Mycobacterium phage Charlie

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Nclasvirinae; Charlievirus; Mycobacterium virus Charlie

Average proteome isoelectric point is 6.17

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 68 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|G1FTW0|G1FTW0_9CAUD Uncharacterized protein OS=Mycobacterium phage Charlie OX=1056830 GN=22 PE=4 SV=1
MM1 pKa = 7.73AKK3 pKa = 10.28LDD5 pKa = 3.88TLTDD9 pKa = 3.76DD10 pKa = 3.74FTVYY14 pKa = 10.9DD15 pKa = 4.1PGKK18 pKa = 7.82WTIDD22 pKa = 3.45DD23 pKa = 4.19SVGGDD28 pKa = 3.05IDD30 pKa = 4.0IGDD33 pKa = 3.53GRR35 pKa = 11.84AAINTNAGIEE45 pKa = 4.51GVFSVGAYY53 pKa = 10.22DD54 pKa = 4.68LLEE57 pKa = 4.63SYY59 pKa = 11.22AFAQFISFGFLYY71 pKa = 9.7PGKK74 pKa = 10.79GFGFSLNINSGGPNEE89 pKa = 4.37DD90 pKa = 2.86SAMFLCTEE98 pKa = 4.28SEE100 pKa = 4.55TEE102 pKa = 3.85GEE104 pKa = 4.27YY105 pKa = 11.3NLICLDD111 pKa = 3.54GAGFGAEE118 pKa = 4.19GTPIEE123 pKa = 4.4PVATGVTYY131 pKa = 10.74DD132 pKa = 3.82PAIHH136 pKa = 5.58SWLRR140 pKa = 11.84IRR142 pKa = 11.84EE143 pKa = 4.28SGGTIYY149 pKa = 10.47WEE151 pKa = 4.34TSPDD155 pKa = 3.6TAPLEE160 pKa = 4.22WSVMASGAHH169 pKa = 5.76TFTSAQATVQLGGVFAPAAFTVSSFNIAPTPPIPANAGNFFAFFF213 pKa = 4.14

Molecular weight: 22.45 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|G1FU02|G1FU02_9CAUD Uncharacterized protein OS=Mycobacterium phage Charlie OX=1056830 GN=64 PE=4 SV=1
MM1 pKa = 8.2DD2 pKa = 4.27EE3 pKa = 4.18PRR5 pKa = 11.84PLRR8 pKa = 11.84VHH10 pKa = 5.32RR11 pKa = 11.84TARR14 pKa = 11.84RR15 pKa = 11.84RR16 pKa = 11.84LEE18 pKa = 3.89RR19 pKa = 11.84RR20 pKa = 11.84NGATIPQRR28 pKa = 11.84QPHH31 pKa = 5.43QMPPRR36 pKa = 11.84DD37 pKa = 3.39APRR40 pKa = 11.84RR41 pKa = 11.84HH42 pKa = 5.82QLDD45 pKa = 3.66TARR48 pKa = 11.84PAPMPRR54 pKa = 11.84LALQPLRR61 pKa = 11.84RR62 pKa = 11.84RR63 pKa = 11.84DD64 pKa = 3.41KK65 pKa = 10.7QLRR68 pKa = 11.84PPQLPRR74 pKa = 11.84PTPATGEE81 pKa = 4.15PPVTRR86 pKa = 11.84DD87 pKa = 2.92KK88 pKa = 11.34SSRR91 pKa = 11.84TLTAGQLIAQLLKK104 pKa = 10.88VPADD108 pKa = 3.81TPVVMSQEE116 pKa = 4.28DD117 pKa = 4.52EE118 pKa = 4.17PVGCYY123 pKa = 8.77GVRR126 pKa = 11.84SVEE129 pKa = 3.99LEE131 pKa = 3.73EE132 pKa = 4.06MRR134 pKa = 11.84RR135 pKa = 11.84DD136 pKa = 3.47PTYY139 pKa = 11.28ADD141 pKa = 3.67GPFGRR146 pKa = 11.84DD147 pKa = 2.83SWHH150 pKa = 5.66STLYY154 pKa = 10.63SCAGYY159 pKa = 10.31DD160 pKa = 3.46PPQQVVFLGPEE171 pKa = 4.22RR172 pKa = 11.84PWQPTIDD179 pKa = 4.13GEE181 pKa = 4.36PEE183 pKa = 3.64RR184 pKa = 11.84PAIEE188 pKa = 4.39SGDD191 pKa = 3.57RR192 pKa = 11.84RR193 pKa = 3.77

Molecular weight: 22.0 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

68

0

68

14021

38

1047

206.2

22.37

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.303 ± 0.816

1.255 ± 0.163

6.533 ± 0.214

5.563 ± 0.411

3.06 ± 0.196

8.666 ± 0.518

2.083 ± 0.19

4.557 ± 0.214

3.017 ± 0.229

7.931 ± 0.366

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.033 ± 0.135

3.095 ± 0.227

6.098 ± 0.295

4.015 ± 0.228

6.911 ± 0.463

5.178 ± 0.219

6.768 ± 0.336

6.747 ± 0.259

1.869 ± 0.154

2.318 ± 0.192
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski