Gordonia phage Stultus

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Vividuovirus; unclassified Vividuovirus

Average proteome isoelectric point is 5.83

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 80 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3G3MBH8|A0A3G3MBH8_9CAUD Helix-turn-helix DNA binding protein OS=Gordonia phage Stultus OX=2483674 GN=79 PE=4 SV=1
MM1 pKa = 7.6ASDD4 pKa = 5.73DD5 pKa = 3.67IRR7 pKa = 11.84NYY9 pKa = 11.28DD10 pKa = 3.81PDD12 pKa = 3.28TGMLYY17 pKa = 9.88RR18 pKa = 11.84WEE20 pKa = 4.57MPDD23 pKa = 3.1SPRR26 pKa = 11.84GFVVEE31 pKa = 5.22IEE33 pKa = 4.35NDD35 pKa = 3.4DD36 pKa = 5.08DD37 pKa = 5.74GPILCGCVSGPGFTKK52 pKa = 10.43PAGFLYY58 pKa = 9.89PAEE61 pKa = 4.29ARR63 pKa = 11.84WLAEE67 pKa = 3.93NMPSMLASIEE77 pKa = 4.22AYY79 pKa = 9.66EE80 pKa = 4.16LQQGATSS87 pKa = 3.39

Molecular weight: 9.58 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3G3MBQ3|A0A3G3MBQ3_9CAUD Uncharacterized protein OS=Gordonia phage Stultus OX=2483674 GN=28 PE=4 SV=1
MM1 pKa = 7.26GWLLFGAVFIPVRR14 pKa = 11.84IAKK17 pKa = 9.9IIEE20 pKa = 4.48DD21 pKa = 3.61ALSPRR26 pKa = 11.84RR27 pKa = 11.84RR28 pKa = 11.84RR29 pKa = 11.84EE30 pKa = 3.9LIQQQTRR37 pKa = 11.84DD38 pKa = 3.68EE39 pKa = 4.24VAAAAEE45 pKa = 4.0RR46 pKa = 11.84SEE48 pKa = 4.39QYY50 pKa = 10.66RR51 pKa = 11.84RR52 pKa = 11.84THH54 pKa = 6.07YY55 pKa = 11.12GLL57 pKa = 4.1

Molecular weight: 6.68 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

80

0

80

18469

37

1607

230.9

25.03

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.356 ± 0.416

0.715 ± 0.106

7.467 ± 0.43

5.582 ± 0.394

2.453 ± 0.154

8.549 ± 0.471

2.09 ± 0.191

4.516 ± 0.249

2.529 ± 0.174

7.58 ± 0.234

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.117 ± 0.117

2.675 ± 0.152

6.438 ± 0.268

3.844 ± 0.199

7.207 ± 0.425

4.873 ± 0.218

6.725 ± 0.354

7.992 ± 0.242

2.101 ± 0.121

2.193 ± 0.13
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski