Riemerella columbipharyngis

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Flavobacteriia; Flavobacteriales; Weeksellaceae; Riemerella

Average proteome isoelectric point is 7.0

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2118 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1G6ZJS8|A0A1G6ZJS8_9FLAO Uncharacterized protein OS=Riemerella columbipharyngis OX=1071918 GN=SAMN05421544_102110 PE=3 SV=1
MM1 pKa = 7.78GDD3 pKa = 3.47VNIKK7 pKa = 8.6ITDD10 pKa = 3.81RR11 pKa = 11.84NGVTHH16 pKa = 6.56NVAAPTDD23 pKa = 3.69MAMSLMEE30 pKa = 3.87VVRR33 pKa = 11.84AYY35 pKa = 10.1EE36 pKa = 3.79IAEE39 pKa = 4.48DD40 pKa = 4.04GTIGVCGGMAMCASCQVYY58 pKa = 10.1VLNDD62 pKa = 3.48TPLPEE67 pKa = 4.73MGDD70 pKa = 3.61EE71 pKa = 4.09EE72 pKa = 4.99DD73 pKa = 4.83AMLAEE78 pKa = 4.71AFHH81 pKa = 6.12VQDD84 pKa = 4.19NSRR87 pKa = 11.84LGCQIPITEE96 pKa = 4.21EE97 pKa = 4.15LEE99 pKa = 4.11GLEE102 pKa = 4.37VQLAPYY108 pKa = 9.06PP109 pKa = 3.86

Molecular weight: 11.73 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1G6Y5G3|A0A1G6Y5G3_9FLAO MFS transporter FHS family L-fucose permease OS=Riemerella columbipharyngis OX=1071918 GN=SAMN05421544_10135 PE=4 SV=1
MM1 pKa = 7.84PSGKK5 pKa = 9.32KK6 pKa = 9.59RR7 pKa = 11.84KK8 pKa = 7.05RR9 pKa = 11.84HH10 pKa = 5.11KK11 pKa = 10.59VATHH15 pKa = 5.21KK16 pKa = 10.25RR17 pKa = 11.84KK18 pKa = 9.66KK19 pKa = 9.26RR20 pKa = 11.84RR21 pKa = 11.84RR22 pKa = 11.84LNRR25 pKa = 11.84HH26 pKa = 5.27KK27 pKa = 10.6KK28 pKa = 9.72KK29 pKa = 10.58KK30 pKa = 10.03

Molecular weight: 3.8 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2118

0

2118

624644

27

2383

294.9

33.51

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.186 ± 0.056

0.792 ± 0.017

5.436 ± 0.04

6.894 ± 0.065

5.111 ± 0.047

6.258 ± 0.053

1.715 ± 0.023

8.054 ± 0.053

8.823 ± 0.059

9.021 ± 0.05

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.429 ± 0.024

6.021 ± 0.056

3.344 ± 0.033

3.519 ± 0.037

3.803 ± 0.036

6.243 ± 0.042

5.117 ± 0.036

5.924 ± 0.036

1.004 ± 0.019

4.305 ± 0.045
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski