LPLASGG1992 pKa = 3.65

Molecular weight:
205.83 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A1M6M0H0|A0A1M6M0H0_9BACT Thiol-disulfide interchange protein contains DsbC and DsbD domains OS=Rubritalea squalenifaciens DSM 18772 OX=1123071 GN=SAMN02745181_2559 PE=4 SV=1
MM1 pKa = 6.89STAHH5 pKa = 6.24EE6 pKa = 4.9KK7 pKa = 10.06IVHH10 pKa = 5.81AALRR14 pKa = 11.84DD15 pKa = 3.62ILEE18 pKa = 3.91QALRR22 pKa = 11.84RR23 pKa = 11.84LRR25 pKa = 11.84VVGGNDD31 pKa = 3.23LASKK35 pKa = 8.59VTVVWNSRR43 pKa = 11.84MRR45 pKa = 11.84STAGKK50 pKa = 9.59AHH52 pKa = 6.14WPEE55 pKa = 4.04AKK57 pKa = 10.38VEE59 pKa = 4.33LNPKK63 pKa = 10.13LIGISLAEE71 pKa = 4.19VKK73 pKa = 9.4RR74 pKa = 11.84TLLHH78 pKa = 6.27EE79 pKa = 4.42LAHH82 pKa = 6.23LLAYY86 pKa = 9.82HH87 pKa = 5.1RR88 pKa = 11.84HH89 pKa = 4.9KK90 pKa = 10.54RR91 pKa = 11.84RR92 pKa = 11.84RR93 pKa = 11.84IQPHH97 pKa = 4.21GRR99 pKa = 11.84EE100 pKa = 4.06WQQACADD107 pKa = 3.98LGIPGEE113 pKa = 4.52SVTHH117 pKa = 6.1NLALPSSRR125 pKa = 11.84QRR127 pKa = 11.84RR128 pKa = 11.84KK129 pKa = 8.05WRR131 pKa = 11.84YY132 pKa = 8.03VCPNCLQSIEE142 pKa = 4.38RR143 pKa = 11.84VRR145 pKa = 11.84KK146 pKa = 8.43MSASSACYY154 pKa = 9.68HH155 pKa = 6.76CCQNYY160 pKa = 10.52NGGKK164 pKa = 7.36YY165 pKa = 10.01HH166 pKa = 7.05SRR168 pKa = 11.84FRR170 pKa = 11.84LKK172 pKa = 10.74AEE174 pKa = 4.9RR175 pKa = 11.84IAA177 pKa = 4.61

Molecular weight:
20.31 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3805

0

3805

1291566

39

5242

339.4

37.62

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.554 ± 0.043

1.056 ± 0.014

5.861 ± 0.036

6.738 ± 0.048

3.876 ± 0.024

7.635 ± 0.052

2.281 ± 0.022

5.643 ± 0.028

5.661 ± 0.053

9.716 ± 0.049

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.368 ± 0.022

3.766 ± 0.029

4.463 ± 0.027

3.638 ± 0.026

5.031 ± 0.038

6.83 ± 0.049

5.675 ± 0.047

6.614 ± 0.044

1.462 ± 0.016

3.132 ± 0.027

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski