Drosophila x virus (isolate Chung/1996) (DXV)

Taxonomy: Viruses; Riboviria; Orthornavirae; Birnaviridae; Entomobirnavirus; Drosophila X virus

Average proteome isoelectric point is 7.42

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|Q96724|POLS_DXV96 Structural polyprotein OS=Drosophila x virus (isolate Chung/1996) OX=654931 PE=1 SV=1
MM1 pKa = 7.01NTTNEE6 pKa = 4.03YY7 pKa = 10.89LKK9 pKa = 10.04TLLNPAQFISDD20 pKa = 3.74IPDD23 pKa = 4.8DD24 pKa = 3.62IMIRR28 pKa = 11.84HH29 pKa = 5.8VNSAQTITYY38 pKa = 8.65NLKK41 pKa = 10.44SGASGTGLIVVYY53 pKa = 9.91PNTPSSISGFHH64 pKa = 6.24YY65 pKa = 10.09IWDD68 pKa = 3.92SATSNWVFDD77 pKa = 3.51QYY79 pKa = 11.35IYY81 pKa = 10.05TAQEE85 pKa = 3.96LKK87 pKa = 10.76DD88 pKa = 3.6SYY90 pKa = 11.69DD91 pKa = 3.46YY92 pKa = 11.68GRR94 pKa = 11.84LISGSLSIKK103 pKa = 10.52SSTLPAGVYY112 pKa = 10.3ALNGTFNAVWFQGTLSEE129 pKa = 4.34VSDD132 pKa = 3.82YY133 pKa = 11.36SYY135 pKa = 11.85DD136 pKa = 4.06RR137 pKa = 11.84ILSITSNPLDD147 pKa = 3.22KK148 pKa = 11.14VGNVLVGDD156 pKa = 4.71GIEE159 pKa = 4.23VLSLPQGFNNPYY171 pKa = 9.84VRR173 pKa = 11.84LGDD176 pKa = 3.89KK177 pKa = 10.86SPSTLSSPTHH187 pKa = 5.61ITNTSQNLATGGAYY201 pKa = 8.02MIPVTTVPGQGFHH214 pKa = 6.53NKK216 pKa = 8.91EE217 pKa = 3.41FSINVDD223 pKa = 3.02SVGPVDD229 pKa = 4.5ILWSGQMTMQDD240 pKa = 3.61EE241 pKa = 4.59WTVTANYY248 pKa = 9.97QPLNISGTLIANSQRR263 pKa = 11.84TLTWSNTGVSNGSHH277 pKa = 5.22YY278 pKa = 11.12MNMNNLNVSLFHH290 pKa = 6.83EE291 pKa = 4.91NPPPEE296 pKa = 4.28PVAAIKK302 pKa = 10.66ININYY307 pKa = 9.06GNNTNGDD314 pKa = 3.49SSFSVDD320 pKa = 3.06SSFTINVIGGATIGVNSPTVGVGYY344 pKa = 10.46QGVAEE349 pKa = 4.54GTAITISGINNYY361 pKa = 9.17EE362 pKa = 4.12LVPNPDD368 pKa = 3.36LQKK371 pKa = 10.7NLPMTYY377 pKa = 8.93GTCDD381 pKa = 3.48PHH383 pKa = 8.25DD384 pKa = 3.86LTYY387 pKa = 10.63IKK389 pKa = 10.65YY390 pKa = 9.22ILSNRR395 pKa = 11.84EE396 pKa = 3.65QLGLRR401 pKa = 11.84SVMTLADD408 pKa = 3.91YY409 pKa = 11.54NRR411 pKa = 11.84MKK413 pKa = 10.18MYY415 pKa = 9.53MHH417 pKa = 7.05VLTNYY422 pKa = 10.13HH423 pKa = 4.97VDD425 pKa = 3.28EE426 pKa = 5.43RR427 pKa = 11.84EE428 pKa = 3.89ASSFDD433 pKa = 2.97FWQLLKK439 pKa = 10.56QIKK442 pKa = 9.58NVAVPLAATLAPQFAPIIGAADD464 pKa = 3.71GLANAILGDD473 pKa = 4.09SASGRR478 pKa = 11.84PVGNSASGMPISMSRR493 pKa = 11.84RR494 pKa = 11.84LRR496 pKa = 11.84NAYY499 pKa = 10.1SADD502 pKa = 3.53SPLGEE507 pKa = 4.32EE508 pKa = 3.44HH509 pKa = 7.09WLPNEE514 pKa = 3.89NEE516 pKa = 4.18NFNKK520 pKa = 9.81FDD522 pKa = 3.87IIYY525 pKa = 9.42DD526 pKa = 3.66VSHH529 pKa = 7.6SSMALFPVIMMEE541 pKa = 3.88HH542 pKa = 7.1DD543 pKa = 3.58KK544 pKa = 11.15VIPSDD549 pKa = 3.77PEE551 pKa = 3.51EE552 pKa = 4.86LYY554 pKa = 10.54IAVSLTEE561 pKa = 4.17SLRR564 pKa = 11.84KK565 pKa = 9.69QIPNLNDD572 pKa = 2.91MPYY575 pKa = 10.41YY576 pKa = 10.56EE577 pKa = 4.57MGGHH581 pKa = 5.96RR582 pKa = 11.84VYY584 pKa = 11.33NSVSSNVRR592 pKa = 11.84SGNFLRR598 pKa = 11.84SDD600 pKa = 4.3YY601 pKa = 11.03ILLPCYY607 pKa = 9.93QLLEE611 pKa = 4.28GRR613 pKa = 11.84LASSTSPNKK622 pKa = 9.21VTGTSHH628 pKa = 6.38QLAIYY633 pKa = 9.89AADD636 pKa = 4.45DD637 pKa = 3.97LLKK640 pKa = 11.07SGVLGKK646 pKa = 10.71APFAAFTGSVVGSSVGEE663 pKa = 3.75VFGINLKK670 pKa = 10.4LQLTDD675 pKa = 3.33SLGIPLLGNSPGLVQVKK692 pKa = 8.33TLTSLDD698 pKa = 3.76KK699 pKa = 10.7KK700 pKa = 10.23IKK702 pKa = 10.83DD703 pKa = 3.22MGDD706 pKa = 3.08VKK708 pKa = 10.77RR709 pKa = 11.84RR710 pKa = 11.84TPKK713 pKa = 8.76QTLPHH718 pKa = 5.61WTAGSASMNPFMNTNPFLEE737 pKa = 4.97EE738 pKa = 4.1LDD740 pKa = 3.66QPIPSNAAKK749 pKa = 10.02PISEE753 pKa = 4.24EE754 pKa = 3.98TRR756 pKa = 11.84DD757 pKa = 3.74LFLSDD762 pKa = 3.45GQTIPSSQEE771 pKa = 3.54KK772 pKa = 9.63IATIHH777 pKa = 6.63EE778 pKa = 4.3YY779 pKa = 10.91LLEE782 pKa = 4.54HH783 pKa = 6.96KK784 pKa = 9.81EE785 pKa = 3.86LEE787 pKa = 4.1EE788 pKa = 4.88AMFSLISQGRR798 pKa = 11.84GRR800 pKa = 11.84SLINMVVKK808 pKa = 10.54SALNIEE814 pKa = 4.43TQSRR818 pKa = 11.84EE819 pKa = 3.85VTGEE823 pKa = 3.19RR824 pKa = 11.84RR825 pKa = 11.84QRR827 pKa = 11.84LEE829 pKa = 3.63RR830 pKa = 11.84KK831 pKa = 9.19LRR833 pKa = 11.84NLEE836 pKa = 3.83NQGIYY841 pKa = 10.22VDD843 pKa = 3.85EE844 pKa = 4.46SKK846 pKa = 10.9IMSRR850 pKa = 11.84GRR852 pKa = 11.84ISKK855 pKa = 10.31EE856 pKa = 3.55DD857 pKa = 3.35TEE859 pKa = 4.37LAMRR863 pKa = 11.84IARR866 pKa = 11.84KK867 pKa = 7.61NQKK870 pKa = 9.05DD871 pKa = 3.23AKK873 pKa = 10.2LRR875 pKa = 11.84RR876 pKa = 11.84IYY878 pKa = 11.04SNNASIQEE886 pKa = 4.45SYY888 pKa = 10.35TVDD891 pKa = 3.8DD892 pKa = 4.99FVSYY896 pKa = 10.15WMEE899 pKa = 4.1QEE901 pKa = 4.19SLPTGIQIAMWLKK914 pKa = 10.72GDD916 pKa = 4.66DD917 pKa = 3.72WSQPIPPRR925 pKa = 11.84VQRR928 pKa = 11.84RR929 pKa = 11.84HH930 pKa = 5.12YY931 pKa = 10.67DD932 pKa = 3.13SYY934 pKa = 11.93IMMLGPSPTQEE945 pKa = 3.57QADD948 pKa = 3.94AVKK951 pKa = 10.9DD952 pKa = 3.99LVDD955 pKa = 5.6DD956 pKa = 4.59IYY958 pKa = 11.71DD959 pKa = 3.79RR960 pKa = 11.84NQGKK964 pKa = 9.79GPSQEE969 pKa = 3.94QARR972 pKa = 11.84EE973 pKa = 3.6LSHH976 pKa = 6.56AVRR979 pKa = 11.84RR980 pKa = 11.84LISHH984 pKa = 6.36SLVNQPATAPRR995 pKa = 11.84VPPRR999 pKa = 11.84RR1000 pKa = 11.84IVSAQTAQTDD1010 pKa = 3.89PPGRR1014 pKa = 11.84RR1015 pKa = 11.84AALDD1019 pKa = 3.04RR1020 pKa = 11.84LRR1022 pKa = 11.84RR1023 pKa = 11.84VRR1025 pKa = 11.84GEE1027 pKa = 4.23DD1028 pKa = 3.12NDD1030 pKa = 3.87IVV1032 pKa = 3.53

Molecular weight: 114.03 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|Q91CD5|RDRP_DXV96 RNA-directed RNA polymerase OS=Drosophila x virus (isolate Chung/1996) OX=654931 GN=VP1 PE=3 SV=2
MM1 pKa = 7.44LSIIRR6 pKa = 11.84RR7 pKa = 11.84KK8 pKa = 7.68TRR10 pKa = 11.84IVDD13 pKa = 3.05ITKK16 pKa = 10.21QGNGNVPPACHH27 pKa = 6.01LCSRR31 pKa = 11.84RR32 pKa = 11.84LTKK35 pKa = 9.88EE36 pKa = 3.27WSFGEE41 pKa = 4.05GTICSFHH48 pKa = 7.63RR49 pKa = 11.84IRR51 pKa = 11.84CRR53 pKa = 11.84VQCGRR58 pKa = 11.84SFWHH62 pKa = 6.17QPEE65 pKa = 4.26TPIDD69 pKa = 3.95GLSWDD74 pKa = 3.71STTWEE79 pKa = 4.11LTRR82 pKa = 11.84ISSSKK87 pKa = 11.04DD88 pKa = 3.02PDD90 pKa = 3.12ISGQKK95 pKa = 9.53DD96 pKa = 2.87KK97 pKa = 11.13RR98 pKa = 11.84YY99 pKa = 10.13GRR101 pKa = 11.84RR102 pKa = 11.84KK103 pKa = 9.38EE104 pKa = 4.22KK105 pKa = 10.68NPKK108 pKa = 8.49TDD110 pKa = 3.52PPALDD115 pKa = 3.27SRR117 pKa = 11.84VRR119 pKa = 11.84EE120 pKa = 3.98HH121 pKa = 6.9EE122 pKa = 4.75PIHH125 pKa = 5.07EE126 pKa = 4.48HH127 pKa = 5.86EE128 pKa = 5.36PIPGRR133 pKa = 11.84VGPADD138 pKa = 3.28TKK140 pKa = 10.48QRR142 pKa = 11.84CKK144 pKa = 11.15ANLRR148 pKa = 11.84GDD150 pKa = 3.65SGFVSIGRR158 pKa = 11.84SNHH161 pKa = 5.93PKK163 pKa = 10.47LSRR166 pKa = 11.84EE167 pKa = 4.08DD168 pKa = 3.56CHH170 pKa = 5.68NTRR173 pKa = 11.84VPPGTQGVRR182 pKa = 11.84GGNVQLDD189 pKa = 4.03KK190 pKa = 10.98PRR192 pKa = 11.84EE193 pKa = 3.97RR194 pKa = 11.84PVSYY198 pKa = 10.21QHH200 pKa = 6.48GSKK203 pKa = 10.11KK204 pKa = 10.17RR205 pKa = 11.84SEE207 pKa = 4.11HH208 pKa = 6.25RR209 pKa = 11.84NPVSRR214 pKa = 11.84SHH216 pKa = 6.41RR217 pKa = 11.84RR218 pKa = 11.84KK219 pKa = 9.46KK220 pKa = 10.73AKK222 pKa = 9.43TRR224 pKa = 11.84TKK226 pKa = 9.67TSKK229 pKa = 10.24LGKK232 pKa = 10.21SRR234 pKa = 11.84DD235 pKa = 3.24ICC237 pKa = 4.78

Molecular weight: 27.06 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

2266

237

1032

755.3

84.62

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.781 ± 0.786

0.574 ± 0.594

5.384 ± 0.149

5.56 ± 0.531

2.295 ± 0.153

6.311 ± 0.597

1.986 ± 0.594

6.134 ± 0.305

6.002 ± 1.237

8.738 ± 0.994

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.692 ± 0.485

5.869 ± 0.659

6.134 ± 0.31

4.016 ± 0.203

5.605 ± 1.601

8.914 ± 0.769

6.399 ± 0.18

6.046 ± 0.3

1.412 ± 0.138

4.148 ± 0.794
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski