Hubei orthoptera virus 4

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 7.4

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A1L3KH43|A0A1L3KH43_9VIRU Capsid protein OS=Hubei orthoptera virus 4 OX=1923012 PE=4 SV=1
MM1 pKa = 7.65SPTTPKK7 pKa = 10.09MSAKK11 pKa = 9.09TLRR14 pKa = 11.84NRR16 pKa = 11.84RR17 pKa = 11.84KK18 pKa = 9.61RR19 pKa = 11.84LARR22 pKa = 11.84KK23 pKa = 8.98ARR25 pKa = 11.84QTAANSQLLPGIGYY39 pKa = 7.33NTNRR43 pKa = 11.84NGVAPSRR50 pKa = 11.84ADD52 pKa = 3.06PRR54 pKa = 11.84TFNLLNNLGNTIDD67 pKa = 4.49GRR69 pKa = 11.84AACMKK74 pKa = 9.84ILHH77 pKa = 6.85PCMEE81 pKa = 4.66TGNHH85 pKa = 4.5VTKK88 pKa = 10.65FPDD91 pKa = 3.86GAVSTSVVLEE101 pKa = 3.86RR102 pKa = 11.84RR103 pKa = 11.84DD104 pKa = 3.78EE105 pKa = 4.18YY106 pKa = 11.27SIPPPKK112 pKa = 10.67DD113 pKa = 3.34FGAVKK118 pKa = 10.72LEE120 pKa = 4.01TWSCLVFHH128 pKa = 6.96LPFAMCRR135 pKa = 11.84TVVCRR140 pKa = 11.84YY141 pKa = 8.97HH142 pKa = 6.79AALPVSEE149 pKa = 4.37QRR151 pKa = 11.84VEE153 pKa = 3.87TALAAMVALFLDD165 pKa = 3.41QDD167 pKa = 3.82HH168 pKa = 6.52NKK170 pKa = 10.52EE171 pKa = 4.02YY172 pKa = 10.07IYY174 pKa = 10.36PNWTDD179 pKa = 3.37KK180 pKa = 11.38DD181 pKa = 3.28IGPTDD186 pKa = 3.8NGNISQWSVLTSDD199 pKa = 3.49TLTPEE204 pKa = 4.37LLLNSGSDD212 pKa = 3.4VMRR215 pKa = 11.84SIRR218 pKa = 11.84RR219 pKa = 11.84TCMGVTVDD227 pKa = 5.13LDD229 pKa = 3.88APALANQGRR238 pKa = 11.84VITGQWGPDD247 pKa = 3.23VALGTLSFNKK257 pKa = 10.22DD258 pKa = 2.88NAVEE262 pKa = 3.97VRR264 pKa = 11.84DD265 pKa = 4.19IYY267 pKa = 11.04DD268 pKa = 3.41INDD271 pKa = 4.05PAWTTNAIVSSDD283 pKa = 3.61EE284 pKa = 4.11FCRR287 pKa = 11.84QAQAKK292 pKa = 8.32YY293 pKa = 10.89GSYY296 pKa = 10.17TPIRR300 pKa = 11.84PIGLDD305 pKa = 3.41VPFTPITEE313 pKa = 3.96KK314 pKa = 10.63RR315 pKa = 11.84VISMRR320 pKa = 11.84SNLAGINFDD329 pKa = 3.57TVEE332 pKa = 4.1AFGDD336 pKa = 3.25LWLRR340 pKa = 11.84GWCTAVEE347 pKa = 4.3SWDD350 pKa = 3.45GMDD353 pKa = 3.64RR354 pKa = 11.84SASLRR359 pKa = 11.84MKK361 pKa = 9.99IRR363 pKa = 11.84EE364 pKa = 4.59DD365 pKa = 3.33IEE367 pKa = 5.56LIAAPKK373 pKa = 10.17SPYY376 pKa = 10.55SPFSTPALPDD386 pKa = 3.62DD387 pKa = 3.73LRR389 pKa = 11.84ARR391 pKa = 11.84TMIQEE396 pKa = 4.44FSRR399 pKa = 11.84TQPHH403 pKa = 7.0SYY405 pKa = 9.37PAEE408 pKa = 4.27DD409 pKa = 3.65NEE411 pKa = 4.59FGKK414 pKa = 10.67LVPKK418 pKa = 10.12ILSGIAGALSNLGIPVVSPIASVVGNVADD447 pKa = 3.77SAFGKK452 pKa = 9.53MVGDD456 pKa = 3.66WLDD459 pKa = 3.24KK460 pKa = 11.25LFF462 pKa = 4.86

Molecular weight:
50.87 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A1L3KH43|A0A1L3KH43_9VIRU Capsid protein OS=Hubei orthoptera virus 4 OX=1923012 PE=4 SV=1
MM1 pKa = 7.56TKK3 pKa = 9.71QSNYY7 pKa = 8.54IQHH10 pKa = 7.19DD11 pKa = 3.76VLPRR15 pKa = 11.84DD16 pKa = 4.09GQDD19 pKa = 3.23PSPWAMVAKK28 pKa = 10.56HH29 pKa = 5.22MFLRR33 pKa = 11.84VYY35 pKa = 10.21HH36 pKa = 6.6IIWFIVYY43 pKa = 7.7LVCNCGFPTVRR54 pKa = 11.84EE55 pKa = 3.79TSEE58 pKa = 3.62WVEE61 pKa = 4.03AVITRR66 pKa = 11.84PKK68 pKa = 10.32NLRR71 pKa = 11.84HH72 pKa = 6.05LFLNTEE78 pKa = 3.95VMSIFPCRR86 pKa = 11.84TSHH89 pKa = 6.03SHH91 pKa = 6.02PTSAMYY97 pKa = 8.37RR98 pKa = 11.84TAVNNFMNTLATRR111 pKa = 11.84AGMEE115 pKa = 4.11PFNVSKK121 pKa = 11.08SPTDD125 pKa = 3.26GGKK128 pKa = 8.12GTRR131 pKa = 11.84YY132 pKa = 9.81FYY134 pKa = 10.74GAKK137 pKa = 10.25DD138 pKa = 3.29LATPYY143 pKa = 10.83VDD145 pKa = 5.65DD146 pKa = 4.25KK147 pKa = 11.75VGNNDD152 pKa = 3.75VIIMCDD158 pKa = 2.72VDD160 pKa = 4.3YY161 pKa = 10.19YY162 pKa = 11.43TDD164 pKa = 3.38MTKK167 pKa = 9.7WLSYY171 pKa = 9.75FRR173 pKa = 11.84PILMYY178 pKa = 10.21TFVPTSAAARR188 pKa = 11.84RR189 pKa = 11.84KK190 pKa = 10.0DD191 pKa = 3.42YY192 pKa = 10.74AYY194 pKa = 11.04RR195 pKa = 11.84LIDD198 pKa = 3.52DD199 pKa = 3.72HH200 pKa = 9.58VEE202 pKa = 3.74FHH204 pKa = 6.31VAGGSSYY211 pKa = 10.37RR212 pKa = 11.84HH213 pKa = 6.11KK214 pKa = 10.58LWNYY218 pKa = 8.61KK219 pKa = 9.77GDD221 pKa = 3.89YY222 pKa = 10.33VSTVDD227 pKa = 4.96DD228 pKa = 4.14SGNLLVFHH236 pKa = 6.87LEE238 pKa = 3.63QRR240 pKa = 11.84NIIGDD245 pKa = 3.63PDD247 pKa = 3.11HH248 pKa = 7.25RR249 pKa = 11.84YY250 pKa = 9.45IVLTPLAKK258 pKa = 10.01IPSPFHH264 pKa = 5.53WFLPKK269 pKa = 10.16FEE271 pKa = 5.44PIRR274 pKa = 11.84RR275 pKa = 11.84HH276 pKa = 6.42SFLQDD281 pKa = 3.22GTSVLYY287 pKa = 10.42EE288 pKa = 4.63PINDD292 pKa = 4.2TLSLAPNGAWHH303 pKa = 6.49SVDD306 pKa = 3.81ILGTQYY312 pKa = 11.47SAIKK316 pKa = 10.09QRR318 pKa = 11.84LLNKK322 pKa = 9.77SAPPVMSDD330 pKa = 3.0IEE332 pKa = 4.17RR333 pKa = 11.84LLRR336 pKa = 11.84ASGDD340 pKa = 3.56EE341 pKa = 4.04NAVTNAPLIFNLMHH355 pKa = 7.07LNFHH359 pKa = 6.56KK360 pKa = 10.96NVVLTNGVVSSFQPLGSLATEE381 pKa = 4.65DD382 pKa = 4.29GKK384 pKa = 10.15PSGKK388 pKa = 9.59QLTPPLVSEE397 pKa = 4.27PAVFPTKK404 pKa = 10.4GVNADD409 pKa = 3.14EE410 pKa = 4.18ATIKK414 pKa = 10.87GRR416 pKa = 11.84ITSVANPVVPPRR428 pKa = 11.84KK429 pKa = 9.74YY430 pKa = 10.37KK431 pKa = 10.68DD432 pKa = 3.45YY433 pKa = 11.54ANEE436 pKa = 3.85FTNCIVKK443 pKa = 10.38RR444 pKa = 11.84AGTGSPLSVDD454 pKa = 4.07EE455 pKa = 4.43VRR457 pKa = 11.84KK458 pKa = 9.08LQPNPQQKK466 pKa = 10.28ARR468 pKa = 11.84FNKK471 pKa = 9.51VEE473 pKa = 4.2ATLTTTPQNDD483 pKa = 3.09LKK485 pKa = 11.46AFIKK489 pKa = 10.61SEE491 pKa = 4.02AYY493 pKa = 10.59GNVTDD498 pKa = 4.75PRR500 pKa = 11.84NITTMSPEE508 pKa = 4.15LTVMLSGFTYY518 pKa = 10.65AFKK521 pKa = 10.6QDD523 pKa = 3.51CLKK526 pKa = 9.62NQKK529 pKa = 9.01WYY531 pKa = 10.92GPGKK535 pKa = 9.02TPTQTLKK542 pKa = 11.23ALAALSDD549 pKa = 5.35LSDD552 pKa = 2.95EE553 pKa = 4.37WLTVDD558 pKa = 4.42YY559 pKa = 11.15SRR561 pKa = 11.84LDD563 pKa = 3.44GTVSEE568 pKa = 4.42FLQRR572 pKa = 11.84SVVMATYY579 pKa = 9.62LKK581 pKa = 10.03WVAEE585 pKa = 4.02EE586 pKa = 4.43HH587 pKa = 6.27KK588 pKa = 11.17AEE590 pKa = 4.49LKK592 pKa = 10.86HH593 pKa = 5.66EE594 pKa = 4.3LEE596 pKa = 5.14AIFIKK601 pKa = 10.41SGRR604 pKa = 11.84TANGVKK610 pKa = 10.21FNPGYY615 pKa = 8.45GTRR618 pKa = 11.84SGSPITTDD626 pKa = 3.32GNTMICAYY634 pKa = 10.14VVYY637 pKa = 10.56CCFRR641 pKa = 11.84EE642 pKa = 4.08LGYY645 pKa = 10.49KK646 pKa = 9.85PKK648 pKa = 10.51EE649 pKa = 4.04AFDD652 pKa = 4.62SIGLVYY658 pKa = 10.62GDD660 pKa = 5.23DD661 pKa = 4.01GAFPALPGLKK671 pKa = 9.55EE672 pKa = 3.8KK673 pKa = 11.39LEE675 pKa = 4.18IVSKK679 pKa = 10.86QLGLKK684 pKa = 9.94IKK686 pKa = 10.72LDD688 pKa = 3.52VSKK691 pKa = 11.06VGEE694 pKa = 4.04PLPYY698 pKa = 10.23LGRR701 pKa = 11.84FFVDD705 pKa = 4.19PKK707 pKa = 8.81TCKK710 pKa = 10.32DD711 pKa = 3.41SFQDD715 pKa = 4.07PIRR718 pKa = 11.84TISKK722 pKa = 9.83LHH724 pKa = 5.18LTANKK729 pKa = 9.04TVTPEE734 pKa = 3.27QALANKK740 pKa = 9.45AHH742 pKa = 7.1GYY744 pKa = 9.69LATDD748 pKa = 3.64SKK750 pKa = 10.34TPIIGTWAAKK760 pKa = 10.19VIEE763 pKa = 4.06LTGRR767 pKa = 11.84KK768 pKa = 7.39TKK770 pKa = 10.69GLLRR774 pKa = 11.84EE775 pKa = 4.18EE776 pKa = 4.17AFKK779 pKa = 11.1LSNAWPQANKK789 pKa = 10.79SLITEE794 pKa = 4.56AMAKK798 pKa = 10.18VLDD801 pKa = 4.15LQLSEE806 pKa = 6.35LIALDD811 pKa = 3.44QKK813 pKa = 10.9INQVTALDD821 pKa = 3.8QLPVLLEE828 pKa = 4.14TVRR831 pKa = 11.84EE832 pKa = 4.18TKK834 pKa = 10.28IPAVLGDD841 pKa = 3.76EE842 pKa = 4.34VVEE845 pKa = 4.49PGLHH849 pKa = 5.31VKK851 pKa = 10.42SIEE854 pKa = 4.19SKK856 pKa = 8.98QVKK859 pKa = 8.37DD860 pKa = 3.99TKK862 pKa = 10.69PKK864 pKa = 10.89DD865 pKa = 3.29VDD867 pKa = 3.5APTTGAADD875 pKa = 3.75SVPSLDD881 pKa = 4.26KK882 pKa = 10.94DD883 pKa = 4.26SKK885 pKa = 11.0CSCQTSSITTAGKK898 pKa = 10.23ASNVRR903 pKa = 11.84GNVRR907 pKa = 11.84QNGDD911 pKa = 2.87GAGRR915 pKa = 11.84GGRR918 pKa = 11.84GHH920 pKa = 6.62GPSARR925 pKa = 11.84FRR927 pKa = 11.84SRR929 pKa = 11.84RR930 pKa = 11.84GNQHH934 pKa = 5.02SAKK937 pKa = 9.75PDD939 pKa = 3.5LRR941 pKa = 11.84SGNSNGSPLKK951 pKa = 10.44SDD953 pKa = 3.45LNTRR957 pKa = 11.84EE958 pKa = 3.81KK959 pKa = 9.77TAEE962 pKa = 3.95RR963 pKa = 11.84VV964 pKa = 3.25

Molecular weight:
106.91 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

1426

462

964

713.0

78.89

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.784 ± 0.601

1.403 ± 0.181

6.241 ± 0.378

4.348 ± 0.249

3.647 ± 0.101

6.452 ± 0.096

2.034 ± 0.405

4.769 ± 0.354

6.311 ± 1.212

8.766 ± 0.179

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.104 ± 0.391

5.049 ± 0.2

6.522 ± 0.223

2.805 ± 0.234

5.54 ± 0.645

7.153 ± 0.114

7.363 ± 0.241

7.223 ± 0.163

1.473 ± 0.262

3.015 ± 0.469

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski