Lactococcus garvieae (strain Lg2) (Enterococcus seriolicida)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Lactobacillales; Streptococcaceae; Lactococcus; Lactococcus garvieae

Average proteome isoelectric point is 6.39

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1938 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|F9VEW7|F9VEW7_LACGL Uncharacterized protein OS=Lactococcus garvieae (strain Lg2) OX=420890 GN=LCGL_1408 PE=4 SV=1
MM1 pKa = 7.28EE2 pKa = 4.54TPKK5 pKa = 10.31IYY7 pKa = 10.14IVNLSSYY14 pKa = 11.34NNGNTRR20 pKa = 11.84GKK22 pKa = 9.46WYY24 pKa = 9.24EE25 pKa = 3.98LPVDD29 pKa = 3.61MGRR32 pKa = 11.84VEE34 pKa = 6.11RR35 pKa = 11.84EE36 pKa = 3.77LQLDD40 pKa = 3.91PEE42 pKa = 4.8HH43 pKa = 7.54GEE45 pKa = 4.04EE46 pKa = 4.18YY47 pKa = 10.33AIHH50 pKa = 7.15DD51 pKa = 3.88FEE53 pKa = 4.49NFYY56 pKa = 11.06GFSVGEE62 pKa = 3.89YY63 pKa = 10.61SSISEE68 pKa = 4.18LNDD71 pKa = 3.12YY72 pKa = 10.8AEE74 pKa = 4.34KK75 pKa = 10.93LEE77 pKa = 4.76EE78 pKa = 5.05ISDD81 pKa = 3.9LDD83 pKa = 4.01HH84 pKa = 6.88IKK86 pKa = 10.7EE87 pKa = 3.9FLEE90 pKa = 4.65IYY92 pKa = 10.49SIDD95 pKa = 5.15DD96 pKa = 4.11IISCKK101 pKa = 10.33DD102 pKa = 3.19DD103 pKa = 3.94LEE105 pKa = 4.37FVEE108 pKa = 6.17AEE110 pKa = 4.21DD111 pKa = 5.91DD112 pKa = 3.57EE113 pKa = 5.95DD114 pKa = 4.39LAAEE118 pKa = 5.02LVAQMGGVEE127 pKa = 4.4TLSQATLEE135 pKa = 4.23QYY137 pKa = 10.91FNYY140 pKa = 10.21AAYY143 pKa = 10.1GRR145 pKa = 11.84DD146 pKa = 3.62FAISDD151 pKa = 3.85YY152 pKa = 10.91SQTSHH157 pKa = 6.88GYY159 pKa = 8.75VRR161 pKa = 11.84NII163 pKa = 3.52

Molecular weight: 18.78 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|F9VGT1|F9VGT1_LACGL Cation-transporting ATPase OS=Lactococcus garvieae (strain Lg2) OX=420890 GN=LCGL_0104 PE=4 SV=1
MM1 pKa = 7.28KK2 pKa = 9.42RR3 pKa = 11.84TYY5 pKa = 10.04QPHH8 pKa = 5.97KK9 pKa = 10.04KK10 pKa = 9.24PRR12 pKa = 11.84KK13 pKa = 4.63TTHH16 pKa = 5.81GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 9.97NGRR28 pKa = 11.84RR29 pKa = 11.84VLAARR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84KK37 pKa = 9.65GRR39 pKa = 11.84ASLTVV44 pKa = 3.04

Molecular weight: 5.18 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1938

0

1938

563232

38

1627

290.6

32.53

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.924 ± 0.068

0.473 ± 0.014

5.293 ± 0.045

7.208 ± 0.067

4.767 ± 0.045

6.659 ± 0.058

1.85 ± 0.023

7.383 ± 0.053

7.007 ± 0.06

9.923 ± 0.073

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.706 ± 0.024

4.692 ± 0.04

3.371 ± 0.03

3.867 ± 0.035

3.863 ± 0.039

6.03 ± 0.044

5.681 ± 0.047

6.851 ± 0.047

0.944 ± 0.026

3.508 ± 0.038
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski