Botryosphaeria parva (strain UCR-NP2) (Grapevine canker fungus) (Neofusicoccum parvum)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Pezizomycotina; leotiomyceta; dothideomyceta; Dothideomycetes; Dothideomycetes incertae sedis; Botryosphaeriales; Botryosphaeriaceae; Neofusicoccum; Neofusicoccum parvum

Average proteome isoelectric point is 6.25

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 10365 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R1ERX5|R1ERX5_BOTPV Putative outer membrane autotransporter protein OS=Botryosphaeria parva (strain UCR-NP2) OX=1287680 GN=UCRNP2_2714 PE=4 SV=1
MM1 pKa = 7.46FFNILFDD8 pKa = 3.84LFFNVFFDD16 pKa = 3.6IFLDD20 pKa = 3.35MFFNIFFEE28 pKa = 4.12IFFDD32 pKa = 3.14ILLNIFFNFFPNILLSIFLNVFDD55 pKa = 4.46IFFNIFSYY63 pKa = 10.74IVFDD67 pKa = 4.03TVFGASTTISDD78 pKa = 3.95LLIPVFAFFTVIYY91 pKa = 9.97FFDD94 pKa = 3.57ILPRR98 pKa = 11.84LLLHH102 pKa = 7.08AFNIPDD108 pKa = 4.01TLLSTLDD115 pKa = 3.69FFDD118 pKa = 3.37ILHH121 pKa = 6.68RR122 pKa = 11.84LFSIFQLSQATIFLNLYY139 pKa = 9.88FSRR142 pKa = 11.84LCFII146 pKa = 5.38

Molecular weight: 17.6 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R1EK03|R1EK03_BOTPV Putative transcription initiation factor 31kd protein OS=Botryosphaeria parva (strain UCR-NP2) OX=1287680 GN=UCRNP2_5394 PE=3 SV=1
MM1 pKa = 7.6RR2 pKa = 11.84PSPRR6 pKa = 11.84LWAFNAYY13 pKa = 9.88CSVHH17 pKa = 4.85TVLYY21 pKa = 10.41CRR23 pKa = 11.84ARR25 pKa = 11.84NSAPAFGVGLVSAWSLLWTFTLLVVHH51 pKa = 7.34DD52 pKa = 4.73AQTDD56 pKa = 4.04FARR59 pKa = 11.84IEE61 pKa = 4.16RR62 pKa = 11.84TEE64 pKa = 4.04GASASLRR71 pKa = 11.84ISNGSATPNGSAQNGKK87 pKa = 8.52QEE89 pKa = 4.07QEE91 pKa = 3.85SRR93 pKa = 11.84AVSTIKK99 pKa = 10.91DD100 pKa = 3.04VDD102 pKa = 3.82LAAEE106 pKa = 4.23RR107 pKa = 11.84KK108 pKa = 9.91KK109 pKa = 11.04LGTTAGPTQRR119 pKa = 11.84RR120 pKa = 11.84GTFAWQHH127 pKa = 5.44YY128 pKa = 8.12PLSPFIEE135 pKa = 4.33RR136 pKa = 11.84LDD138 pKa = 3.45WVADD142 pKa = 3.42VFCNFRR148 pKa = 11.84GMGWNWRR155 pKa = 11.84ISGIAPPPRR164 pKa = 11.84WVQQQLHH171 pKa = 6.47EE172 pKa = 4.35NSGTPISSTPDD183 pKa = 2.88NHH185 pKa = 6.97VGVDD189 pKa = 3.68GTVQPQTRR197 pKa = 11.84SEE199 pKa = 3.97ALRR202 pKa = 11.84AGWRR206 pKa = 11.84TFITGYY212 pKa = 10.35LALDD216 pKa = 3.88LLKK219 pKa = 10.83VITIHH224 pKa = 7.61DD225 pKa = 4.2PYY227 pKa = 11.15FWGLTTAPGPSFLPPFIRR245 pKa = 11.84HH246 pKa = 5.78SPTLLRR252 pKa = 11.84TFRR255 pKa = 11.84LIVSLFAIKK264 pKa = 8.98TALTTIFAMAPLFFIGLLGPRR285 pKa = 11.84AIGARR290 pKa = 11.84ASPWMYY296 pKa = 10.19PDD298 pKa = 3.29TFGSFRR304 pKa = 11.84SVLDD308 pKa = 3.51HH309 pKa = 7.16GLAGWWGSWWHH320 pKa = 4.2QTFRR324 pKa = 11.84FAFSAPSALLARR336 pKa = 11.84SLGLDD341 pKa = 3.01RR342 pKa = 11.84RR343 pKa = 11.84GAPARR348 pKa = 11.84ALQLLVAFALSGCLHH363 pKa = 6.88AAGSHH368 pKa = 4.5TQAGATRR375 pKa = 11.84PLSSFLFFVLQAAAIVAQQALARR398 pKa = 11.84VPVVRR403 pKa = 11.84RR404 pKa = 11.84VPKK407 pKa = 10.43GVRR410 pKa = 11.84QLANFVVVHH419 pKa = 4.92VWFYY423 pKa = 9.56YY424 pKa = 8.2TAPLLADD431 pKa = 4.41DD432 pKa = 5.11FAHH435 pKa = 6.49GGIWLFEE442 pKa = 4.15PVPLSPLRR450 pKa = 11.84GMLGLGGKK458 pKa = 8.39HH459 pKa = 4.85WWEE462 pKa = 3.85SGIAFF467 pKa = 4.8

Molecular weight: 51.5 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

10365

0

10365

4404707

66

10721

425.0

46.78

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.98 ± 0.03

1.15 ± 0.01

5.903 ± 0.021

6.249 ± 0.028

3.841 ± 0.016

7.324 ± 0.023

2.272 ± 0.01

4.604 ± 0.017

4.852 ± 0.025

8.75 ± 0.022

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.167 ± 0.01

3.542 ± 0.016

5.759 ± 0.022

3.77 ± 0.015

5.822 ± 0.022

7.284 ± 0.024

5.854 ± 0.017

6.474 ± 0.021

1.537 ± 0.009

2.867 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski