Leucobacter sp. 7(1)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micrococcales; Microbacteriaceae; Leucobacter; unclassified Leucobacter

Average proteome isoelectric point is 6.08

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3533 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1R4HKK8|A0A1R4HKK8_9MICO Putative conserved integral membrane protein OS=Leucobacter sp. 7(1) OX=1255613 GN=FM113_00670 PE=4 SV=1
MM1 pKa = 7.07RR2 pKa = 11.84TSVLAVGALATLALGLVGCSAGTPGDD28 pKa = 3.7APAEE32 pKa = 4.12EE33 pKa = 4.55SSAWPEE39 pKa = 4.33SITLSLVPSVEE50 pKa = 4.15GEE52 pKa = 4.29DD53 pKa = 3.58LAEE56 pKa = 4.59ALDD59 pKa = 4.17PLTTYY64 pKa = 10.86LSEE67 pKa = 3.93QLGIEE72 pKa = 4.26VEE74 pKa = 4.4GVVANDD80 pKa = 3.24YY81 pKa = 10.89AATVEE86 pKa = 5.11ALGADD91 pKa = 3.58QAQVIISDD99 pKa = 3.69AGSLYY104 pKa = 10.49QAMEE108 pKa = 3.93QYY110 pKa = 10.59DD111 pKa = 4.16AEE113 pKa = 4.41LVLRR117 pKa = 11.84DD118 pKa = 3.56VRR120 pKa = 11.84FGATSYY126 pKa = 11.31AAVGYY131 pKa = 8.0TNNPDD136 pKa = 3.75KK137 pKa = 11.32YY138 pKa = 10.64CADD141 pKa = 3.82TPVMAKK147 pKa = 10.08YY148 pKa = 9.8AASDD152 pKa = 3.66IEE154 pKa = 4.6LSYY157 pKa = 11.75CNGTEE162 pKa = 3.93QAGVDD167 pKa = 3.58ASGAGPAGLEE177 pKa = 4.03SLKK180 pKa = 11.05KK181 pKa = 9.6IDD183 pKa = 4.44AGTKK187 pKa = 9.52VALQAATSPAGYY199 pKa = 9.58QYY201 pKa = 10.52PIVAMRR207 pKa = 11.84DD208 pKa = 3.05ARR210 pKa = 11.84INTDD214 pKa = 2.99TGITQVPVEE223 pKa = 4.28GNNNAVLAVANGDD236 pKa = 3.74AEE238 pKa = 4.5VSFGYY243 pKa = 9.78WDD245 pKa = 3.55ARR247 pKa = 11.84TTVTAEE253 pKa = 4.03APDD256 pKa = 3.43IAEE259 pKa = 4.18KK260 pKa = 10.8VVAFAYY266 pKa = 9.84TEE268 pKa = 4.19MIPNGGVAVAPTLPADD284 pKa = 4.32LVAEE288 pKa = 4.41LTEE291 pKa = 4.51LMAGYY296 pKa = 10.62ADD298 pKa = 3.56SSEE301 pKa = 4.13EE302 pKa = 3.92AKK304 pKa = 10.06TVMFDD309 pKa = 4.33LVGLSGWTDD318 pKa = 2.97KK319 pKa = 10.56TASEE323 pKa = 4.26EE324 pKa = 3.69IARR327 pKa = 11.84YY328 pKa = 10.23GEE330 pKa = 3.99ILSEE334 pKa = 4.05FAKK337 pKa = 10.88

Molecular weight: 34.9 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1R4HVY9|A0A1R4HVY9_9MICO Urocanate hydratase OS=Leucobacter sp. 7(1) OX=1255613 GN=hutU PE=3 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84AKK15 pKa = 9.5VHH17 pKa = 5.46GFRR20 pKa = 11.84ARR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AIVAARR35 pKa = 11.84RR36 pKa = 11.84GKK38 pKa = 10.45GRR40 pKa = 11.84SKK42 pKa = 10.13LTAA45 pKa = 4.04

Molecular weight: 5.18 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3533

0

3533

1161179

37

2469

328.7

35.09

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.669 ± 0.059

0.544 ± 0.009

5.537 ± 0.041

5.997 ± 0.049

3.19 ± 0.025

9.004 ± 0.043

2.029 ± 0.018

4.638 ± 0.026

1.884 ± 0.033

10.324 ± 0.055

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.833 ± 0.016

2.028 ± 0.023

5.554 ± 0.039

3.052 ± 0.024

7.044 ± 0.044

5.665 ± 0.026

6.342 ± 0.039

8.393 ± 0.042

1.416 ± 0.017

1.857 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski