Flavobacterium fluvii

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Flavobacteriia; Flavobacteriales; Flavobacteriaceae; Flavobacterium

Average proteome isoelectric point is 6.67

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3488 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1M5HC98|A0A1M5HC98_9FLAO PKD domain-containing protein OS=Flavobacterium fluvii OX=468056 GN=SAMN05443549_102174 PE=4 SV=1
MM1 pKa = 7.59NLTQEE6 pKa = 4.5DD7 pKa = 4.24WVSQYY12 pKa = 10.9EE13 pKa = 3.98ADD15 pKa = 4.1DD16 pKa = 3.73NAVILDD22 pKa = 3.55VRR24 pKa = 11.84TEE26 pKa = 4.09AEE28 pKa = 4.33VNEE31 pKa = 4.01GMIPNAVNIDD41 pKa = 3.39IYY43 pKa = 10.63EE44 pKa = 4.25GQGFIDD50 pKa = 4.97KK51 pKa = 10.47LAALDD56 pKa = 3.66KK57 pKa = 10.87SKK59 pKa = 11.2NYY61 pKa = 10.3YY62 pKa = 9.34VYY64 pKa = 10.0CRR66 pKa = 11.84SGARR70 pKa = 11.84SAKK73 pKa = 10.36ACDD76 pKa = 3.51VMNEE80 pKa = 4.06LGFEE84 pKa = 3.99NAYY87 pKa = 10.79NLLGGFLEE95 pKa = 4.36WDD97 pKa = 3.8GEE99 pKa = 4.83VVV101 pKa = 3.25

Molecular weight: 11.31 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1M5IY58|A0A1M5IY58_9FLAO Uncharacterized conserved protein YndB AHSA1/START domain OS=Flavobacterium fluvii OX=468056 GN=SAMN05443549_103276 PE=3 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.01RR4 pKa = 11.84TFQPSKK10 pKa = 9.13RR11 pKa = 11.84KK12 pKa = 9.48RR13 pKa = 11.84RR14 pKa = 11.84NKK16 pKa = 9.91HH17 pKa = 4.39GFMDD21 pKa = 4.5RR22 pKa = 11.84MASANGRR29 pKa = 11.84KK30 pKa = 9.04VLARR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84AKK38 pKa = 10.19GRR40 pKa = 11.84HH41 pKa = 5.41KK42 pKa = 10.14LTVSSEE48 pKa = 3.92PRR50 pKa = 11.84HH51 pKa = 5.77KK52 pKa = 10.61KK53 pKa = 9.84

Molecular weight: 6.3 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3488

0

3488

1237016

29

5891

354.6

39.87

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.549 ± 0.037

0.797 ± 0.016

5.312 ± 0.027

6.195 ± 0.053

5.263 ± 0.039

6.587 ± 0.041

1.624 ± 0.018

7.953 ± 0.041

8.065 ± 0.058

8.943 ± 0.046

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.226 ± 0.021

6.396 ± 0.043

3.528 ± 0.026

3.318 ± 0.021

3.067 ± 0.027

6.56 ± 0.037

6.083 ± 0.076

6.331 ± 0.03

1.152 ± 0.018

4.052 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski