Psychrobacter phage pOW20-A

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; unclassified Myoviridae

Average proteome isoelectric point is 5.98

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 71 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|M4SN73|M4SN73_9CAUD Uncharacterized protein OS=Psychrobacter phage pOW20-A OX=754048 GN=PSYG_00022 PE=4 SV=1
MM1 pKa = 6.69TQQIRR6 pKa = 11.84LDD8 pKa = 3.41NDD10 pKa = 3.39ADD12 pKa = 3.97HH13 pKa = 7.26EE14 pKa = 4.33ISEE17 pKa = 4.65SGTSGTVYY25 pKa = 10.14IAMLNDD31 pKa = 3.26VSVEE35 pKa = 4.08IYY37 pKa = 10.85FEE39 pKa = 4.01TDD41 pKa = 2.53DD42 pKa = 3.89RR43 pKa = 11.84QNINLLSVGDD53 pKa = 4.3CFQTINGIDD62 pKa = 3.62EE63 pKa = 4.82DD64 pKa = 4.21YY65 pKa = 11.03AYY67 pKa = 9.6TLQFEE72 pKa = 4.67HH73 pKa = 6.49EE74 pKa = 4.39QPFKK78 pKa = 11.25AVINQFLHH86 pKa = 7.28DD87 pKa = 4.4NPIDD91 pKa = 4.65DD92 pKa = 4.22GDD94 pKa = 3.76EE95 pKa = 4.26YY96 pKa = 11.33EE97 pKa = 5.21PDD99 pKa = 2.73WHH101 pKa = 6.77DD102 pKa = 3.17QRR104 pKa = 11.84TYY106 pKa = 11.43GLSNSDD112 pKa = 4.21FIYY115 pKa = 10.81GG116 pKa = 3.51

Molecular weight: 13.39 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|M4SNA1|M4SNA1_9CAUD Uncharacterized protein OS=Psychrobacter phage pOW20-A OX=754048 GN=PSYG_00057 PE=4 SV=1
MM1 pKa = 6.86TAIIRR6 pKa = 11.84RR7 pKa = 11.84RR8 pKa = 11.84DD9 pKa = 3.17AGALNRR15 pKa = 11.84IIDD18 pKa = 3.95NVRR21 pKa = 11.84SMGLNKK27 pKa = 9.79VEE29 pKa = 4.19VGLPSGGRR37 pKa = 11.84HH38 pKa = 5.92SGTDD42 pKa = 3.38LSMHH46 pKa = 6.17EE47 pKa = 4.49LGMVHH52 pKa = 7.26EE53 pKa = 4.98YY54 pKa = 11.16GSPTRR59 pKa = 11.84NIPPRR64 pKa = 11.84PFIGPPIADD73 pKa = 3.57NVDD76 pKa = 3.34KK77 pKa = 10.97YY78 pKa = 11.26KK79 pKa = 11.07KK80 pKa = 10.07IMRR83 pKa = 11.84KK84 pKa = 7.98QAARR88 pKa = 11.84LLFRR92 pKa = 11.84RR93 pKa = 11.84TSLHH97 pKa = 5.85NALSLVGEE105 pKa = 4.37AGKK108 pKa = 10.71ADD110 pKa = 3.2IQKK113 pKa = 10.79YY114 pKa = 8.03MLSANFKK121 pKa = 9.26PLAASTIEE129 pKa = 4.27AKK131 pKa = 10.69GSSKK135 pKa = 10.5PLIDD139 pKa = 3.69SGQMRR144 pKa = 11.84NAITYY149 pKa = 7.71EE150 pKa = 3.75ITRR153 pKa = 4.0

Molecular weight: 16.83 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

71

0

71

12711

52

523

179.0

19.93

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.135 ± 0.442

1.046 ± 0.14

7.143 ± 0.252

6.042 ± 0.191

3.556 ± 0.18

7.285 ± 0.275

1.691 ± 0.152

6.75 ± 0.242

6.42 ± 0.332

8.072 ± 0.236

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.919 ± 0.142

5.098 ± 0.272

3.139 ± 0.157

4.532 ± 0.274

4.343 ± 0.243

6.593 ± 0.226

6.073 ± 0.276

6.372 ± 0.215

1.495 ± 0.128

3.296 ± 0.182
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski