Pea enation mosaic virus-1 (strain WSG) (PEMV-1)

Taxonomy: Viruses; Riboviria; Orthornavirae; Pisuviricota; Pisoniviricetes; Sobelivirales; Solemoviridae; Enamovirus; Pea enation mosaic virus 1

Average proteome isoelectric point is 7.64

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|Q84710|P1_PEMVW Protein P1 OS=Pea enation mosaic virus-1 (strain WSG) OX=693989 GN=ORF1 PE=1 SV=1
MM1 pKa = 7.47HH2 pKa = 7.61GIEE5 pKa = 4.54QPQLPLDD12 pKa = 3.88YY13 pKa = 10.96VHH15 pKa = 7.27RR16 pKa = 11.84CASTSFLLASLDD28 pKa = 3.7GLLSEE33 pKa = 4.94ARR35 pKa = 11.84EE36 pKa = 4.07LSGPLALITSSYY48 pKa = 9.67YY49 pKa = 11.01LLVSIALCWAIPGSFWYY66 pKa = 10.31RR67 pKa = 11.84PGCWLQPVSGRR78 pKa = 11.84NLIFCGPTEE87 pKa = 3.89ALQRR91 pKa = 11.84FRR93 pKa = 11.84LYY95 pKa = 10.64AARR98 pKa = 11.84LGLVLSEE105 pKa = 3.75NCPRR109 pKa = 11.84HH110 pKa = 5.4GQSAAITLQSYY121 pKa = 7.64WALPNNIWMDD131 pKa = 3.77MAQLDD136 pKa = 4.73LLTFSMPIANTFAYY150 pKa = 10.45LADD153 pKa = 4.07CEE155 pKa = 4.92ARR157 pKa = 11.84FPPIVEE163 pKa = 4.53GVGSAYY169 pKa = 10.26YY170 pKa = 10.78VPTLLGLTHH179 pKa = 6.64QDD181 pKa = 2.8PRR183 pKa = 11.84LYY185 pKa = 10.65LALRR189 pKa = 11.84RR190 pKa = 11.84RR191 pKa = 11.84NLDD194 pKa = 3.24LSGEE198 pKa = 4.13PHH200 pKa = 6.56RR201 pKa = 11.84VRR203 pKa = 11.84PGVLEE208 pKa = 4.57SMALLCSSVRR218 pKa = 11.84STSRR222 pKa = 11.84SRR224 pKa = 11.84QIPPLYY230 pKa = 10.31GSVLHH235 pKa = 6.58HH236 pKa = 6.11VLGLAEE242 pKa = 4.39RR243 pKa = 11.84DD244 pKa = 3.94CILFDD249 pKa = 3.51TDD251 pKa = 4.27SNYY254 pKa = 10.45SSYY257 pKa = 7.4THH259 pKa = 7.05RR260 pKa = 11.84VLEE263 pKa = 3.99QDD265 pKa = 3.89RR266 pKa = 11.84NRR268 pKa = 11.84ADD270 pKa = 3.13QSLFSIDD277 pKa = 3.72LEE279 pKa = 4.42YY280 pKa = 11.55VHH282 pKa = 7.37DD283 pKa = 5.02LEE285 pKa = 6.37LIALGYY291 pKa = 10.13SDD293 pKa = 6.09EE294 pKa = 5.43DD295 pKa = 6.14DD296 pKa = 4.02EE297 pKa = 6.55DD298 pKa = 5.02LDD300 pKa = 4.49NFFF303 pKa = 5.65

Molecular weight: 34.12 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|P29154|RDRP_PEMVW Protein P1-P2 OS=Pea enation mosaic virus-1 (strain WSG) OX=693989 GN=ORF1/ORF2 PE=3 SV=2
MM1 pKa = 7.25PTRR4 pKa = 11.84SRR6 pKa = 11.84SKK8 pKa = 9.39ANQRR12 pKa = 11.84RR13 pKa = 11.84RR14 pKa = 11.84RR15 pKa = 11.84PRR17 pKa = 11.84RR18 pKa = 11.84VVVVAPSMAQPRR30 pKa = 11.84TQSRR34 pKa = 11.84RR35 pKa = 11.84PRR37 pKa = 11.84RR38 pKa = 11.84RR39 pKa = 11.84NKK41 pKa = 10.39RR42 pKa = 11.84GGGLNGSHH50 pKa = 5.93TVDD53 pKa = 4.09FSMVHH58 pKa = 6.25GPFNGNATGTVKK70 pKa = 10.84FGPSSDD76 pKa = 3.84CQCIKK81 pKa = 11.1GNLAAYY87 pKa = 8.97QKK89 pKa = 11.0YY90 pKa = 9.83RR91 pKa = 11.84IVWLKK96 pKa = 10.59VVYY99 pKa = 10.23QSEE102 pKa = 4.02AAATDD107 pKa = 3.93RR108 pKa = 11.84GCIAYY113 pKa = 9.48HH114 pKa = 6.44VDD116 pKa = 2.87TSTTKK121 pKa = 10.26KK122 pKa = 10.46AADD125 pKa = 3.62VVLLDD130 pKa = 3.38TWNIRR135 pKa = 11.84SNGSATFGRR144 pKa = 11.84EE145 pKa = 3.72ILGDD149 pKa = 3.82QPWYY153 pKa = 10.36EE154 pKa = 4.24SNKK157 pKa = 10.14DD158 pKa = 3.31QFFFLYY164 pKa = 9.98RR165 pKa = 11.84GTGGTDD171 pKa = 2.94VAGHH175 pKa = 5.26YY176 pKa = 9.54RR177 pKa = 11.84ISGRR181 pKa = 11.84IQLMNASLL189 pKa = 3.54

Molecular weight: 21.13 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5

0

5

2926

189

1181

585.2

65.21

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.972 ± 0.232

2.016 ± 0.143

4.99 ± 0.359

4.887 ± 0.561

3.691 ± 0.246

7.04 ± 0.589

1.777 ± 0.196

4.375 ± 0.132

5.195 ± 0.745

9.877 ± 1.365

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.085 ± 0.174

3.008 ± 0.368

6.528 ± 0.547

4.17 ± 0.114

6.699 ± 0.735

9.364 ± 0.193

6.425 ± 0.42

5.742 ± 0.277

2.495 ± 0.189

2.666 ± 0.538
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski