Mitsuokella sp. AF33-22

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Negativicutes; Selenomonadales; Selenomonadaceae; Mitsuokella; unclassified Mitsuokella

Average proteome isoelectric point is 6.31

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2221 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A415R5B9|A0A415R5B9_9FIRM Epoxyqueuosine reductase QueH OS=Mitsuokella sp. AF33-22 OX=2292047 GN=queH PE=3 SV=1
MM1 pKa = 7.73ADD3 pKa = 2.87EE4 pKa = 4.63MMNEE8 pKa = 3.91EE9 pKa = 4.25DD10 pKa = 3.99VVVVMTDD17 pKa = 3.23EE18 pKa = 5.46DD19 pKa = 3.67GHH21 pKa = 6.03EE22 pKa = 3.9YY23 pKa = 10.7YY24 pKa = 10.69YY25 pKa = 10.44RR26 pKa = 11.84EE27 pKa = 3.95EE28 pKa = 3.96MIIPVEE34 pKa = 4.05EE35 pKa = 3.59EE36 pKa = 4.12RR37 pKa = 11.84YY38 pKa = 9.34ALLVGIHH45 pKa = 6.55GEE47 pKa = 4.28DD48 pKa = 3.63EE49 pKa = 3.87EE50 pKa = 4.54HH51 pKa = 7.43AAGCGCGCEE60 pKa = 5.45DD61 pKa = 4.24GDD63 pKa = 4.47EE64 pKa = 4.17DD65 pKa = 5.26VIIAKK70 pKa = 9.87IVVNDD75 pKa = 3.81EE76 pKa = 4.05GEE78 pKa = 4.04EE79 pKa = 4.28EE80 pKa = 4.37YY81 pKa = 10.43IEE83 pKa = 4.63PSDD86 pKa = 3.88EE87 pKa = 3.94EE88 pKa = 4.27FEE90 pKa = 4.57AVQKK94 pKa = 10.98AYY96 pKa = 10.54DD97 pKa = 3.77ALVDD101 pKa = 3.86EE102 pKa = 5.31AEE104 pKa = 4.32KK105 pKa = 10.68EE106 pKa = 4.13

Molecular weight: 12.01 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A415R5D3|A0A415R5D3_9FIRM D-amino acid aminotransferase OS=Mitsuokella sp. AF33-22 OX=2292047 GN=DWZ54_09240 PE=4 SV=1
MM1 pKa = 7.93DD2 pKa = 5.59LFHH5 pKa = 7.44RR6 pKa = 11.84YY7 pKa = 8.33AVPLLPAEE15 pKa = 4.11YY16 pKa = 10.06AARR19 pKa = 11.84QDD21 pKa = 3.45SSSVCFEE28 pKa = 4.26LRR30 pKa = 11.84KK31 pKa = 10.74SMDD34 pKa = 3.4LFHH37 pKa = 7.13RR38 pKa = 11.84CAVPLLPAGRR48 pKa = 11.84RR49 pKa = 11.84LGALALRR56 pKa = 11.84QNTSAASRR64 pKa = 11.84AAFPAHH70 pKa = 7.01GEE72 pKa = 4.07GAPKK76 pKa = 10.21GRR78 pKa = 11.84KK79 pKa = 8.92RR80 pKa = 11.84SLNWRR85 pKa = 11.84VPAQLDD91 pKa = 3.86YY92 pKa = 11.06KK93 pKa = 9.81HH94 pKa = 6.1QRR96 pKa = 11.84AAA98 pKa = 3.41

Molecular weight: 10.91 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2221

0

2221

743751

27

3620

334.9

37.12

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.757 ± 0.072

1.289 ± 0.021

5.608 ± 0.049

6.627 ± 0.057

3.995 ± 0.038

7.652 ± 0.051

2.371 ± 0.025

6.313 ± 0.047

5.389 ± 0.047

9.699 ± 0.071

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.961 ± 0.028

3.295 ± 0.046

3.981 ± 0.032

3.54 ± 0.044

5.574 ± 0.061

5.128 ± 0.041

5.368 ± 0.056

7.116 ± 0.042

0.973 ± 0.019

3.364 ± 0.031
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski