Porcine polyomavirus

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cossaviricota; Papovaviricetes; Sepolyvirales; Polyomaviridae; unclassified Polyomaviridae

Average proteome isoelectric point is 6.24

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2P1K4R5|A0A2P1K4R5_9POLY VP2 OS=Porcine polyomavirus OX=2126099 PE=4 SV=1
MM1 pKa = 7.29GAILAIPEE9 pKa = 3.83ILAAAVEE16 pKa = 4.65GAAEE20 pKa = 4.68AIEE23 pKa = 4.13IAGSFGAVASGEE35 pKa = 3.94GLAAVEE41 pKa = 4.4TLVQSATLGGEE52 pKa = 3.99YY53 pKa = 10.24LGASLAEE60 pKa = 3.83QAAALGIPDD69 pKa = 3.84SAFTLLSQTPEE80 pKa = 3.76LANTFTAISGLVNAGAGLAGGVATYY105 pKa = 10.44LGNSGGLPGQEE116 pKa = 3.91AGGGGSGLYY125 pKa = 9.61PYY127 pKa = 10.81YY128 pKa = 9.47PGHH131 pKa = 6.83GGHH134 pKa = 6.48FHH136 pKa = 6.63PTHH139 pKa = 6.13HH140 pKa = 6.1TMEE143 pKa = 4.72LAVIPPYY150 pKa = 10.23QLEE153 pKa = 4.18SGIPGIPDD161 pKa = 3.0WVLNLLPEE169 pKa = 4.63LPSLSDD175 pKa = 3.19IVSGIANGLWTSYY188 pKa = 10.65YY189 pKa = 9.26RR190 pKa = 11.84TGTEE194 pKa = 4.47LIRR197 pKa = 11.84RR198 pKa = 11.84TASDD202 pKa = 3.32EE203 pKa = 4.03LQRR206 pKa = 11.84LLGDD210 pKa = 4.07LSTGLRR216 pKa = 11.84QASEE220 pKa = 3.93AAQQYY225 pKa = 9.51IQDD228 pKa = 4.3ADD230 pKa = 3.73PVNALVRR237 pKa = 11.84QIEE240 pKa = 4.2RR241 pKa = 11.84VQVQRR246 pKa = 11.84RR247 pKa = 11.84VDD249 pKa = 3.54SARR252 pKa = 11.84LIQEE256 pKa = 4.17AEE258 pKa = 4.09TGVLNLGEE266 pKa = 4.07IVQRR270 pKa = 11.84VADD273 pKa = 4.21TTRR276 pKa = 11.84GAAGSVGQIATDD288 pKa = 4.43LAQLPVDD295 pKa = 4.96GYY297 pKa = 10.1NALSGGVQRR306 pKa = 11.84LGQWISMSGPTGGTLHH322 pKa = 6.68YY323 pKa = 10.54SFPHH327 pKa = 4.91WVLYY331 pKa = 10.64VLDD334 pKa = 4.38EE335 pKa = 4.5LQLDD339 pKa = 4.45FPPKK343 pKa = 9.87QGIKK347 pKa = 10.1RR348 pKa = 11.84GLPEE352 pKa = 5.31DD353 pKa = 4.01PQPPEE358 pKa = 3.9GDD360 pKa = 3.3VYY362 pKa = 10.24QEE364 pKa = 3.54RR365 pKa = 11.84KK366 pKa = 7.53TRR368 pKa = 11.84LRR370 pKa = 11.84TYY372 pKa = 10.89KK373 pKa = 10.47SGPKK377 pKa = 8.29TANPNKK383 pKa = 9.64KK384 pKa = 9.53RR385 pKa = 11.84RR386 pKa = 11.84RR387 pKa = 3.69

Molecular weight: 40.68 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2P1K4Q5|A0A2P1K4Q5_9POLY Large T antigen OS=Porcine polyomavirus OX=2126099 PE=4 SV=1
MM1 pKa = 7.47SWMNFSLISPQNRR14 pKa = 11.84ASRR17 pKa = 11.84EE18 pKa = 3.76ACLRR22 pKa = 11.84IHH24 pKa = 6.07SHH26 pKa = 6.47LKK28 pKa = 10.49GMSTKK33 pKa = 10.1KK34 pKa = 10.26GKK36 pKa = 9.97RR37 pKa = 11.84VSAPTRR43 pKa = 11.84VVQRR47 pKa = 11.84LPTQIRR53 pKa = 11.84KK54 pKa = 10.16GGVDD58 pKa = 3.39VLATVPLDD66 pKa = 3.04QHH68 pKa = 5.56TEE70 pKa = 4.05YY71 pKa = 10.93KK72 pKa = 10.51VEE74 pKa = 4.42LFLKK78 pKa = 8.97PTFATTDD85 pKa = 3.39GNGVFQSIGGPLTNGQEE102 pKa = 4.24PNVGEE107 pKa = 4.14LLLYY111 pKa = 10.22SLGMVQPPEE120 pKa = 3.94IPNQVDD126 pKa = 3.87EE127 pKa = 4.62NSMIVWEE134 pKa = 4.62LYY136 pKa = 9.8RR137 pKa = 11.84VEE139 pKa = 4.34TEE141 pKa = 4.02VLFAPKK147 pKa = 9.58TFSTGYY153 pKa = 9.45TSAQKK158 pKa = 10.85NIGGVEE164 pKa = 4.23GPQFYY169 pKa = 9.69FWAVGGEE176 pKa = 4.0PLDD179 pKa = 3.56VLGIKK184 pKa = 9.15SRR186 pKa = 11.84KK187 pKa = 8.69SVTYY191 pKa = 7.44PTGVQAPPNGEE202 pKa = 4.1DD203 pKa = 3.5LVTEE207 pKa = 3.86NTVRR211 pKa = 11.84RR212 pKa = 11.84KK213 pKa = 9.39VDD215 pKa = 3.38KK216 pKa = 10.46PQFPCDD222 pKa = 3.3CWLPDD227 pKa = 4.06PSRR230 pKa = 11.84NDD232 pKa = 2.88NTRR235 pKa = 11.84YY236 pKa = 8.56FGRR239 pKa = 11.84VIGGSQTPPVLTIGNSSTVPVVDD262 pKa = 4.22EE263 pKa = 4.46YY264 pKa = 11.84GVGVLLLQGRR274 pKa = 11.84LYY276 pKa = 9.84VTSCDD281 pKa = 3.53MLGTVAEE288 pKa = 4.42VNEE291 pKa = 4.13ATLAPEE297 pKa = 4.2SNSRR301 pKa = 11.84RR302 pKa = 11.84IHH304 pKa = 5.9AGSGRR309 pKa = 11.84FFRR312 pKa = 11.84LHH314 pKa = 4.93FRR316 pKa = 11.84QRR318 pKa = 11.84KK319 pKa = 7.63VKK321 pKa = 10.47NPYY324 pKa = 9.06TINLLYY330 pKa = 10.54KK331 pKa = 10.35QVFNKK336 pKa = 10.42PNDD339 pKa = 3.71DD340 pKa = 3.71FVGQTGVTEE349 pKa = 3.94VTMTQEE355 pKa = 4.24TQPLPTILEE364 pKa = 4.48GTVGTQGNLVTEE376 pKa = 4.46ALRR379 pKa = 11.84RR380 pKa = 11.84GTAVLQTTTPLVNTQSLL397 pKa = 3.7

Molecular weight: 43.71 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4

0

4

1634

195

655

408.5

46.03

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.263 ± 1.62

2.142 ± 0.989

5.141 ± 0.805

6.548 ± 0.396

3.55 ± 0.743

8.078 ± 1.673

2.02 ± 0.314

4.162 ± 0.307

5.447 ± 1.291

10.71 ± 0.562

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.326 ± 0.563

4.651 ± 0.657

5.202 ± 0.939

4.835 ± 0.51

5.263 ± 0.278

5.447 ± 0.332

6.426 ± 1.132

6.977 ± 1.064

1.836 ± 0.67

3.978 ± 0.548
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski