Microbacterium phage Squash

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Squashvirus; Microbacterium virus Squash

Average proteome isoelectric point is 5.98

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 108 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2U8ULU7|A0A2U8ULU7_9CAUD Uncharacterized protein OS=Microbacterium phage Squash OX=2182357 GN=101 PE=4 SV=1
MM1 pKa = 7.55SRR3 pKa = 11.84STWVDD8 pKa = 3.19HH9 pKa = 6.89AARR12 pKa = 11.84AAAAVALAALMVGGVVALADD32 pKa = 4.22LADD35 pKa = 4.56EE36 pKa = 4.69PLAPCTPTSTSVCSGPPADD55 pKa = 4.44HH56 pKa = 6.66SPNGGEE62 pKa = 4.12YY63 pKa = 10.4DD64 pKa = 4.08SLPPCPTEE72 pKa = 5.13DD73 pKa = 4.1SVGCRR78 pKa = 11.84WDD80 pKa = 3.51ASEE83 pKa = 3.89QGNGEE88 pKa = 3.79GHH90 pKa = 7.06DD91 pKa = 3.9VVNQPP96 pKa = 4.12

Molecular weight: 9.68 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2U8UM50|A0A2U8UM50_9CAUD Uncharacterized protein OS=Microbacterium phage Squash OX=2182357 GN=74 PE=4 SV=1
MM1 pKa = 7.25TSLRR5 pKa = 11.84KK6 pKa = 8.35ITKK9 pKa = 9.96LEE11 pKa = 3.81AAAYY15 pKa = 8.04QASQAATQAVVLARR29 pKa = 11.84MDD31 pKa = 3.67GATEE35 pKa = 4.0AEE37 pKa = 4.18IARR40 pKa = 11.84LSRR43 pKa = 11.84RR44 pKa = 11.84AQAHH48 pKa = 4.6RR49 pKa = 11.84TQWLGLASRR58 pKa = 11.84LEE60 pKa = 4.24LADD63 pKa = 3.4QRR65 pKa = 11.84AEE67 pKa = 4.07RR68 pKa = 11.84VHH70 pKa = 6.24SS71 pKa = 3.9

Molecular weight: 7.83 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

108

0

108

20004

27

1084

185.2

20.15

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.848 ± 0.325

0.98 ± 0.119

6.494 ± 0.193

6.634 ± 0.346

2.734 ± 0.142

8.473 ± 0.262

1.755 ± 0.178

4.869 ± 0.168

2.859 ± 0.226

7.499 ± 0.308

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.2 ± 0.125

2.819 ± 0.173

5.789 ± 0.331

3.789 ± 0.377

6.894 ± 0.284

5.434 ± 0.31

7.194 ± 0.341

7.264 ± 0.233

1.9 ± 0.167

2.574 ± 0.154
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski