Halomonas sp. ND22Bw

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Oceanospirillales; Halomonadaceae; Halomonas; unclassified Halomonas

Average proteome isoelectric point is 6.12

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4859 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2P7P843|A0A2P7P843_9GAMM FUSC family protein OS=Halomonas sp. ND22Bw OX=2054178 GN=CVH10_09575 PE=4 SV=1
MM1 pKa = 7.71KK2 pKa = 9.06KK3 pKa = 8.27TLLATAIAGAMAASGAQAATVYY25 pKa = 10.61NQDD28 pKa = 3.01GTKK31 pKa = 10.46LDD33 pKa = 3.42IYY35 pKa = 11.34GNLQIAYY42 pKa = 9.67RR43 pKa = 11.84SIEE46 pKa = 4.09TADD49 pKa = 3.56EE50 pKa = 4.21STGDD54 pKa = 3.39VEE56 pKa = 5.07SRR58 pKa = 11.84DD59 pKa = 4.28DD60 pKa = 3.61IFDD63 pKa = 3.86NGSTLGVAGEE73 pKa = 3.99HH74 pKa = 6.35VIYY77 pKa = 10.78DD78 pKa = 3.7GLTGYY83 pKa = 10.22FKK85 pKa = 11.68YY86 pKa = 10.38EE87 pKa = 3.83FEE89 pKa = 4.57GNADD93 pKa = 3.61EE94 pKa = 6.21AKK96 pKa = 10.79DD97 pKa = 3.61QGGLNSGDD105 pKa = 3.2QAYY108 pKa = 10.31FGLKK112 pKa = 10.51GNFGDD117 pKa = 4.77ARR119 pKa = 11.84LGSWDD124 pKa = 4.23PLIDD128 pKa = 5.15DD129 pKa = 5.77WIQDD133 pKa = 3.91PITNNEE139 pKa = 3.92YY140 pKa = 10.91FDD142 pKa = 4.42VSDD145 pKa = 3.45SSSRR149 pKa = 11.84IVGVEE154 pKa = 3.93DD155 pKa = 3.26SRR157 pKa = 11.84EE158 pKa = 4.18GNKK161 pKa = 9.39LQYY164 pKa = 9.29MSPSFSGFQFAVGTQYY180 pKa = 11.15EE181 pKa = 4.3GDD183 pKa = 3.77AEE185 pKa = 4.14YY186 pKa = 10.77DD187 pKa = 3.38ASNTASISGTNGANLTVSEE206 pKa = 4.76KK207 pKa = 10.25RR208 pKa = 11.84DD209 pKa = 3.53SNASFFGGVKK219 pKa = 8.36YY220 pKa = 8.62TAGAFSIAAVYY231 pKa = 10.89DD232 pKa = 3.62NLDD235 pKa = 4.02NNDD238 pKa = 2.77GRR240 pKa = 11.84YY241 pKa = 10.49DD242 pKa = 3.76GVDD245 pKa = 2.93GDD247 pKa = 4.77GNVISGDD254 pKa = 3.38FDD256 pKa = 4.35AGEE259 pKa = 4.18QYY261 pKa = 11.05GITAQYY267 pKa = 8.75TWDD270 pKa = 3.81TLRR273 pKa = 11.84VAAKK277 pKa = 9.71VEE279 pKa = 4.2RR280 pKa = 11.84FKK282 pKa = 11.48SDD284 pKa = 2.86NDD286 pKa = 3.48FVADD290 pKa = 3.72TNFYY294 pKa = 11.11GLGARR299 pKa = 11.84YY300 pKa = 9.66GYY302 pKa = 11.31GNGDD306 pKa = 2.74IYY308 pKa = 11.26GAYY311 pKa = 9.92QYY313 pKa = 11.46VDD315 pKa = 3.28VGGGDD320 pKa = 4.89LIDD323 pKa = 4.14TADD326 pKa = 4.02DD327 pKa = 4.17ALTSGDD333 pKa = 3.48WPNEE337 pKa = 4.05RR338 pKa = 11.84EE339 pKa = 4.02DD340 pKa = 3.58EE341 pKa = 4.41SYY343 pKa = 11.62NEE345 pKa = 3.86IMLGATYY352 pKa = 10.55NVSDD356 pKa = 3.38AMYY359 pKa = 9.4TFVEE363 pKa = 4.31AAWYY367 pKa = 9.89DD368 pKa = 3.55RR369 pKa = 11.84DD370 pKa = 3.69EE371 pKa = 4.55DD372 pKa = 3.87VGDD375 pKa = 4.18GVAVGAVYY383 pKa = 10.44LFF385 pKa = 4.21

Molecular weight: 41.49 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2P7PAJ7|A0A2P7PAJ7_9GAMM ATP-dependent DNA helicase RecG OS=Halomonas sp. ND22Bw OX=2054178 GN=recG PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.51RR3 pKa = 11.84TFQPSVLKK11 pKa = 10.42RR12 pKa = 11.84KK13 pKa = 9.04RR14 pKa = 11.84AHH16 pKa = 6.12GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.3NGRR28 pKa = 11.84AVLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.93GRR39 pKa = 11.84KK40 pKa = 8.85RR41 pKa = 11.84LSAA44 pKa = 3.96

Molecular weight: 5.12 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4859

0

4859

1204888

20

1644

248.0

27.08

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.132 ± 0.052

0.877 ± 0.012

6.1 ± 0.038

6.671 ± 0.046

3.337 ± 0.026

8.645 ± 0.037

2.398 ± 0.019

4.231 ± 0.032

2.342 ± 0.034

11.457 ± 0.054

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.42 ± 0.019

2.226 ± 0.02

5.09 ± 0.027

3.465 ± 0.024

7.797 ± 0.051

5.022 ± 0.025

4.883 ± 0.025

7.219 ± 0.035

1.458 ± 0.019

2.229 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski