Escherichia phage vB_EcoS-IME253

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Drexlerviridae; Braunvirinae; Rtpvirus; Escherichia virus IME253

Average proteome isoelectric point is 6.17

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 74 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1P8DUL7|A0A1P8DUL7_9CAUD Uncharacterized protein OS=Escherichia phage vB_EcoS-IME253 OX=1933412 PE=4 SV=1
MM1 pKa = 8.16IGICVKK7 pKa = 10.14TNGFFTLLEE16 pKa = 4.6DD17 pKa = 3.83YY18 pKa = 10.34EE19 pKa = 4.99INFDD23 pKa = 3.99SNEE26 pKa = 3.93ITVLSDD32 pKa = 3.69DD33 pKa = 3.4GCYY36 pKa = 9.28WFLSYY41 pKa = 10.73QGDD44 pKa = 4.13GYY46 pKa = 11.01YY47 pKa = 10.84GCRR50 pKa = 11.84DD51 pKa = 3.33SGGKK55 pKa = 8.82EE56 pKa = 3.52VSFLVEE62 pKa = 3.75

Molecular weight: 7.03 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1P8DUU2|A0A1P8DUU2_9CAUD Uncharacterized protein OS=Escherichia phage vB_EcoS-IME253 OX=1933412 PE=4 SV=1
MM1 pKa = 6.8NTVDD5 pKa = 4.7KK6 pKa = 10.59RR7 pKa = 11.84TINGNNGTVRR17 pKa = 11.84TEE19 pKa = 4.03DD20 pKa = 2.79KK21 pKa = 10.45KK22 pKa = 10.46QRR24 pKa = 11.84KK25 pKa = 8.53RR26 pKa = 11.84PSGYY30 pKa = 10.06YY31 pKa = 8.95VLKK34 pKa = 10.9DD35 pKa = 3.51EE36 pKa = 4.52VRR38 pKa = 11.84AGLRR42 pKa = 11.84ARR44 pKa = 11.84LEE46 pKa = 3.84IVLDD50 pKa = 3.88FFGTKK55 pKa = 10.47ANIAKK60 pKa = 9.21QLKK63 pKa = 7.58VTPQAVEE70 pKa = 3.72EE71 pKa = 4.24WFKK74 pKa = 11.38RR75 pKa = 11.84GMISARR81 pKa = 11.84GAQLSHH87 pKa = 6.72NYY89 pKa = 9.66YY90 pKa = 10.0KK91 pKa = 10.9RR92 pKa = 11.84NGEE95 pKa = 3.9GFRR98 pKa = 11.84ATFCRR103 pKa = 11.84PDD105 pKa = 3.38LQFDD109 pKa = 4.36GNGKK113 pKa = 8.92PLTLRR118 pKa = 11.84CKK120 pKa = 9.49KK121 pKa = 9.98RR122 pKa = 11.84HH123 pKa = 4.86MLRR126 pKa = 11.84VVTEE130 pKa = 4.32SEE132 pKa = 4.17LATKK136 pKa = 9.22PEE138 pKa = 3.98CRR140 pKa = 11.84SWRR143 pKa = 11.84KK144 pKa = 9.4IKK146 pKa = 10.36AANEE150 pKa = 3.74AARR153 pKa = 11.84KK154 pKa = 9.56AKK156 pKa = 9.88EE157 pKa = 3.61

Molecular weight: 18.06 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

74

0

74

13985

41

1105

189.0

21.06

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.252 ± 0.517

1.251 ± 0.161

6.064 ± 0.266

6.679 ± 0.369

3.883 ± 0.181

7.336 ± 0.262

1.387 ± 0.179

6.85 ± 0.197

6.736 ± 0.446

7.136 ± 0.257

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.567 ± 0.243

5.32 ± 0.288

2.946 ± 0.229

3.74 ± 0.415

4.884 ± 0.191

7.086 ± 0.495

5.492 ± 0.283

7.236 ± 0.306

1.258 ± 0.106

3.897 ± 0.241
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski