Mycoplasma testudineum

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Tenericutes; Mollicutes; Mycoplasmatales; Mycoplasmataceae; Mycoplasma

Average proteome isoelectric point is 7.33

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 783 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4R6ICR4|A0A4R6ICR4_9MOLU Uncharacterized protein OS=Mycoplasma testudineum OX=244584 GN=EI74_0575 PE=4 SV=1
MM1 pKa = 7.4NIEE4 pKa = 4.49KK5 pKa = 10.15EE6 pKa = 3.98IEE8 pKa = 3.94DD9 pKa = 3.29RR10 pKa = 11.84SMVFEE15 pKa = 5.7DD16 pKa = 3.4STEE19 pKa = 4.51GIGLLIIPEE28 pKa = 4.17EE29 pKa = 4.27DD30 pKa = 3.27EE31 pKa = 4.21EE32 pKa = 5.92LIFVYY37 pKa = 10.21IGDD40 pKa = 5.4DD41 pKa = 3.19ILVATIIEE49 pKa = 4.34EE50 pKa = 4.3DD51 pKa = 3.68EE52 pKa = 4.42SIQIIGLLSEE62 pKa = 3.85YY63 pKa = 10.22TYY65 pKa = 11.58YY66 pKa = 11.42LDD68 pKa = 6.17LFMQAMTEE76 pKa = 3.97DD77 pKa = 3.77SEE79 pKa = 4.96VRR81 pKa = 11.84DD82 pKa = 3.55LVFGLFNYY90 pKa = 9.34YY91 pKa = 10.0EE92 pKa = 4.28KK93 pKa = 10.55EE94 pKa = 3.83FPYY97 pKa = 10.0MKK99 pKa = 10.1EE100 pKa = 3.59YY101 pKa = 10.69EE102 pKa = 4.21LRR104 pKa = 11.84LKK106 pKa = 10.7SGNNN110 pKa = 3.27

Molecular weight: 12.98 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4R6ICV0|A0A4R6ICV0_9MOLU Uncharacterized protein OS=Mycoplasma testudineum OX=244584 GN=EI74_0617 PE=4 SV=1
MM1 pKa = 7.37KK2 pKa = 10.25RR3 pKa = 11.84RR4 pKa = 11.84SKK6 pKa = 10.62RR7 pKa = 11.84MKK9 pKa = 9.14VRR11 pKa = 11.84KK12 pKa = 8.92SRR14 pKa = 11.84KK15 pKa = 9.15RR16 pKa = 11.84NWLLRR21 pKa = 11.84QSILFVKK28 pKa = 9.3EE29 pKa = 3.97LKK31 pKa = 9.79RR32 pKa = 11.84VRR34 pKa = 11.84WPTLRR39 pKa = 11.84VSWVAFGKK47 pKa = 10.45IIIFTIIFSLFVFALATGATAALNAIGVGVSNGG80 pKa = 3.18

Molecular weight: 9.21 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

783

0

783

285863

37

4508

365.1

41.52

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.801 ± 0.096

0.235 ± 0.013

5.265 ± 0.071

6.28 ± 0.098

5.536 ± 0.083

4.793 ± 0.084

1.378 ± 0.04

9.623 ± 0.096

9.204 ± 0.111

9.318 ± 0.081

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.834 ± 0.041

7.737 ± 0.117

2.694 ± 0.052

3.268 ± 0.059

3.02 ± 0.061

7.549 ± 0.091

5.615 ± 0.13

5.896 ± 0.076

0.961 ± 0.028

3.992 ± 0.058
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski