Shayang Fly Virus 3

Taxonomy: Viruses; Riboviria; Orthornavirae; Negarnaviricota; Haploviricotina; Monjiviricetes; Mononegavirales; Rhabdoviridae; Alphadrosrhavirus; Shayang alphadrosrhavirus

Average proteome isoelectric point is 6.86

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0B5KXF0|A0A0B5KXF0_9RHAB ORF2 OS=Shayang Fly Virus 3 OX=1608067 GN=ORF2 PE=4 SV=1
MM1 pKa = 7.36SFSISRR7 pKa = 11.84QICQLEE13 pKa = 4.23NCIHH17 pKa = 6.44GPEE20 pKa = 4.01EE21 pKa = 4.05HH22 pKa = 7.28PISVDD27 pKa = 3.28CMEE30 pKa = 4.98DD31 pKa = 3.42QNEE34 pKa = 4.13EE35 pKa = 4.0EE36 pKa = 5.22LLNLDD41 pKa = 4.86PDD43 pKa = 4.02DD44 pKa = 5.31QAILDD49 pKa = 3.6NAQFLKK55 pKa = 10.76ASSHH59 pKa = 7.06FYY61 pKa = 9.96GQEE64 pKa = 3.73DD65 pKa = 3.77ATGKK69 pKa = 9.98IEE71 pKa = 3.95TLLRR75 pKa = 11.84SDD77 pKa = 4.54SLDD80 pKa = 3.77DD81 pKa = 3.88NFAAYY86 pKa = 10.41VNSSNEE92 pKa = 4.13DD93 pKa = 3.32KK94 pKa = 11.17DD95 pKa = 3.92KK96 pKa = 11.55EE97 pKa = 4.41NYY99 pKa = 9.82NPDD102 pKa = 3.21VNQNDD107 pKa = 3.57SLLRR111 pKa = 11.84KK112 pKa = 9.78SEE114 pKa = 3.76GGEE117 pKa = 5.1DD118 pKa = 2.99INQAINNFYY127 pKa = 10.61QYY129 pKa = 9.58WSKK132 pKa = 11.26PEE134 pKa = 3.92NQEE137 pKa = 3.67ILYY140 pKa = 7.15KK141 pKa = 10.44TPLIVFDD148 pKa = 5.56SIIKK152 pKa = 10.21DD153 pKa = 3.38PRR155 pKa = 11.84FEE157 pKa = 4.56NYY159 pKa = 10.31CNMFSSCRR167 pKa = 11.84LKK169 pKa = 10.13FHH171 pKa = 6.05QLGVEE176 pKa = 4.39RR177 pKa = 11.84GRR179 pKa = 11.84DD180 pKa = 3.59LMIHH184 pKa = 6.85FISEE188 pKa = 3.69RR189 pKa = 11.84NIRR192 pKa = 11.84YY193 pKa = 7.62FQNFDD198 pKa = 2.83IMLFGANTVSQTANQKK214 pKa = 10.35DD215 pKa = 3.92LEE217 pKa = 4.21LLKK220 pKa = 10.98VEE222 pKa = 4.85FEE224 pKa = 4.68SKK226 pKa = 10.7VSFLVDD232 pKa = 3.29SVKK235 pKa = 10.05TVEE238 pKa = 4.08EE239 pKa = 4.44HH240 pKa = 6.6IKK242 pKa = 10.12TIDD245 pKa = 3.48SSHH248 pKa = 6.11EE249 pKa = 3.85ALRR252 pKa = 11.84DD253 pKa = 3.65TINCVSEE260 pKa = 4.11AAHH263 pKa = 6.15GLSAAKK269 pKa = 10.13RR270 pKa = 11.84SFEE273 pKa = 4.06LLQEE277 pKa = 4.42RR278 pKa = 11.84DD279 pKa = 3.36PSYY282 pKa = 11.66SPVISNTARR291 pKa = 11.84DD292 pKa = 3.94MPKK295 pKa = 10.28KK296 pKa = 10.28SVSIPKK302 pKa = 10.33LKK304 pKa = 9.4TVYY307 pKa = 10.21KK308 pKa = 10.04KK309 pKa = 10.11EE310 pKa = 4.04HH311 pKa = 6.0LSIPIGMNDD320 pKa = 3.93EE321 pKa = 4.58LVISQISVYY330 pKa = 10.99NKK332 pKa = 10.34GLTLSDD338 pKa = 4.0FKK340 pKa = 11.37LFEE343 pKa = 4.72GLSKK347 pKa = 10.44MKK349 pKa = 10.94AEE351 pKa = 4.19VFLRR355 pKa = 11.84ALNLPWALLTKK366 pKa = 9.2WANSSPDD373 pKa = 3.23NSSIGIARR381 pKa = 11.84TLIPMIMNLVPLPAKK396 pKa = 10.83VEE398 pKa = 4.27FF399 pKa = 4.45

Molecular weight: 45.47 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0B5KK44|A0A0B5KK44_9RHAB Glycoprotein OS=Shayang Fly Virus 3 OX=1608067 GN=G PE=4 SV=1
MM1 pKa = 7.62LGLFSTSSRR10 pKa = 11.84PISPISFCNLNSRR23 pKa = 11.84SLSSIKK29 pKa = 9.75TIYY32 pKa = 7.5FTPSSSSFSTMTEE45 pKa = 3.75PSTSKK50 pKa = 10.95SLVTSIPHH58 pKa = 5.49YY59 pKa = 10.99SPLQTEE65 pKa = 4.45QISLVIKK72 pKa = 10.21GAIDD76 pKa = 3.47VSGPKK81 pKa = 9.94LDD83 pKa = 3.66FVEE86 pKa = 4.81FLWRR90 pKa = 11.84VCCVLAYY97 pKa = 8.81EE98 pKa = 4.27ATRR101 pKa = 11.84LKK103 pKa = 10.69SVSRR107 pKa = 11.84NTQYY111 pKa = 11.41LVASILYY118 pKa = 9.27EE119 pKa = 3.97EE120 pKa = 5.08LRR122 pKa = 11.84SSSYY126 pKa = 11.11LGTFSSPDD134 pKa = 3.11IGPTCEE140 pKa = 4.2LLCPIEE146 pKa = 4.28VKK148 pKa = 10.78GFTEE152 pKa = 5.04FPIEE156 pKa = 4.22SSSFPQIRR164 pKa = 11.84KK165 pKa = 8.67SLNRR169 pKa = 11.84VFRR172 pKa = 11.84YY173 pKa = 8.26QVKK176 pKa = 9.41PGSILRR182 pKa = 11.84IRR184 pKa = 11.84AGLTIQACKK193 pKa = 10.53FNDD196 pKa = 3.63KK197 pKa = 9.7YY198 pKa = 11.41RR199 pKa = 11.84EE200 pKa = 3.67MARR203 pKa = 11.84IKK205 pKa = 10.6GYY207 pKa = 10.94VPLLPPSNEE216 pKa = 3.59HH217 pKa = 6.45LISYY221 pKa = 9.35FSLLEE226 pKa = 3.91NSIEE230 pKa = 4.09TAGAYY235 pKa = 9.1NEE237 pKa = 4.35KK238 pKa = 10.51DD239 pKa = 2.82ISSFIASGIDD249 pKa = 3.15ALAEE253 pKa = 4.1KK254 pKa = 10.16EE255 pKa = 4.04ADD257 pKa = 3.7PPLSFIKK264 pKa = 10.75LIRR267 pKa = 11.84DD268 pKa = 3.66KK269 pKa = 10.81IFKK272 pKa = 9.96QNPPASIGPGKK283 pKa = 10.29ASSGTKK289 pKa = 10.08PPDD292 pKa = 3.28GLRR295 pKa = 11.84ILTQSKK301 pKa = 10.72LKK303 pKa = 10.32GSKK306 pKa = 10.11YY307 pKa = 10.75DD308 pKa = 3.66LALCEE313 pKa = 4.33STSSIKK319 pKa = 10.41KK320 pKa = 10.0SEE322 pKa = 4.39SINN325 pKa = 3.46

Molecular weight: 35.81 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5

0

5

4082

325

2231

816.4

92.92

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.532 ± 0.733

1.788 ± 0.052

4.998 ± 0.335

5.194 ± 0.458

5.781 ± 0.42

4.483 ± 0.308

2.327 ± 0.305

8.305 ± 0.483

6.835 ± 0.261

10.828 ± 0.502

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.597 ± 0.301

5.512 ± 0.329

4.483 ± 0.321

3.503 ± 0.276

3.944 ± 0.246

10.289 ± 0.764

5.267 ± 0.395

4.532 ± 0.349

1.249 ± 0.206

3.552 ± 0.206
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski