Hubei noda-like virus 8

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 7.91

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A1L3KGR5|A0A1L3KGR5_9VIRU RNA replicase OS=Hubei noda-like virus 8 OX=1922988 PE=3 SV=1
MM1 pKa = 7.31SRR3 pKa = 11.84ISHH6 pKa = 7.1FLTGVTYY13 pKa = 9.19PYY15 pKa = 10.99GCGRR19 pKa = 11.84PSPFDD24 pKa = 3.44VEE26 pKa = 4.11PRR28 pKa = 11.84SWNMPYY34 pKa = 10.61RR35 pKa = 11.84LAKK38 pKa = 9.57ACCYY42 pKa = 9.27LAKK45 pKa = 10.7HH46 pKa = 5.21NVEE49 pKa = 4.54LLSGQNIRR57 pKa = 11.84DD58 pKa = 3.51ILSKK62 pKa = 10.76FKK64 pKa = 10.41TFYY67 pKa = 10.99EE68 pKa = 4.67CVTKK72 pKa = 10.56LWADD76 pKa = 4.93GITLEE81 pKa = 4.51CAIEE85 pKa = 4.28TVVSSTNGLYY95 pKa = 10.17LRR97 pKa = 11.84KK98 pKa = 8.57PTLKK102 pKa = 10.24EE103 pKa = 2.95IRR105 pKa = 11.84YY106 pKa = 8.74VVKK109 pKa = 10.67LYY111 pKa = 11.21LEE113 pKa = 4.24ALFLIITVPPLTQVQIASLLFDD135 pKa = 5.4SIASYY140 pKa = 11.26YY141 pKa = 9.91GIKK144 pKa = 10.49APANRR149 pKa = 11.84HH150 pKa = 4.45SFFVEE155 pKa = 4.32VTSDD159 pKa = 3.69EE160 pKa = 4.41PVLDD164 pKa = 3.62TRR166 pKa = 11.84RR167 pKa = 11.84EE168 pKa = 3.89LMLDD172 pKa = 3.31VVPIRR177 pKa = 11.84RR178 pKa = 11.84TMSNHH183 pKa = 5.0SHH185 pKa = 7.0PSSAAEE191 pKa = 3.68RR192 pKa = 11.84SSVHH196 pKa = 5.56AAIVNKK202 pKa = 9.86IVEE205 pKa = 4.29AGYY208 pKa = 10.76EE209 pKa = 3.95PFDD212 pKa = 3.51ISMSKK217 pKa = 10.28SAIEE221 pKa = 3.94QGLSGQRR228 pKa = 11.84YY229 pKa = 7.78FYY231 pKa = 9.8WLKK234 pKa = 10.6DD235 pKa = 3.68YY236 pKa = 10.59KK237 pKa = 10.7CSVVEE242 pKa = 3.73HH243 pKa = 7.14PIRR246 pKa = 11.84HH247 pKa = 5.63SSVIVMVDD255 pKa = 2.23VDD257 pKa = 4.39YY258 pKa = 11.64YY259 pKa = 11.74LDD261 pKa = 3.28INEE264 pKa = 3.92YY265 pKa = 8.74MKK267 pKa = 10.61YY268 pKa = 10.03FRR270 pKa = 11.84PIVIYY275 pKa = 8.93TLQPLTLRR283 pKa = 11.84FVGPEE288 pKa = 3.37YY289 pKa = 10.82SFRR292 pKa = 11.84VDD294 pKa = 2.92KK295 pKa = 11.67GEE297 pKa = 3.58VFYY300 pKa = 10.45EE301 pKa = 3.91VRR303 pKa = 11.84GGAKK307 pKa = 9.77YY308 pKa = 9.59SHH310 pKa = 6.98KK311 pKa = 9.08IWNYY315 pKa = 7.08STHH318 pKa = 6.59GDD320 pKa = 3.7TVSVRR325 pKa = 11.84DD326 pKa = 3.77WYY328 pKa = 11.31GNQCVFSVEE337 pKa = 3.97KK338 pKa = 10.68QLVGKK343 pKa = 9.13PDD345 pKa = 3.64EE346 pKa = 4.72GSSRR350 pKa = 11.84HH351 pKa = 6.05IIYY354 pKa = 10.29LNPIRR359 pKa = 11.84RR360 pKa = 11.84IPWFCITEE368 pKa = 3.73KK369 pKa = 10.98DD370 pKa = 3.75MPLVRR375 pKa = 11.84HH376 pKa = 7.06DD377 pKa = 3.84YY378 pKa = 9.54TLSTRR383 pKa = 11.84RR384 pKa = 11.84PLVEE388 pKa = 4.26TPSITTTTTEE398 pKa = 4.18KK399 pKa = 10.94KK400 pKa = 10.04PDD402 pKa = 3.67GTVKK406 pKa = 10.32TITNKK411 pKa = 9.06TKK413 pKa = 10.37QKK415 pKa = 10.22EE416 pKa = 4.41SVIEE420 pKa = 3.94KK421 pKa = 10.08LGNSLWDD428 pKa = 3.39SFNYY432 pKa = 9.7GVSEE436 pKa = 4.03GFNYY440 pKa = 10.15ILDD443 pKa = 5.08PIDD446 pKa = 4.04NYY448 pKa = 10.73IEE450 pKa = 4.1LAEE453 pKa = 4.57SNSFTSMRR461 pKa = 11.84LTIPEE466 pKa = 4.17FEE468 pKa = 4.43SFLTRR473 pKa = 11.84AEE475 pKa = 4.14IKK477 pKa = 9.68PLKK480 pKa = 10.16PGDD483 pKa = 4.02IEE485 pKa = 4.4LQLKK489 pKa = 9.54KK490 pKa = 10.39RR491 pKa = 11.84YY492 pKa = 8.79SEE494 pKa = 4.31KK495 pKa = 10.5KK496 pKa = 9.66GEE498 pKa = 3.97AMPYY502 pKa = 9.73EE503 pKa = 4.95EE504 pKa = 4.91IVKK507 pKa = 9.06TPIIAKK513 pKa = 9.67ILSNVGKK520 pKa = 7.45TVKK523 pKa = 10.79GNTTPTNYY531 pKa = 9.86IQMKK535 pKa = 9.9QSTTVEE541 pKa = 4.16TANLDD546 pKa = 3.73SQVFTVEE553 pKa = 3.81GKK555 pKa = 10.4DD556 pKa = 3.01IFFEE560 pKa = 4.57SKK562 pKa = 10.88LPGQVITSCLVRR574 pKa = 11.84IPAVLPSRR582 pKa = 11.84TRR584 pKa = 11.84NNNQASIMGRR594 pKa = 11.84ISEE597 pKa = 4.21LHH599 pKa = 6.26NDD601 pKa = 3.63VVPTVLNHH609 pKa = 6.63LRR611 pKa = 11.84FNSAATEE618 pKa = 4.41FVRR621 pKa = 11.84LMVPEE626 pKa = 4.74IGHH629 pKa = 5.36GVEE632 pKa = 4.02YY633 pKa = 10.9SIDD636 pKa = 3.62QVVAKK641 pKa = 9.9QDD643 pKa = 2.98KK644 pKa = 8.75CAQRR648 pKa = 11.84QRR650 pKa = 11.84INEE653 pKa = 4.25IINTFSAEE661 pKa = 3.82PTNRR665 pKa = 11.84LTTFIKK671 pKa = 10.44NEE673 pKa = 3.86AYY675 pKa = 10.42ASVNDD680 pKa = 3.84PRR682 pKa = 11.84NITTMSSNFTLIASKK697 pKa = 8.84ITYY700 pKa = 9.34AFKK703 pKa = 10.8DD704 pKa = 3.52AVLKK708 pKa = 7.44QHH710 pKa = 5.91KK711 pKa = 9.21WYY713 pKa = 10.73GPGKK717 pKa = 7.98TPSEE721 pKa = 4.29SVDD724 pKa = 3.51LLRR727 pKa = 11.84NICANGACLTDD738 pKa = 3.12YY739 pKa = 10.96SRR741 pKa = 11.84YY742 pKa = 9.87DD743 pKa = 3.27GRR745 pKa = 11.84VSKK748 pKa = 10.12WLQNRR753 pKa = 11.84IAKK756 pKa = 9.53AAYY759 pKa = 9.49LRR761 pKa = 11.84WASPGDD767 pKa = 3.33TKK769 pKa = 11.5EE770 pKa = 4.34NVNHH774 pKa = 5.61MFKK777 pKa = 10.61EE778 pKa = 4.13IFRR781 pKa = 11.84LTGRR785 pKa = 11.84TADD788 pKa = 3.5GEE790 pKa = 4.41LYY792 pKa = 10.54YY793 pKa = 10.64AGHH796 pKa = 6.07GTRR799 pKa = 11.84SGSPITTDD807 pKa = 3.04ANTMINAYY815 pKa = 9.47IAFCALLYY823 pKa = 10.57LGYY826 pKa = 10.43DD827 pKa = 3.51SKK829 pKa = 10.31TSFALLGLYY838 pKa = 10.43AGDD841 pKa = 5.04DD842 pKa = 3.71GVTPYY847 pKa = 10.56IPGIEE852 pKa = 3.87TALYY856 pKa = 9.58KK857 pKa = 10.75VCTALGMVVKK867 pKa = 10.69LEE869 pKa = 3.85VSPIGEE875 pKa = 4.41EE876 pKa = 3.56INYY879 pKa = 9.88LGRR882 pKa = 11.84IFPNILVSDD891 pKa = 4.16ISYY894 pKa = 10.98QDD896 pKa = 3.39IKK898 pKa = 10.63RR899 pKa = 11.84TLPKK903 pKa = 10.54LHH905 pKa = 7.07ISTNQQVKK913 pKa = 10.31RR914 pKa = 11.84EE915 pKa = 3.95VAAVNKK921 pKa = 10.23ALGYY925 pKa = 8.39LTTDD929 pKa = 4.66AITPIIGSWAKK940 pKa = 10.05RR941 pKa = 11.84VLDD944 pKa = 4.41LNPDD948 pKa = 3.57VACRR952 pKa = 11.84NFLSEE957 pKa = 4.35EE958 pKa = 3.94LFKK961 pKa = 10.77IEE963 pKa = 4.26NGAWPQPTQPEE974 pKa = 4.2DD975 pKa = 3.4LDD977 pKa = 5.37LIVEE981 pKa = 4.67SICKK985 pKa = 9.5HH986 pKa = 6.73LDD988 pKa = 3.23MTPSEE993 pKa = 4.56LEE995 pKa = 4.32CFDD998 pKa = 3.59EE999 pKa = 6.44AIRR1002 pKa = 11.84TSDD1005 pKa = 3.56TLCTHH1010 pKa = 7.33LDD1012 pKa = 3.42YY1013 pKa = 11.32FDD1015 pKa = 4.57IVWEE1019 pKa = 4.15NEE1021 pKa = 4.08VEE1023 pKa = 4.48HH1024 pKa = 6.81KK1025 pKa = 10.66RR1026 pKa = 11.84PAYY1029 pKa = 10.54LNGEE1033 pKa = 4.25IVGTGQPSYY1042 pKa = 10.56TVGLTPATNINQCQINQLITPPPPSKK1068 pKa = 10.1IKK1070 pKa = 10.46KK1071 pKa = 8.98EE1072 pKa = 3.98KK1073 pKa = 9.7EE1074 pKa = 3.87KK1075 pKa = 11.06KK1076 pKa = 10.18KK1077 pKa = 10.46NASNKK1082 pKa = 9.62KK1083 pKa = 8.25KK1084 pKa = 9.38QQSSGSISPEE1094 pKa = 3.57PVAVPSTSEE1103 pKa = 4.6DD1104 pKa = 3.61FPQIATHH1111 pKa = 5.87SRR1113 pKa = 11.84SGTGSISSSSDD1124 pKa = 2.85KK1125 pKa = 10.04QTTSSSPKK1133 pKa = 9.63RR1134 pKa = 11.84EE1135 pKa = 3.68RR1136 pKa = 11.84KK1137 pKa = 9.55LSPKK1141 pKa = 8.06STKK1144 pKa = 9.76KK1145 pKa = 10.37RR1146 pKa = 11.84SEE1148 pKa = 3.74KK1149 pKa = 10.66SKK1151 pKa = 10.98ASRR1154 pKa = 11.84TLSTPSTT1161 pKa = 3.77

Molecular weight:
130.67 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A1L3KGR5|A0A1L3KGR5_9VIRU RNA replicase OS=Hubei noda-like virus 8 OX=1922988 PE=3 SV=1
MM1 pKa = 7.67PQQQNNTPALNVNLNTNGRR20 pKa = 11.84PSRR23 pKa = 11.84SRR25 pKa = 11.84SRR27 pKa = 11.84KK28 pKa = 8.58KK29 pKa = 9.92RR30 pKa = 11.84SKK32 pKa = 10.16RR33 pKa = 11.84SQSRR37 pKa = 11.84SASNVIRR44 pKa = 11.84NRR46 pKa = 11.84VMLGKK51 pKa = 10.12NYY53 pKa = 9.72TGNPLPGDD61 pKa = 3.77VLFALPPNATRR72 pKa = 11.84SGFKK76 pKa = 10.19PNPADD81 pKa = 3.68PRR83 pKa = 11.84TFDD86 pKa = 4.07AFTNLGNTSTGRR98 pKa = 11.84AAAQKK103 pKa = 9.8ILHH106 pKa = 6.28PCEE109 pKa = 4.68EE110 pKa = 4.41GAQPFVRR117 pKa = 11.84FPDD120 pKa = 3.95GAASITTAFEE130 pKa = 3.84RR131 pKa = 11.84RR132 pKa = 11.84DD133 pKa = 3.25EE134 pKa = 4.24YY135 pKa = 10.8MISRR139 pKa = 11.84QYY141 pKa = 9.89TADD144 pKa = 3.22SNMNILVVHH153 pKa = 6.37LPFLRR158 pKa = 11.84YY159 pKa = 9.23NRR161 pKa = 11.84LVIQWDD167 pKa = 4.07SSQSPTDD174 pKa = 3.53YY175 pKa = 11.31DD176 pKa = 3.71LQTVFTEE183 pKa = 4.05ALTGVAIYY191 pKa = 9.0PSWNTKK197 pKa = 5.9TTGDD201 pKa = 3.38TSDD204 pKa = 3.32QITYY208 pKa = 8.01YY209 pKa = 8.07TTILSPNVLTPGGGGTPEE227 pKa = 3.87AARR230 pKa = 11.84SYY232 pKa = 10.1LRR234 pKa = 11.84NVRR237 pKa = 11.84RR238 pKa = 11.84VYY240 pKa = 10.75YY241 pKa = 10.73GSTQDD246 pKa = 4.52LDD248 pKa = 3.79ASSLYY253 pKa = 10.85DD254 pKa = 3.29EE255 pKa = 4.92GRR257 pKa = 11.84VVAGQWSPDD266 pKa = 3.17VVLGINRR273 pKa = 11.84ARR275 pKa = 11.84DD276 pKa = 3.46NTVQNSCYY284 pKa = 9.64RR285 pKa = 11.84LSVPALTSEE294 pKa = 5.46SIVQSDD300 pKa = 4.04AYY302 pKa = 10.39RR303 pKa = 11.84YY304 pKa = 8.68QALAKK309 pKa = 8.37TGCYY313 pKa = 8.95MPLRR317 pKa = 11.84PSDD320 pKa = 3.97PDD322 pKa = 3.54VQFSGASEE330 pKa = 3.86QRR332 pKa = 11.84PLEE335 pKa = 4.15IAFHH339 pKa = 5.53QSGNITGNLTTVDD352 pKa = 4.75DD353 pKa = 3.25IWLRR357 pKa = 11.84GWNIGVEE364 pKa = 4.06LWTNIDD370 pKa = 3.0YY371 pKa = 10.29RR372 pKa = 11.84ANLRR376 pKa = 11.84YY377 pKa = 7.98KK378 pKa = 8.85TCEE381 pKa = 3.83GLEE384 pKa = 4.21LSPSANSLYY393 pKa = 10.07STFEE397 pKa = 3.57GRR399 pKa = 11.84GYY401 pKa = 10.13PRR403 pKa = 11.84DD404 pKa = 3.82DD405 pKa = 3.5RR406 pKa = 11.84ALSVIRR412 pKa = 11.84EE413 pKa = 4.17FARR416 pKa = 11.84SQPHH420 pKa = 7.47AYY422 pKa = 9.15PADD425 pKa = 4.08FNDD428 pKa = 5.34KK429 pKa = 10.69NLLWGQLIRR438 pKa = 11.84GLGTALSSLGLPILSQVAEE457 pKa = 4.48PVAHH461 pKa = 7.4AIANYY466 pKa = 8.51VEE468 pKa = 4.21RR469 pKa = 5.89

Molecular weight:
52.07 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

1630

469

1161

815.0

91.37

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.258 ± 0.893

1.411 ± 0.27

4.847 ± 0.337

5.583 ± 0.948

3.313 ± 0.159

5.583 ± 0.6

1.718 ± 0.315

6.503 ± 1.083

5.583 ± 1.67

8.037 ± 0.341

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.411 ± 0.167

5.337 ± 0.719

6.012 ± 0.289

3.436 ± 0.607

6.012 ± 1.115

8.896 ± 0.029

7.73 ± 0.129

6.503 ± 0.361

1.227 ± 0.129

4.601 ± 0.043

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski