Amylibacter kogurei

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales; Rhodobacteraceae; Amylibacter

Average proteome isoelectric point is 6.17

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2837 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2G5K4C5|A0A2G5K4C5_9RHOB Diguanylate cyclase OS=Amylibacter kogurei OX=1889778 GN=BFP76_01515 PE=3 SV=1
MM1 pKa = 8.06VLITNFGEE9 pKa = 4.38VTGDD13 pKa = 2.59ISMGFFSIFRR23 pKa = 11.84YY24 pKa = 9.77SSHH27 pKa = 6.23NSYY30 pKa = 8.8STRR33 pKa = 11.84NWWDD37 pKa = 3.0RR38 pKa = 11.84DD39 pKa = 3.69SRR41 pKa = 11.84DD42 pKa = 4.58DD43 pKa = 5.56RR44 pKa = 11.84DD45 pKa = 3.49DD46 pKa = 4.49CRR48 pKa = 11.84DD49 pKa = 3.3GDD51 pKa = 3.8RR52 pKa = 11.84NSYY55 pKa = 9.96DD56 pKa = 2.78RR57 pKa = 11.84HH58 pKa = 5.9DD59 pKa = 3.98RR60 pKa = 11.84YY61 pKa = 10.82DD62 pKa = 3.05RR63 pKa = 11.84HH64 pKa = 6.64DD65 pKa = 3.9RR66 pKa = 11.84YY67 pKa = 11.3DD68 pKa = 4.02RR69 pKa = 11.84DD70 pKa = 3.85DD71 pKa = 4.68RR72 pKa = 11.84DD73 pKa = 3.22WWSHH77 pKa = 7.15DD78 pKa = 3.7NDD80 pKa = 3.99DD81 pKa = 4.62CQTSGSRR88 pKa = 11.84YY89 pKa = 10.33DD90 pKa = 3.43NCNDD94 pKa = 5.26DD95 pKa = 5.98RR96 pKa = 11.84DD97 pKa = 4.21DD98 pKa = 5.14DD99 pKa = 4.68CDD101 pKa = 3.7NTSWWHH107 pKa = 6.11SHH109 pKa = 7.66DD110 pKa = 5.32DD111 pKa = 3.68DD112 pKa = 6.55CNDD115 pKa = 5.11DD116 pKa = 4.13RR117 pKa = 11.84DD118 pKa = 4.02NDD120 pKa = 3.73DD121 pKa = 5.89RR122 pKa = 11.84NDD124 pKa = 3.62NDD126 pKa = 4.77CDD128 pKa = 4.26DD129 pKa = 5.14DD130 pKa = 4.24RR131 pKa = 11.84WGHH134 pKa = 4.67NHH136 pKa = 7.18DD137 pKa = 4.56WNCDD141 pKa = 3.46DD142 pKa = 5.05DD143 pKa = 5.22TVDD146 pKa = 5.91NDD148 pKa = 4.53DD149 pKa = 5.5HH150 pKa = 7.76DD151 pKa = 6.68DD152 pKa = 3.94NDD154 pKa = 5.12DD155 pKa = 4.29DD156 pKa = 6.02QGDD159 pKa = 4.68DD160 pKa = 4.87DD161 pKa = 5.55DD162 pKa = 6.3QGDD165 pKa = 4.33DD166 pKa = 4.08LCDD169 pKa = 3.77GTDD172 pKa = 3.3EE173 pKa = 5.06DD174 pKa = 5.18GNPICEE180 pKa = 4.25EE181 pKa = 4.01EE182 pKa = 4.19EE183 pKa = 4.49EE184 pKa = 4.52EE185 pKa = 5.33VDD187 pKa = 3.66SCDD190 pKa = 3.99YY191 pKa = 11.23DD192 pKa = 4.23VSDD195 pKa = 4.05WFCLPSHH202 pKa = 7.23HH203 pKa = 6.88YY204 pKa = 9.18NHH206 pKa = 7.02SYY208 pKa = 11.32DD209 pKa = 5.53CDD211 pKa = 4.77DD212 pKa = 4.67EE213 pKa = 7.48DD214 pKa = 5.78DD215 pKa = 5.44DD216 pKa = 4.86EE217 pKa = 7.78DD218 pKa = 4.35IVEE221 pKa = 4.1VDD223 pKa = 3.07

Molecular weight: 26.67 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2G5K5E4|A0A2G5K5E4_9RHOB DUF4375 domain-containing protein OS=Amylibacter kogurei OX=1889778 GN=BFP76_03195 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.44RR3 pKa = 11.84TYY5 pKa = 10.31QPSNLVRR12 pKa = 11.84KK13 pKa = 8.96RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.62GFRR19 pKa = 11.84SRR21 pKa = 11.84MATKK25 pKa = 10.45NGRR28 pKa = 11.84KK29 pKa = 8.78ILNRR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AQGRR39 pKa = 11.84KK40 pKa = 9.3SLSAA44 pKa = 3.83

Molecular weight: 5.27 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2837

0

2837

911759

34

3452

321.4

35.19

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.61 ± 0.047

0.966 ± 0.015

6.38 ± 0.062

5.51 ± 0.047

4.173 ± 0.031

8.051 ± 0.05

2.108 ± 0.022

6.352 ± 0.039

4.472 ± 0.039

9.346 ± 0.052

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.97 ± 0.023

3.775 ± 0.026

4.424 ± 0.032

3.504 ± 0.027

5.393 ± 0.041

5.625 ± 0.03

5.622 ± 0.048

6.952 ± 0.034

1.301 ± 0.018

2.467 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski