Vibrio phage pVp-1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Demerecviridae; Ermolyevavirinae; Vipunavirus; Vibrio virus pVp1

Average proteome isoelectric point is 6.37

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 157 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|H6WXM2|H6WXM2_9CAUD Uncharacterized protein OS=Vibrio phage pVp-1 OX=1150989 GN=pVp-1_0131 PE=4 SV=1
MM1 pKa = 7.81AFNCNEE7 pKa = 4.12TPTLLSLDD15 pKa = 3.79SIVRR19 pKa = 11.84YY20 pKa = 9.94VKK22 pKa = 10.01EE23 pKa = 3.94EE24 pKa = 3.67RR25 pKa = 11.84SSFVEE30 pKa = 4.5DD31 pKa = 3.81PEE33 pKa = 4.36STIWYY38 pKa = 9.03IDD40 pKa = 3.47ADD42 pKa = 3.85NEE44 pKa = 4.26LAVMVHH50 pKa = 6.7PAFCMMFYY58 pKa = 10.52NSDD61 pKa = 3.22MGIEE65 pKa = 4.42GMCDD69 pKa = 3.6LDD71 pKa = 4.76CVTDD75 pKa = 4.39LSAMLDD81 pKa = 3.49NYY83 pKa = 11.36GLDD86 pKa = 3.44TSSMVWRR93 pKa = 11.84EE94 pKa = 3.62IGIDD98 pKa = 3.26QIDD101 pKa = 3.62YY102 pKa = 11.08HH103 pKa = 6.43FVNN106 pKa = 4.49

Molecular weight: 12.16 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|H6WXF4|H6WXF4_9CAUD Uncharacterized protein OS=Vibrio phage pVp-1 OX=1150989 GN=pVp-1_0063 PE=4 SV=1
MM1 pKa = 6.91WHH3 pKa = 5.77RR4 pKa = 11.84HH5 pKa = 4.16KK6 pKa = 11.18LRR8 pKa = 11.84VNWHH12 pKa = 6.34RR13 pKa = 11.84RR14 pKa = 11.84LKK16 pKa = 10.95DD17 pKa = 3.24DD18 pKa = 6.37DD19 pKa = 3.64IAIEE23 pKa = 3.99NGFTNRR29 pKa = 11.84LFRR32 pKa = 11.84QTDD35 pKa = 3.28VYY37 pKa = 10.69MYY39 pKa = 10.81DD40 pKa = 3.97LSWWDD45 pKa = 3.51YY46 pKa = 10.8PSRR49 pKa = 11.84TVQRR53 pKa = 11.84SWKK56 pKa = 9.17SQSKK60 pKa = 9.83KK61 pKa = 8.87RR62 pKa = 11.84KK63 pKa = 6.25QWEE66 pKa = 3.91RR67 pKa = 3.3

Molecular weight: 8.67 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

157

0

157

31641

40

1028

201.5

22.75

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.61 ± 0.255

1.204 ± 0.088

6.384 ± 0.164

7.038 ± 0.216

4.011 ± 0.127

6.397 ± 0.204

1.893 ± 0.118

6.242 ± 0.147

7.61 ± 0.191

8.394 ± 0.238

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.69 ± 0.111

4.959 ± 0.139

3.467 ± 0.133

3.783 ± 0.146

4.504 ± 0.162

5.787 ± 0.183

6.172 ± 0.215

6.476 ± 0.174

1.422 ± 0.081

3.957 ± 0.15
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski