Leviviridae sp.

Taxonomy: Viruses; Riboviria; Orthornavirae; Lenarviricota; Allassoviricetes; Levivirales; Leviviridae; unclassified Leviviridae

Average proteome isoelectric point is 6.64

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A142D861|A0A142D861_9VIRU RNA replicase beta chain OS=Leviviridae sp. OX=2027243 PE=4 SV=1
MM1 pKa = 7.58SKK3 pKa = 9.59TSVIDD8 pKa = 3.46ATNTTASTTFNASAYY23 pKa = 8.94KK24 pKa = 10.57LSVLGNYY31 pKa = 10.18ALTADD36 pKa = 4.45EE37 pKa = 4.54PTEE40 pKa = 3.88ATYY43 pKa = 11.63SNVTSSIEE51 pKa = 4.0RR52 pKa = 11.84PEE54 pKa = 3.85LLQYY58 pKa = 10.54RR59 pKa = 11.84SRR61 pKa = 11.84RR62 pKa = 11.84SEE64 pKa = 3.93KK65 pKa = 10.88NNTGLNVPNVSDD77 pKa = 3.25TAGYY81 pKa = 9.34VEE83 pKa = 4.52YY84 pKa = 10.54GIRR87 pKa = 11.84LQNVVHH93 pKa = 5.72TTDD96 pKa = 3.06TADD99 pKa = 3.62ANVEE103 pKa = 4.03IADD106 pKa = 4.05PCCVNITLKK115 pKa = 10.89HH116 pKa = 5.86PATSRR121 pKa = 11.84ITEE124 pKa = 4.76DD125 pKa = 2.86IATQLLMRR133 pKa = 11.84AVSTMFDD140 pKa = 3.41DD141 pKa = 4.33AGKK144 pKa = 10.63SRR146 pKa = 11.84LGDD149 pKa = 3.32MMRR152 pKa = 11.84SALHH156 pKa = 5.18ITANN160 pKa = 3.56

Molecular weight: 17.37 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A142D861|A0A142D861_9VIRU RNA replicase beta chain OS=Leviviridae sp. OX=2027243 PE=4 SV=1
MM1 pKa = 7.22YY2 pKa = 10.62LNRR5 pKa = 11.84DD6 pKa = 3.57TVKK9 pKa = 10.78KK10 pKa = 10.33ILALDD15 pKa = 3.74NVEE18 pKa = 4.08VTEE21 pKa = 5.29DD22 pKa = 3.7NIFSAKK28 pKa = 9.87LALEE32 pKa = 4.52VWQSLLSEE40 pKa = 4.3QNPQYY45 pKa = 11.15GKK47 pKa = 10.82LVLSYY52 pKa = 11.06ALRR55 pKa = 11.84YY56 pKa = 8.98GLRR59 pKa = 11.84RR60 pKa = 11.84TIQEE64 pKa = 3.91ACDD67 pKa = 3.53CSEE70 pKa = 3.8ILMGRR75 pKa = 11.84SFRR78 pKa = 11.84NNAFTGIVATATDD91 pKa = 3.94EE92 pKa = 4.8RR93 pKa = 11.84SVLQCLRR100 pKa = 11.84FLKK103 pKa = 10.31RR104 pKa = 11.84FCYY107 pKa = 10.16PDD109 pKa = 4.95DD110 pKa = 5.25DD111 pKa = 4.39SLRR114 pKa = 11.84EE115 pKa = 3.88EE116 pKa = 4.42AVSQFFKK123 pKa = 10.14RR124 pKa = 11.84THH126 pKa = 5.0GWAQLKK132 pKa = 10.44ISDD135 pKa = 4.16YY136 pKa = 10.02LRR138 pKa = 11.84SVIKK142 pKa = 10.71SKK144 pKa = 10.83FSDD147 pKa = 3.01IVGSEE152 pKa = 3.57KK153 pKa = 10.41NYY155 pKa = 10.15RR156 pKa = 11.84RR157 pKa = 11.84SEE159 pKa = 4.08VANLTKK165 pKa = 10.35SYY167 pKa = 11.17FDD169 pKa = 3.79IPSGAAYY176 pKa = 10.16DD177 pKa = 3.65IEE179 pKa = 4.35YY180 pKa = 10.83DD181 pKa = 3.59RR182 pKa = 11.84LVRR185 pKa = 11.84TQGEE189 pKa = 4.64KK190 pKa = 9.02ITILNEE196 pKa = 3.67YY197 pKa = 9.01VKK199 pKa = 10.0YY200 pKa = 10.19HH201 pKa = 6.55PSYY204 pKa = 10.37PGIKK208 pKa = 9.52RR209 pKa = 11.84FVYY212 pKa = 10.23DD213 pKa = 3.16QSKK216 pKa = 10.95DD217 pKa = 3.34FTPISKK223 pKa = 10.68LIAVPKK229 pKa = 9.69NYY231 pKa = 7.92KK232 pKa = 7.08TSRR235 pKa = 11.84VIAVEE240 pKa = 4.01HH241 pKa = 6.63PLVQFRR247 pKa = 11.84LGAISNYY254 pKa = 10.18LSDD257 pKa = 6.07RR258 pKa = 11.84IMKK261 pKa = 7.21YY262 pKa = 8.5TKK264 pKa = 10.47GRR266 pKa = 11.84INIQDD271 pKa = 3.13QSRR274 pKa = 11.84NRR276 pKa = 11.84NLAQLGSAQDD286 pKa = 3.48SYY288 pKa = 11.95ATIDD292 pKa = 3.81LSAASDD298 pKa = 3.98SNTQMLQYY306 pKa = 9.59QICPDD311 pKa = 2.91WLYY314 pKa = 11.33RR315 pKa = 11.84EE316 pKa = 3.97ISPWISRR323 pKa = 11.84RR324 pKa = 11.84VRR326 pKa = 11.84YY327 pKa = 9.4NGKK330 pKa = 6.2TRR332 pKa = 11.84RR333 pKa = 11.84VNTFGTMGTRR343 pKa = 11.84ITFPLEE349 pKa = 3.69MVTFIAVVEE358 pKa = 4.31VARR361 pKa = 11.84DD362 pKa = 3.72YY363 pKa = 11.74YY364 pKa = 9.68NTLVGEE370 pKa = 3.96PHH372 pKa = 6.32ITLDD376 pKa = 4.6DD377 pKa = 3.58IAVYY381 pKa = 10.59GDD383 pKa = 4.61DD384 pKa = 3.76IIVPKK389 pKa = 10.29EE390 pKa = 3.84LVSTVSDD397 pKa = 3.63LLGILGFTVNAEE409 pKa = 4.15KK410 pKa = 9.89TYY412 pKa = 10.51SSGPFRR418 pKa = 11.84EE419 pKa = 4.46SCGGDD424 pKa = 3.45YY425 pKa = 11.29YY426 pKa = 11.64NGVDD430 pKa = 3.57VSTNYY435 pKa = 9.4WPRR438 pKa = 11.84TPIRR442 pKa = 11.84RR443 pKa = 11.84DD444 pKa = 2.74ISTIPSLISLQHH456 pKa = 6.4RR457 pKa = 11.84LVQHH461 pKa = 6.23FEE463 pKa = 4.39SNLILVNTIRR473 pKa = 11.84DD474 pKa = 3.7IVPSITEE481 pKa = 4.02SEE483 pKa = 3.97IGSPYY488 pKa = 8.91TDD490 pKa = 3.39IWSAWPVLTLRR501 pKa = 11.84EE502 pKa = 4.12GRR504 pKa = 11.84EE505 pKa = 3.39RR506 pKa = 11.84RR507 pKa = 11.84YY508 pKa = 10.87ALTYY512 pKa = 10.99SKK514 pKa = 10.49FSDD517 pKa = 3.51AMRR520 pKa = 11.84PDD522 pKa = 3.24GKK524 pKa = 10.52RR525 pKa = 11.84YY526 pKa = 8.74VAVRR530 pKa = 11.84RR531 pKa = 11.84TKK533 pKa = 10.31KK534 pKa = 9.01IHH536 pKa = 5.97HH537 pKa = 6.21VQTATAEE544 pKa = 4.19YY545 pKa = 10.22HH546 pKa = 5.6STFPPKK552 pKa = 9.48WKK554 pKa = 10.47NGWPSADD561 pKa = 2.94IQEE564 pKa = 4.25FLMYY568 pKa = 10.29RR569 pKa = 11.84SLYY572 pKa = 10.62HH573 pKa = 6.1GGCYY577 pKa = 10.2ASGLDD582 pKa = 3.85EE583 pKa = 4.93LLHH586 pKa = 5.53VTDD589 pKa = 5.69KK590 pKa = 10.82PLHH593 pKa = 5.48QEE595 pKa = 3.24WDD597 pKa = 3.76LPILDD602 pKa = 4.25RR603 pKa = 11.84DD604 pKa = 3.8HH605 pKa = 7.48VRR607 pKa = 11.84VLIEE611 pKa = 3.63

Molecular weight: 70.37 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

1230

160

611

410.0

46.71

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.26 ± 1.052

1.545 ± 0.324

6.098 ± 0.333

5.854 ± 0.58

3.74 ± 0.814

5.528 ± 0.796

1.951 ± 0.113

5.772 ± 1.012

5.285 ± 0.76

8.537 ± 0.258

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.545 ± 0.385

4.228 ± 0.824

3.415 ± 0.546

3.252 ± 0.336

7.236 ± 0.44

8.374 ± 0.269

7.642 ± 1.326

6.911 ± 0.031

1.382 ± 0.333

5.447 ± 0.416
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski