Kangiella sp. HZ709

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Oceanospirillales; Kangiellaceae; Kangiella; unclassified Kangiella

Average proteome isoelectric point is 6.28

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2214 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6L5QVQ9|A0A6L5QVQ9_9GAMM Enolase OS=Kangiella sp. HZ709 OX=2666328 GN=eno PE=3 SV=1
MM1 pKa = 7.22SLKK4 pKa = 10.45KK5 pKa = 10.78VFLATTLALSSGITLASGVSYY26 pKa = 10.84DD27 pKa = 3.88YY28 pKa = 11.61LDD30 pKa = 3.93VSLQEE35 pKa = 3.9YY36 pKa = 10.68DD37 pKa = 3.67GADD40 pKa = 3.39GYY42 pKa = 11.68NFEE45 pKa = 4.73LSKK48 pKa = 11.11SFSSNLYY55 pKa = 10.34GRR57 pKa = 11.84IDD59 pKa = 3.69YY60 pKa = 9.89TDD62 pKa = 4.22LDD64 pKa = 4.04GDD66 pKa = 3.93FGGEE70 pKa = 4.14FSATRR75 pKa = 11.84LNLGYY80 pKa = 10.13LASLNSSVDD89 pKa = 4.28FIAEE93 pKa = 4.38LGYY96 pKa = 11.06EE97 pKa = 4.49DD98 pKa = 5.11IDD100 pKa = 4.8VGVADD105 pKa = 5.24DD106 pKa = 3.97NGFNARR112 pKa = 11.84LGFRR116 pKa = 11.84GMATNQLEE124 pKa = 4.35LGAFASYY131 pKa = 11.32SDD133 pKa = 3.92VLEE136 pKa = 4.42STDD139 pKa = 2.97ITVEE143 pKa = 3.94GRR145 pKa = 11.84YY146 pKa = 9.72HH147 pKa = 6.02FTDD150 pKa = 3.4QLSLALEE157 pKa = 4.26IGNDD161 pKa = 3.73DD162 pKa = 4.72EE163 pKa = 6.44LDD165 pKa = 3.25EE166 pKa = 5.22HH167 pKa = 7.95YY168 pKa = 10.5GVSVRR173 pKa = 11.84YY174 pKa = 10.14SFF176 pKa = 4.88

Molecular weight: 19.23 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6L5QVM1|A0A6L5QVM1_9GAMM Imidazoleglycerol-phosphate dehydratase OS=Kangiella sp. HZ709 OX=2666328 GN=hisB PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNIKK11 pKa = 10.16RR12 pKa = 11.84KK13 pKa = 7.88RR14 pKa = 11.84THH16 pKa = 5.91GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.3NGRR28 pKa = 11.84AILNRR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.75GRR39 pKa = 11.84KK40 pKa = 8.85RR41 pKa = 11.84LSAA44 pKa = 3.96

Molecular weight: 5.22 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2214

0

2214

734385

37

6386

331.7

37.01

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.119 ± 0.053

0.821 ± 0.017

5.674 ± 0.045

6.532 ± 0.057

4.344 ± 0.044

6.667 ± 0.057

2.002 ± 0.024

6.943 ± 0.054

6.462 ± 0.051

10.076 ± 0.074

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.408 ± 0.032

4.858 ± 0.05

3.694 ± 0.034

4.398 ± 0.042

4.113 ± 0.037

6.825 ± 0.051

5.138 ± 0.056

6.443 ± 0.043

1.207 ± 0.019

3.277 ± 0.049
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski