Faecalibacterium phage FP_Epona

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Eponavirus; Faecalibacterium virus Epona

Average proteome isoelectric point is 6.22

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 76 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2K9V342|A0A2K9V342_9CAUD Uncharacterized protein OS=Faecalibacterium phage FP_Epona OX=2070182 PE=4 SV=1
MM1 pKa = 6.95KK2 pKa = 9.59TYY4 pKa = 10.94TEE6 pKa = 4.16MTDD9 pKa = 3.46QEE11 pKa = 4.77LLNAYY16 pKa = 9.59LEE18 pKa = 4.61SGTYY22 pKa = 9.87DD23 pKa = 3.51PEE25 pKa = 4.11MCAEE29 pKa = 3.99MCKK32 pKa = 9.34RR33 pKa = 11.84TGLDD37 pKa = 3.75EE38 pKa = 4.0EE39 pKa = 4.6WAAADD44 pKa = 3.75ADD46 pKa = 4.08DD47 pKa = 5.32FEE49 pKa = 5.87GVVNTAAAKK58 pKa = 10.21LDD60 pKa = 4.1PNHH63 pKa = 6.56EE64 pKa = 4.73SII66 pKa = 5.43

Molecular weight: 7.35 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2K9V2Y9|A0A2K9V2Y9_9CAUD Uncharacterized protein OS=Faecalibacterium phage FP_Epona OX=2070182 PE=4 SV=1
MM1 pKa = 7.74RR2 pKa = 11.84NAKK5 pKa = 8.02VTAAITAAVAAVLAVLGKK23 pKa = 10.45AFNFGVDD30 pKa = 3.18TTTQILMRR38 pKa = 11.84FGYY41 pKa = 10.34DD42 pKa = 2.55WGRR45 pKa = 11.84AAARR49 pKa = 11.84APFYY53 pKa = 10.67FSLAIALIGLLACTGWIVSEE73 pKa = 4.16DD74 pKa = 3.64ARR76 pKa = 11.84RR77 pKa = 11.84QLWKK81 pKa = 9.4MWSKK85 pKa = 10.49PKK87 pKa = 10.57GYY89 pKa = 11.05GKK91 pKa = 8.54ITRR94 pKa = 11.84NHH96 pKa = 5.81ARR98 pKa = 11.84NPEE101 pKa = 3.94YY102 pKa = 9.9PQQEE106 pKa = 3.96RR107 pKa = 11.84RR108 pKa = 11.84GG109 pKa = 3.72

Molecular weight: 12.15 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

76

0

76

15173

47

1096

199.6

22.22

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.813 ± 0.654

1.397 ± 0.137

6.189 ± 0.248

6.65 ± 0.309

3.552 ± 0.148

6.9 ± 0.3

1.555 ± 0.136

5.997 ± 0.221

6.69 ± 0.327

7.461 ± 0.186

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.794 ± 0.149

4.159 ± 0.168

3.935 ± 0.221

4.047 ± 0.163

5.081 ± 0.316

5.853 ± 0.315

6.676 ± 0.247

6.116 ± 0.277

1.437 ± 0.121

3.697 ± 0.18
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski