Bacillus phage BSP9

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Herelleviridae; Bastillevirinae; Nitunavirus; unclassified Nitunavirus

Average proteome isoelectric point is 5.97

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 228 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A384X9I4|A0A384X9I4_9CAUD Uncharacterized protein OS=Bacillus phage BSP9 OX=2041339 GN=BSP9_164 PE=4 SV=1
MM1 pKa = 7.65TNTNIQWAVNIPEE14 pKa = 5.8DD15 pKa = 4.1NIQWAVNILEE25 pKa = 4.71DD26 pKa = 4.19NMEE29 pKa = 4.24GMSKK33 pKa = 10.19EE34 pKa = 3.84EE35 pKa = 4.2KK36 pKa = 10.03INYY39 pKa = 9.59LEE41 pKa = 5.16DD42 pKa = 3.33VTSYY46 pKa = 11.28GCVSGCVSGAIYY58 pKa = 10.62YY59 pKa = 10.31FEE61 pKa = 6.49LDD63 pKa = 4.4AIFTANLHH71 pKa = 6.96DD72 pKa = 3.57ILEE75 pKa = 4.42RR76 pKa = 11.84LDD78 pKa = 4.17EE79 pKa = 4.45IKK81 pKa = 10.66EE82 pKa = 3.92EE83 pKa = 3.86HH84 pKa = 7.38DD85 pKa = 3.48YY86 pKa = 11.65DD87 pKa = 3.93VLHH90 pKa = 7.27EE91 pKa = 4.04YY92 pKa = 9.57MEE94 pKa = 4.5RR95 pKa = 11.84QNKK98 pKa = 9.81GYY100 pKa = 9.28TDD102 pKa = 3.94FAEE105 pKa = 4.27SAVWLIVEE113 pKa = 4.36NTASRR118 pKa = 11.84LLADD122 pKa = 4.56LEE124 pKa = 4.28NEE126 pKa = 4.06EE127 pKa = 4.53EE128 pKa = 4.32EE129 pKa = 4.41EE130 pKa = 4.28EE131 pKa = 4.31EE132 pKa = 4.26

Molecular weight: 15.36 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A384X9C2|A0A384X9C2_9CAUD Putative ribonucleotide reductase stimulatory protein OS=Bacillus phage BSP9 OX=2041339 GN=BSP9_016 PE=3 SV=1
MM1 pKa = 7.02SHH3 pKa = 6.85PVAVIIACAVGIVLVRR19 pKa = 11.84VTMDD23 pKa = 2.7IRR25 pKa = 11.84EE26 pKa = 4.2KK27 pKa = 10.95ASNKK31 pKa = 8.92HH32 pKa = 4.3NRR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84DD37 pKa = 3.22

Molecular weight: 4.16 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

228

0

228

44425

37

1391

194.8

22.13

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.093 ± 0.224

0.763 ± 0.065

6.584 ± 0.155

8.189 ± 0.295

3.908 ± 0.102

6.328 ± 0.242

1.895 ± 0.115

6.026 ± 0.13

7.633 ± 0.212

8.306 ± 0.176

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.584 ± 0.087

5.171 ± 0.152

3.212 ± 0.14

3.66 ± 0.145

4.612 ± 0.119

6.357 ± 0.222

6.251 ± 0.213

6.926 ± 0.145

1.042 ± 0.062

4.459 ± 0.13
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski