Asticcacaulis biprosthecum C19

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Caulobacterales; Caulobacteraceae; Asticcacaulis; Asticcacaulis biprosthecium

Average proteome isoelectric point is 6.4

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4712 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|F4QGL9|F4QGL9_9CAUL Siderophore-interacting family protein OS=Asticcacaulis biprosthecum C19 OX=715226 GN=ABI_21420 PE=4 SV=1
MM1 pKa = 7.89ADD3 pKa = 3.49FTVEE7 pKa = 3.91PVDD10 pKa = 3.84TVNTTTAGTQRR21 pKa = 11.84APEE24 pKa = 3.91ITGLDD29 pKa = 3.09GGGYY33 pKa = 9.29VVTWYY38 pKa = 11.13GNTADD43 pKa = 5.12LGTVLYY49 pKa = 10.07AQVYY53 pKa = 9.13GADD56 pKa = 3.77GDD58 pKa = 4.11KK59 pKa = 11.19LGGEE63 pKa = 4.6VVVADD68 pKa = 4.34HH69 pKa = 6.83LPVTGADD76 pKa = 3.08VAARR80 pKa = 11.84ADD82 pKa = 3.46GGFVVIYY89 pKa = 9.14TEE91 pKa = 4.25AGSAFGIAGDD101 pKa = 3.86PPVIAGRR108 pKa = 11.84HH109 pKa = 4.45FDD111 pKa = 3.41ATGAATSAAYY121 pKa = 9.87AISQAPTLDD130 pKa = 3.6QINPDD135 pKa = 3.23IARR138 pKa = 11.84LADD141 pKa = 3.57GGFMVTWSGQQQASSSDD158 pKa = 3.77HH159 pKa = 6.17NLHH162 pKa = 5.63GQKK165 pKa = 10.62LNLYY169 pKa = 6.84GQRR172 pKa = 11.84VGDD175 pKa = 3.96MQYY178 pKa = 10.75VAANLSYY185 pKa = 10.53QGWAPNPTAGDD196 pKa = 3.4SGTSVIARR204 pKa = 11.84SDD206 pKa = 3.58GGWIVGWMTGRR217 pKa = 11.84SASTAFLTLQAYY229 pKa = 7.34NASGAPVGVAVEE241 pKa = 4.57VKK243 pKa = 9.92PGTYY247 pKa = 9.44PVSSFDD253 pKa = 3.95ADD255 pKa = 3.25ISVLDD260 pKa = 4.38DD261 pKa = 3.36LTVAFNSQSRR271 pKa = 11.84VFIADD276 pKa = 4.25LSTGAVTSFDD286 pKa = 4.4SNVSANTGLLADD298 pKa = 4.08IQGLPDD304 pKa = 3.85GSLLVTWTEE313 pKa = 3.63VDD315 pKa = 3.55GAAGLLKK322 pKa = 10.53AAIYY326 pKa = 10.52DD327 pKa = 3.62RR328 pKa = 11.84AGVVLSDD335 pKa = 3.27IVTVTAAGNNRR346 pKa = 11.84YY347 pKa = 7.97ATATMLSDD355 pKa = 4.13GNVALAWTDD364 pKa = 3.64TVSSDD369 pKa = 3.36IEE371 pKa = 4.15TTIILRR377 pKa = 11.84NHH379 pKa = 5.98GPEE382 pKa = 4.01LTGEE386 pKa = 4.18PVVLPGATEE395 pKa = 3.92DD396 pKa = 3.48TVYY399 pKa = 10.59TLSTADD405 pKa = 4.15LLAGYY410 pKa = 8.12TDD412 pKa = 3.56TDD414 pKa = 3.86GDD416 pKa = 4.24SLSVKK421 pKa = 10.46SLTLEE426 pKa = 4.29GGTLVNNGDD435 pKa = 3.88GTYY438 pKa = 10.52SWTPPANYY446 pKa = 9.42FGPIGLSYY454 pKa = 10.32KK455 pKa = 10.35ISDD458 pKa = 3.72GQGANLIVTRR468 pKa = 11.84SFEE471 pKa = 4.2VARR474 pKa = 11.84VTDD477 pKa = 4.21TIFNSNSTRR486 pKa = 11.84LDD488 pKa = 3.36QYY490 pKa = 11.53SEE492 pKa = 3.9NLTLTGRR499 pKa = 11.84GNIKK503 pKa = 10.52GVGNSWDD510 pKa = 3.55NVLTGNSGINTLEE523 pKa = 4.15GQLGNDD529 pKa = 3.23TYY531 pKa = 11.58YY532 pKa = 10.72VQNSEE537 pKa = 4.09DD538 pKa = 3.97HH539 pKa = 5.66IVEE542 pKa = 4.08YY543 pKa = 11.01RR544 pKa = 11.84NGGVDD549 pKa = 3.77LVYY552 pKa = 11.08ASVDD556 pKa = 3.33YY557 pKa = 11.21SLAGGDD563 pKa = 3.68TEE565 pKa = 5.07YY566 pKa = 11.64LFLTGTADD574 pKa = 3.76LNGRR578 pKa = 11.84GNASANTLAGNGGANRR594 pKa = 11.84LSGDD598 pKa = 3.56RR599 pKa = 11.84GHH601 pKa = 7.44DD602 pKa = 3.48RR603 pKa = 11.84LNGGAGADD611 pKa = 3.74TLIGATGNDD620 pKa = 3.65TYY622 pKa = 11.78VVDD625 pKa = 3.93NVGDD629 pKa = 4.01SVSDD633 pKa = 3.25VDD635 pKa = 4.5EE636 pKa = 4.83NFFSGGTDD644 pKa = 3.17VVEE647 pKa = 5.44ASVSWTLGTDD657 pKa = 3.97LEE659 pKa = 4.35QLVLTGAAAIDD670 pKa = 3.85GTGNGLSNVLTGNDD684 pKa = 3.3ARR686 pKa = 11.84NVLTGGLGNDD696 pKa = 3.27TYY698 pKa = 11.33YY699 pKa = 10.37IQNEE703 pKa = 3.93TDD705 pKa = 3.22RR706 pKa = 11.84VVEE709 pKa = 3.76LHH711 pKa = 6.92FEE713 pKa = 4.39GEE715 pKa = 4.28DD716 pKa = 3.65TVISSVSYY724 pKa = 10.73SLFGRR729 pKa = 11.84AVEE732 pKa = 4.21TLLLTGTGNLNGTGNSLNNTLTGTDD757 pKa = 3.75GNNVLDD763 pKa = 4.67GGTGGDD769 pKa = 4.06LMSGGLGNDD778 pKa = 3.24TYY780 pKa = 11.68YY781 pKa = 11.34VDD783 pKa = 3.96NFYY786 pKa = 11.53DD787 pKa = 3.82NVAEE791 pKa = 4.82LHH793 pKa = 6.09LQGNDD798 pKa = 2.9TVYY801 pKa = 11.51ASVTYY806 pKa = 10.86SFFGRR811 pKa = 11.84AAEE814 pKa = 3.96VLILTGAGNINGTGNSLVNTLIGNSGANVLDD845 pKa = 4.14GAGGNDD851 pKa = 3.68NLTGGAGADD860 pKa = 3.25VFLFSAGSRR869 pKa = 11.84ADD871 pKa = 3.3TVTDD875 pKa = 4.77FNAADD880 pKa = 3.75NDD882 pKa = 4.25SLNVNAYY889 pKa = 8.63TGGVANAGLVSQTGNHH905 pKa = 5.02VVINLGGGNVVTVLNASQAEE925 pKa = 4.35ILAHH929 pKa = 5.2MVWW932 pKa = 3.49

Molecular weight: 95.72 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|F4QN39|F4QN39_9CAUL Tad domain-containing protein OS=Asticcacaulis biprosthecum C19 OX=715226 GN=ABI_30470 PE=4 SV=1
MM1 pKa = 8.17RR2 pKa = 11.84YY3 pKa = 10.03LSFLSRR9 pKa = 11.84RR10 pKa = 11.84RR11 pKa = 11.84RR12 pKa = 11.84FTSRR16 pKa = 11.84ILRR19 pKa = 11.84RR20 pKa = 11.84KK21 pKa = 9.2GLHH24 pKa = 5.46TRR26 pKa = 11.84LILQVGSARR35 pKa = 11.84QRR37 pKa = 11.84RR38 pKa = 11.84KK39 pKa = 9.93RR40 pKa = 3.46

Molecular weight: 5.03 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4712

0

4712

1501214

37

4917

318.6

34.49

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.699 ± 0.052

0.8 ± 0.013

6.362 ± 0.048

5.047 ± 0.032

3.77 ± 0.026

8.87 ± 0.09

1.992 ± 0.019

4.963 ± 0.022

3.703 ± 0.033

9.693 ± 0.041

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.368 ± 0.019

3.244 ± 0.042

4.829 ± 0.038

3.363 ± 0.023

6.103 ± 0.049

5.658 ± 0.032

5.907 ± 0.042

7.315 ± 0.031

1.435 ± 0.017

2.684 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski