Fibrobacter succinogenes (strain ATCC 19169 / S85)

Taxonomy: cellular organisms; Bacteria; FCB group; Fibrobacteres; Fibrobacteria; Fibrobacterales; Fibrobacteraceae; Fibrobacter; Fibrobacter succinogenes; Fibrobacter succinogenes subsp. succinogenes

Average proteome isoelectric point is 6.34

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2871 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|C9RN46|C9RN46_FIBSS Acetolactate synthase OS=Fibrobacter succinogenes (strain ATCC 19169 / S85) OX=59374 GN=ilvB PE=3 SV=1
MM1 pKa = 8.19DD2 pKa = 4.25CRR4 pKa = 11.84ILVRR8 pKa = 11.84FIGVQEE14 pKa = 4.75DD15 pKa = 4.99LDD17 pKa = 4.31ALWSAFMEE25 pKa = 4.39HH26 pKa = 6.38FPEE29 pKa = 6.13AEE31 pKa = 4.14LPSVDD36 pKa = 3.16SGEE39 pKa = 4.18WYY41 pKa = 10.53SVEE44 pKa = 5.78DD45 pKa = 3.89YY46 pKa = 7.74MTDD49 pKa = 3.03EE50 pKa = 4.35YY51 pKa = 11.31EE52 pKa = 4.34CRR54 pKa = 11.84FEE56 pKa = 4.37VLPEE60 pKa = 4.04SEE62 pKa = 5.39LYY64 pKa = 10.83AIDD67 pKa = 3.57GTFLVEE73 pKa = 4.31NEE75 pKa = 4.19PPEE78 pKa = 4.76DD79 pKa = 3.58VLTEE83 pKa = 3.54ILEE86 pKa = 4.24RR87 pKa = 11.84FEE89 pKa = 4.26RR90 pKa = 11.84VYY92 pKa = 11.32AVFKK96 pKa = 10.12WSTMDD101 pKa = 3.27VGVGCGTSEE110 pKa = 3.79GWGEE114 pKa = 4.13FNADD118 pKa = 3.92RR119 pKa = 11.84ADD121 pKa = 4.36DD122 pKa = 4.83GSDD125 pKa = 3.19EE126 pKa = 4.27ANEE129 pKa = 3.73ISEE132 pKa = 4.38AVLGFF137 pKa = 4.02

Molecular weight: 15.62 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|D9S563|D9S563_FIBSS Hydrolase carbon-nitrogen family OS=Fibrobacter succinogenes (strain ATCC 19169 / S85) OX=59374 GN=FSU_0240 PE=4 SV=1
MM1 pKa = 8.07DD2 pKa = 4.96GKK4 pKa = 10.96LVLALSRR11 pKa = 11.84ASRR14 pKa = 11.84HH15 pKa = 4.57WSTGVTVGASAAKK28 pKa = 10.39LMEE31 pKa = 4.44AARR34 pKa = 11.84APRR37 pKa = 11.84VKK39 pKa = 10.23ILIVFIFAAPYY50 pKa = 9.61FLVSFSRR57 pKa = 11.84MLWYY61 pKa = 8.17RR62 pKa = 11.84TLRR65 pKa = 11.84SSSARR70 pKa = 11.84RR71 pKa = 11.84DD72 pKa = 3.29SSSFLSSFLAFSFSDD87 pKa = 3.26LAMASRR93 pKa = 11.84ISSSLCSASAWASSIFPSSFRR114 pKa = 11.84ALAATSLEE122 pKa = 4.17YY123 pKa = 10.91EE124 pKa = 4.28FDD126 pKa = 3.68VMLASSLFSLTSEE139 pKa = 4.5RR140 pKa = 11.84VASACPRR147 pKa = 11.84EE148 pKa = 4.01RR149 pKa = 11.84LIPLWLPEE157 pKa = 4.04QPVRR161 pKa = 11.84AARR164 pKa = 11.84ASVKK168 pKa = 9.78EE169 pKa = 3.94HH170 pKa = 6.44ANILAFIGASPMIDD184 pKa = 2.86IPRR187 pKa = 3.99

Molecular weight: 20.45 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2871

0

2871

1073633

30

3318

374.0

41.78

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.109 ± 0.051

1.4 ± 0.021

5.994 ± 0.037

6.551 ± 0.044

4.883 ± 0.035

6.951 ± 0.05

1.786 ± 0.017

6.14 ± 0.042

6.961 ± 0.037

8.716 ± 0.051

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.601 ± 0.024

4.829 ± 0.036

3.799 ± 0.027

2.882 ± 0.024

4.462 ± 0.036

6.733 ± 0.053

5.236 ± 0.037

6.97 ± 0.043

1.282 ± 0.019

3.715 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski