Actinobacteria bacterium IMCC26256

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; unclassified Actinomycetia

Average proteome isoelectric point is 5.98

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2299 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0G3V3F2|A0A0G3V3F2_9ACTN Uncharacterized protein OS=Actinobacteria bacterium IMCC26256 OX=1650658 GN=IMCC26256_111166 PE=4 SV=1
MM1 pKa = 7.4TGATGATGADD11 pKa = 3.62GSNGSDD17 pKa = 3.13GAAGANGSDD26 pKa = 3.63GATGVTGEE34 pKa = 4.1QGIPGTATMTGATGATGADD53 pKa = 3.58GSNGSNGADD62 pKa = 2.89GVTGADD68 pKa = 3.75GSNGSNGATGANGSNGSNGADD89 pKa = 3.5GATGAAGPAGPQGVTGATGATGADD113 pKa = 3.58GSNGSNGATGADD125 pKa = 3.92GATGAAGPAGPQGVTGATGATGSDD149 pKa = 3.46GTNGIDD155 pKa = 5.29GINGSNGADD164 pKa = 3.18GSNGSNGVDD173 pKa = 3.41GAAGANGSNGSNGVDD188 pKa = 3.28GATGATGATGATGVTGTNGTNGAAGATGAAGPAGPQGVTGATGATGATGTNGTNGSTGAAGPAGPQGLTGATGATGATGTNGTNGAVGAAGPAGPQGVAGATGAGYY294 pKa = 10.79AGVTSATSLTIGTGSKK310 pKa = 9.25TFTITAGSAFVTGARR325 pKa = 11.84VRR327 pKa = 11.84AANTTTPANYY337 pKa = 9.07MEE339 pKa = 5.37GIVTVSGTTLTMTSDD354 pKa = 3.45AVGGSGTFAAWTFSIVGNVGATGAAGVNGSNGANGANGTNGSTGATGATGVASVYY409 pKa = 10.8SGATGLTMTTPKK421 pKa = 10.1IVAGYY426 pKa = 9.45VAVGSNDD433 pKa = 2.79FTTISLTNGSAFTSATSYY451 pKa = 11.01FCTATEE457 pKa = 3.81ASANNSTAAITKK469 pKa = 9.17VSIFRR474 pKa = 11.84TSGASFSINVEE485 pKa = 4.13SNKK488 pKa = 10.12TITGVMYY495 pKa = 9.43TCIGNN500 pKa = 3.49

Molecular weight: 44.13 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0G3UZZ2|A0A0G3UZZ2_9ACTN 4HBT domain-containing protein OS=Actinobacteria bacterium IMCC26256 OX=1650658 GN=IMCC26256_1139 PE=4 SV=1
MM1 pKa = 7.72PPAKK5 pKa = 9.49KK6 pKa = 7.01TTRR9 pKa = 11.84KK10 pKa = 9.58AAAKK14 pKa = 9.21KK15 pKa = 10.51APAKK19 pKa = 10.31RR20 pKa = 11.84KK21 pKa = 8.88AAKK24 pKa = 9.53KK25 pKa = 10.41APAKK29 pKa = 10.22RR30 pKa = 11.84KK31 pKa = 9.14AAAKK35 pKa = 9.26KK36 pKa = 10.42APAKK40 pKa = 10.31RR41 pKa = 11.84KK42 pKa = 8.88AAKK45 pKa = 9.53KK46 pKa = 10.41APAKK50 pKa = 10.22RR51 pKa = 11.84KK52 pKa = 9.14AAAKK56 pKa = 9.26KK57 pKa = 10.42APAKK61 pKa = 10.31RR62 pKa = 11.84KK63 pKa = 8.88AAKK66 pKa = 9.53KK67 pKa = 10.41APAKK71 pKa = 10.31RR72 pKa = 11.84KK73 pKa = 8.73AAKK76 pKa = 9.73KK77 pKa = 9.34AAKK80 pKa = 9.39KK81 pKa = 10.61APAKK85 pKa = 10.31RR86 pKa = 11.84KK87 pKa = 8.88AAKK90 pKa = 9.53KK91 pKa = 10.41APAKK95 pKa = 10.31RR96 pKa = 11.84KK97 pKa = 8.88AAKK100 pKa = 9.53KK101 pKa = 10.41APAKK105 pKa = 10.31RR106 pKa = 11.84KK107 pKa = 8.73AAKK110 pKa = 9.73KK111 pKa = 9.34AAKK114 pKa = 9.39KK115 pKa = 10.61APAKK119 pKa = 10.31RR120 pKa = 11.84KK121 pKa = 8.88AAKK124 pKa = 9.53KK125 pKa = 10.41APAKK129 pKa = 10.31RR130 pKa = 11.84KK131 pKa = 8.88AAKK134 pKa = 9.53KK135 pKa = 10.41APAKK139 pKa = 10.31RR140 pKa = 11.84KK141 pKa = 8.68AAKK144 pKa = 9.72KK145 pKa = 7.87ATRR148 pKa = 11.84RR149 pKa = 11.84KK150 pKa = 9.87

Molecular weight: 15.78 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2299

0

2299

757619

15

1532

329.5

35.49

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.66 ± 0.056

0.891 ± 0.015

5.764 ± 0.043

6.282 ± 0.061

3.31 ± 0.028

8.862 ± 0.057

1.932 ± 0.023

5.428 ± 0.036

2.729 ± 0.034

9.839 ± 0.047

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.034 ± 0.022

2.626 ± 0.028

5.086 ± 0.029

2.567 ± 0.024

6.894 ± 0.048

6.729 ± 0.046

5.687 ± 0.05

8.276 ± 0.046

1.397 ± 0.02

2.008 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski