Rhodopseudomonas palustris

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhizobiales; Bradyrhizobiaceae; Rhodopseudomonas

Average proteome isoelectric point is 6.76

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5580 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0D7EU90|A0A0D7EU90_RHOPL Photosystem reaction center subunit H OS=Rhodopseudomonas palustris OX=1076 GN=OO17_09930 PE=4 SV=1
MM1 pKa = 7.34TISVTEE7 pKa = 4.8AIEE10 pKa = 3.99QNTKK14 pKa = 10.28GMSDD18 pKa = 4.01GLPVGVPASYY28 pKa = 10.19SWYY31 pKa = 8.55TGAVSDD37 pKa = 5.12SDD39 pKa = 3.77IPPADD44 pKa = 3.99FSSVGGWGQIYY55 pKa = 9.81PEE57 pKa = 4.19QGSSYY62 pKa = 10.45SNPNATVEE70 pKa = 4.25VANAQTWVHH79 pKa = 6.78LSTTNQWVLVQSQANDD95 pKa = 3.9PLEE98 pKa = 4.62GGHH101 pKa = 6.17FVADD105 pKa = 4.24FAGNAGIGMEE115 pKa = 4.3VTQGADD121 pKa = 3.09GTATMEE127 pKa = 4.28VPPLGYY133 pKa = 9.85NDD135 pKa = 4.75HH136 pKa = 6.66FWHH139 pKa = 6.53VGRR142 pKa = 11.84GQFAPGTVDD151 pKa = 3.25GVYY154 pKa = 10.5VQMDD158 pKa = 3.81MKK160 pKa = 10.84ISDD163 pKa = 4.28PNVKK167 pKa = 10.05AVANIGADD175 pKa = 2.9WWRR178 pKa = 11.84TPTADD183 pKa = 3.63YY184 pKa = 11.59VDD186 pKa = 4.22GFGNNPGAGMSNWVEE201 pKa = 3.84LTGDD205 pKa = 3.7YY206 pKa = 8.0QTLAFYY212 pKa = 10.78SGTAAEE218 pKa = 5.06FRR220 pKa = 11.84ADD222 pKa = 4.28PPPCCTDD229 pKa = 4.99AIITNPNPPVTDD241 pKa = 3.79PPGGIGTDD249 pKa = 3.63TPIVIPPGSTPDD261 pKa = 3.47PVVGVNLLKK270 pKa = 10.95NGSFEE275 pKa = 4.11ATPVGSGQAGIQTQINGWTALSGSAIEE302 pKa = 4.38LWNNLGGVQATEE314 pKa = 3.99GNNFVEE320 pKa = 4.52LDD322 pKa = 3.49YY323 pKa = 11.23MGAQDD328 pKa = 5.07GFSQSVQTSSGQALTLSVDD347 pKa = 2.66ARR349 pKa = 11.84TRR351 pKa = 11.84PGEE354 pKa = 3.91NPYY357 pKa = 10.61SCGVEE362 pKa = 3.98VLWNGEE368 pKa = 4.41VISTIQPGADD378 pKa = 2.43WSTYY382 pKa = 8.17SVNVTGTGGNDD393 pKa = 3.0TLTIRR398 pKa = 11.84EE399 pKa = 4.26PQNQAFDD406 pKa = 3.99GVGAMLDD413 pKa = 3.85NLKK416 pKa = 10.45LAANTASTPTDD427 pKa = 3.53DD428 pKa = 4.2TDD430 pKa = 5.28LPSTDD435 pKa = 3.85PTPGGSGTDD444 pKa = 3.14

Molecular weight: 46.2 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0D7E3J2|A0A0D7E3J2_RHOPL Uncharacterized protein OS=Rhodopseudomonas palustris OX=1076 GN=OO17_25755 PE=4 SV=1
MM1 pKa = 6.88TTFLSSILLPVLVGAVTLVLLLGLINMMRR30 pKa = 11.84GGSPNRR36 pKa = 11.84SQTLMRR42 pKa = 11.84WRR44 pKa = 11.84VLLQFAAIIVTMLAVWAMGRR64 pKa = 3.49

Molecular weight: 7.04 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5580

0

5580

1615923

26

3315

289.6

31.35

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.881 ± 0.043

0.863 ± 0.011

5.74 ± 0.025

5.103 ± 0.03

3.712 ± 0.02

8.395 ± 0.028

2.016 ± 0.016

5.471 ± 0.023

3.481 ± 0.034

10.078 ± 0.044

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.491 ± 0.015

2.627 ± 0.019

5.247 ± 0.03

3.283 ± 0.02

6.936 ± 0.036

5.501 ± 0.023

5.261 ± 0.02

7.416 ± 0.024

1.296 ± 0.013

2.202 ± 0.016
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski