Rhizobiales bacterium PAR1

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales; unclassified Hyphomicrobiales

Average proteome isoelectric point is 6.96

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3826 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A257HC63|A0A257HC63_9RHIZ Mandelate racemase OS=Rhizobiales bacterium PAR1 OX=2015577 GN=CFE31_14540 PE=4 SV=1
MM1 pKa = 7.58AWDD4 pKa = 4.73LYY6 pKa = 11.38EE7 pKa = 6.14DD8 pKa = 4.35GASLGTAGEE17 pKa = 4.45DD18 pKa = 3.14GGTIVADD25 pKa = 4.54FEE27 pKa = 5.62HH28 pKa = 7.44DD29 pKa = 3.15LGARR33 pKa = 11.84MTLEE37 pKa = 3.91ALGDD41 pKa = 4.0GTCFAMTCGIYY52 pKa = 10.34GWFFHH57 pKa = 6.04TRR59 pKa = 11.84FFNSRR64 pKa = 11.84EE65 pKa = 3.9EE66 pKa = 4.07ADD68 pKa = 3.75RR69 pKa = 11.84ATVDD73 pKa = 3.24MQSALNVILQSYY85 pKa = 7.18PAKK88 pKa = 10.64DD89 pKa = 3.57DD90 pKa = 3.77ADD92 pKa = 3.77YY93 pKa = 11.13DD94 pKa = 3.93AKK96 pKa = 10.59TEE98 pKa = 4.0AFGDD102 pKa = 4.29AISAFVDD109 pKa = 4.5AYY111 pKa = 10.01PP112 pKa = 4.46

Molecular weight: 12.19 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A257HCR4|A0A257HCR4_9RHIZ C4-dicarboxylate ABC transporter permease OS=Rhizobiales bacterium PAR1 OX=2015577 GN=CFE31_13705 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 10.27QPSKK9 pKa = 9.73LVRR12 pKa = 11.84KK13 pKa = 9.15RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.42GFRR19 pKa = 11.84ARR21 pKa = 11.84MATVGGRR28 pKa = 11.84KK29 pKa = 9.32VIAARR34 pKa = 11.84RR35 pKa = 11.84NRR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 9.0KK41 pKa = 10.58LSAA44 pKa = 4.03

Molecular weight: 5.15 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3826

0

3826

1256189

34

4703

328.3

35.5

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.732 ± 0.045

0.809 ± 0.013

5.147 ± 0.032

5.635 ± 0.038

3.928 ± 0.025

8.666 ± 0.049

1.951 ± 0.018

5.661 ± 0.026

3.853 ± 0.033

10.226 ± 0.048

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.627 ± 0.018

2.633 ± 0.028

5.207 ± 0.036

2.905 ± 0.023

6.705 ± 0.04

5.335 ± 0.029

5.42 ± 0.039

7.294 ± 0.03

1.18 ± 0.016

2.086 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski